The present invention generally pertains to methods of characterizing antibodies and related products. In particular, the present invention pertains to the use of immunoprecipitation and native strong cation exchange chromatography-mass spectrometry to specifically and sensitively detected and quantitate antibodies and related products in a sample.

The present invention generally pertains to methods of characterizing charge variants of a protein. In particular, the present invention pertains to the use of anion exchange chromatography-mass spectrometry (AEX-MS) methods using a salt-gradient. The present invention is particularly useful for charge variant analysis of IgG4 subclasses.

An electrolytic eluent generator includes an electrolyte reservoir and at least one eluent generation cartridge. The electrolyte reservoir includes a chamber containing an aqueous electrolyte solution; and a first electrode. The at least one eluent generation cartridge includes a platinum mesh electrode; a polymer screen; a plurality of reinforced membranes; a membrane washer; and a spacer including a central post and an annular projection.

The present invention relates to a method for increasing antibody yield during antibody purification from a sample by ion exchange chromatography in flow-through mode by pre-conditioning the sample with Tris without the use of NaCl to adjust the conductivity.

The present invention relates to a method for increasing antibody yield during antibody purification from a sample by ion exchange chromatography in flow-through mode by pre-conditioning the sample with Tris without the use of NaCl to adjust the conductivity.

An ion suppressor includes ion exchange membranes between a pair of electrodes. Regeneration liquid channels are provided in the spaces between the electrodes and the ion exchange membranes, and an eluent channel is provided between the ion exchange membranes. Ion re-exchange in the eluent on the downstream side of the eluent channel is suppressed, thereby making it possible to improve the detection sensitivity for the ion to be measured. For example, the eluent channel has a folded structure, thereby increasing the amount of current on the downstream side of the eluent channel, and thus, the accumulation of ions is suppressed, and accordingly, ion re-exchange in the eluent can be suppressed.

An ion suppressor includes ion exchange membranes between a pair of electrodes. Regeneration liquid channels are provided in the spaces between the electrodes and the ion exchange membranes, and an eluent channel is provided between the ion exchange membranes. Ion re-exchange in the eluent on the downstream side of the eluent channel is suppressed, thereby making it possible to improve the detection sensitivity for the ion to be measured. For example, the eluent channel has a folded structure, thereby increasing the amount of current on the downstream side of the eluent channel, and thus, the accumulation of ions is suppressed, and accordingly, ion re-exchange in the eluent can be suppressed.

Functionalized particulate support material and chromatographic media prepared therefrom are disclosed. The functionalized particulate support material is a plurality of particles, each particle having a particle surface. Chemically bonded to and extending from the surface of the particles is a combination of hydrophobic and hydrophilic functional groups. The hydrophobic functional groups enable polymerization of one or more monomers onto the particle surface while the hydrophilic functional groups provide increased wettability of the particle surface compared to an unmodified particle surface. The functionalized particulate support material may be further processed so as to form polymer chains extending from the hydrophobic functional groups. In one embodiment, the resulting polymer functionalized material is useful as a chromatographic media in chromatography columns or cartridges, such as in a liquid chromatography (HPLC) column. Chromatography columns or cartridges containing the polymer functionalized media, and methods of making and using the media, are also disclosed.

Functionalized particulate support material and chromatographic media prepared therefrom are disclosed. The functionalized particulate support material is a plurality of particles, each particle having a particle surface. Chemically bonded to and extending from the surface of the particles is a combination of hydrophobic and hydrophilic functional groups. The hydrophobic functional groups enable polymerization of one or more monomers onto the particle surface while the hydrophilic functional groups provide increased wettability of the particle surface compared to an unmodified particle surface. The functionalized particulate support material may be further processed so as to form polymer chains extending from the hydrophobic functional groups. In one embodiment, the resulting polymer functionalized material is useful as a chromatographic media in chromatography columns or cartridges, such as in a liquid chromatography (HPLC) column. Chromatography columns or cartridges containing the polymer functionalized media, and methods of making and using the media, are also disclosed.

An ion suppressor includes ion exchange membranes between a pair of electrodes. Regeneration liquid channels are provided in the spaces between the electrodes and the ion exchange membranes, and an eluent channel is provided between the ion exchange membranes. In the space between the electrode and the eluent channel, an element that increases the resistance in the voltage application direction is disposed. For example, ion permeable membranes are disposed in contact with the ion exchange membrane, thereby increasing the resistance in the voltage application direction.