First and second electrode liquid seal members are arranged between a first electrode and a second electrode. First and second ion exchange membranes are arranged between a first electrode liquid seal member and a second electrode liquid seal member. An eluent seal member is arranged between a first ion exchange membrane and a second ion exchange membrane. Ion exchange is performed between an eluent that passes through an eluent flow path of the eluent seal member from a separation column and an electrode liquid that passes through each of electrode liquid flow paths of the first and second electrode liquid seal members. In a first surface of the eluent seal member that comes into contact with the first ion exchange membrane, a first projection that surrounds the entire circumference of the eluent flow path to extend along the edge of the eluent flow path and projects toward the first ion exchange membrane is formed.

A chromatography system includes an electrolytic eluent generator; a first valve configured to switch between an operating position which directs an output of the electrolytic eluent generator to a continuously generated trap column and a waste position which directs the output of the electrolytic eluent generator to waste; the continuously regenerated trap column; a degasser; a sample injector including a sample injector valve assembly, the sample injector valve assembly configured to switch between an operation mode which directs an output of the degasser to a separation column, a load mode which loads a sample onto the separation column, and a regenerant mode which directs the output of the degasser to a regenerant line; the separation column; a suppressor; and a detector.

A separation time of an isomer of one or more isomers of a sialylated glycopeptide of a sample is calculated from a peak of a precursor XIC. Product ion intensities of the first group are summed at the separation time producing a first sum and product ion intensities of the second group are summed at the separation time producing a second sum using XICs of the first and second groups. A ratio of the first sum to the second sum is calculated. The ratio at the separation time is compared to predetermined ratio ranges that each corresponds to a combination of a selection from a set of the first linkage and the second linkage taken one or more times. One or more linkages of the sialic acid to the glycan of the isomer are identified from a combination found to match the ratio in the comparison.

A separation time of an isomer of one or more isomers of a sialylated glycopeptide of a sample is calculated from a peak of a precursor XIC. Product ion intensities of the first group are summed at the separation time producing a first sum and product ion intensities of the second group are summed at the separation time producing a second sum using XICs of the first and second groups. A ratio of the first sum to the second sum is calculated. The ratio at the separation time is compared to predetermined ratio ranges that each corresponds to a combination of a selection from a set of the first linkage and the second linkage taken one or more times. One or more linkages of the sialic acid to the glycan of the isomer are identified from a combination found to match the ratio in the comparison.

Disclosed herein are an amino acid analysis method and a liquid chromatographic apparatus for improving separation performance of threonine, serine, glycine, and alanine. The method of analyzing amino acids using the liquid chromatographic apparatus equipped with a cation exchange column includes a process for distributing a sample containing threonine, serine, glycine, and alanine as the amino acids, together with an eluent, to the cation exchange column to separate threonine, serine, glycine, and alanine, wherein a column temperature when separating threonine and serine is higher than a column temperature when separating glycine and alanine.

Disclosed herein are an amino acid analysis method and a liquid chromatographic apparatus for improving separation performance of threonine, serine, glycine, and alanine. The method of analyzing amino acids using the liquid chromatographic apparatus equipped with a cation exchange column includes a process for distributing a sample containing threonine, serine, glycine, and alanine as the amino acids, together with an eluent, to the cation exchange column to separate threonine, serine, glycine, and alanine, wherein a column temperature when separating threonine and serine is higher than a column temperature when separating glycine and alanine.

A method for separating ionic species from an analyte solution to form a fractionated sample, the method comprising contacting the analyte solution with an ion-exchanger that is selectively permeable to ionic species of either a positive or negative charge, contacting an opposing side of the ion-exchanger with a draw solution, wherein the draw solution comprises adsorber particles dispersed in a liquid carrier, establishing a concentration gradient across the ion-exchanger to allow at least some ionic species from the analyte solution to permeate through the ion-exchanger to the draw solution, adsorbing ionic species that permeate from the analyte solution onto the adsorber particles, separating adsorber particles having the ionic species adsorbed thereto from at least part of the draw solution, and eluting the ionic species from the separated adsorber particles to form a fractionated analyte sample comprising eluted ionic species.

A method for separating ionic species from an analyte solution to form a fractionated sample, the method comprising contacting the analyte solution with an ion-exchanger that is selectively permeable to ionic species of either a positive or negative charge, contacting an opposing side of the ion-exchanger with a draw solution, wherein the draw solution comprises adsorber particles dispersed in a liquid carrier, establishing a concentration gradient across the ion-exchanger to allow at least some ionic species from the analyte solution to permeate through the ion-exchanger to the draw solution, adsorbing ionic species that permeate from the analyte solution onto the adsorber particles, separating adsorber particles having the ionic species adsorbed thereto from at least part of the draw solution, and eluting the ionic species from the separated adsorber particles to form a fractionated analyte sample comprising eluted ionic species.

The invention relates to a method for obtaining an N-acetylglucosamine containing neutral oligosaccharide from a fermentation broth, wherein said oligosaccharide is produced by culturing a genetically modified microorganism capable of producing said oligosaccharide from an internalized carbohydrate precursor, comprising the steps of: i) ultrafiltration (UF), preferably to separate biomass from the broth, ii) nanofiltration (NF), preferably to concentrate said oligosaccharide in the broth and/or reduce an inorganic salt content of the broth, and iii) treating the broth with an ion exchange resin, preferably to remove charged materials, and/or subjecting the broth to chromatography, preferably to remove hydrophobic impurities.

The invention relates to a method for obtaining an N-acetylglucosamine containing neutral oligosaccharide from a fermentation broth, wherein said oligosaccharide is produced by culturing a genetically modified microorganism capable of producing said oligosaccharide from an internalized carbohydrate precursor, comprising the steps of: i) ultrafiltration (UF), preferably to separate biomass from the broth, ii) nanofiltration (NF), preferably to concentrate said oligosaccharide in the broth and/or reduce an inorganic salt content of the broth, and iii) treating the broth with an ion exchange resin, preferably to remove charged materials, and/or subjecting the broth to chromatography, preferably to remove hydrophobic impurities.