A liquid handling device includes a common channel, a plurality of wells, a magnetic beads chamber and a plurality of valves. The plurality of valves are rotary membrane valves disposed on the circumference of a first circle. The magnetic beads chamber is disposed on a circumference of the second circle concentric with the first circle.

The present invention relates to a micro-object extraction method using diffusiophoresis enabling collection and extraction of micro-objects by using the concentration difference of a solution including the micro-objects to be extracted, and a micro-object identification method using same, wherein the present invention has the following advantages: desired micro-objects can be easily extracted only with a simple device by using diffusiophoresis; the collection and extraction of micro-objects can be easily controlled by changing the type of solution injected into a micro-channel; and energy usage is efficient by using self-powered energy by diffusiophoresis without separate external power required for extracting micro-objects.

A fluid device includes a substrate and a gas-liquid separating filter, the substrate has a flow path through which a solution flows, a reservoir, in which the solution is accommodated, connected to the flow path, an injection hole configured to connect the reservoir to the outside, and an air introduction hole branched off from the injection hole and connected to the outside, and the gas-liquid separating filter is disposed in a path of the air introduction hole, allows passage of a gas flowing through the air introduction hole, and prevents passage of a liquid flowing through the air introduction hole.

Provided are a flow cell and an automatic analysis device which are excellent in the long-term stability of the flow path shape of the flow cell and can improve the reproducibility of analysis. The flow cell (6) of the present invention includes a base (18) including an inlet and outlet (35, 36) for a flow path of a reaction solution containing an analyte; an electrode (16A, 16B) for applying a voltage to the analyte; a light receiving window (22) made of a member that transmits light emitted from the analyte by the voltage applied on the electrode, a gasket (18A) provided between the base (18) and the light receiving window (22), and a flow chamber (17) surrounded by the base (18), the light receiving window (22) and the gasket (18A), in which the gasket (18A) has a deformation amount of 0 mm or more and 0.2 mm or less in a chemical immersion test, and the surface adhesive force of 14 N/cm.sup.2 or more and 40 N/cm.sup.2 or less in a surface adhesion evaluation

Provided are a flow cell and an automatic analysis device which are excellent in the long-term stability of the flow path shape of the flow cell and can improve the reproducibility of analysis. The flow cell (6) of the present invention includes a base (18) including an inlet and outlet (35, 36) for a flow path of a reaction solution containing an analyte; an electrode (16A, 16B) for applying a voltage to the analyte; a light receiving window (22) made of a member that transmits light emitted from the analyte by the voltage applied on the electrode, a gasket (18A) provided between the base (18) and the light receiving window (22), and a flow chamber (17) surrounded by the base (18), the light receiving window (22) and the gasket (18A), in which the gasket (18A) has a deformation amount of 0 mm or more and 0.2 mm or less in a chemical immersion test, and the surface adhesive force of 14 N/cm.sup.2 or more and 40 N/cm.sup.2 or less in a surface adhesion evaluation

There is provided a nucleic acid extraction method using a cartridge comprising: (a) a driving part of a nucleic acid extraction device is connected to a control rod module disposed in an inner space of the upper body of a piston and a rotation control module coupled to the lower body of the piston; (b) driving the rotation control module and the control rod module, sequentially sucking sample and reagents from the plurality of chambers separated from each other into an interior space, and discharging the mixture of the interior space into the chamber of the cartridge; and (c) driving the rotation control module and the control rod module to suck the reagent inside the master mix bead chamber of the cartridge into the interior space of the piston upper body and then discharge the mixed reagent to a nucleic acid amplification module.

There is provided a nucleic acid extraction method using a cartridge comprising: (a) a driving part of a nucleic acid extraction device is connected to a control rod module disposed in an inner space of the upper body of a piston and a rotation control module coupled to the lower body of the piston; (b) driving the rotation control module and the control rod module, sequentially sucking sample and reagents from the plurality of chambers separated from each other into an interior space, and discharging the mixture of the interior space into the chamber of the cartridge; and (c) driving the rotation control module and the control rod module to suck the reagent inside the master mix bead chamber of the cartridge into the interior space of the piston upper body and then discharge the mixed reagent to a nucleic acid amplification module.

The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.

The present disclosure relates to a sensor device, comprising: a measurement chamber having at least a first wall, the measurement chamber including a plurality of analyte sensors; wherein the measurement chamber allows a fluid to be analyzed to interact with each of the plurality of analyte sensors when the fluid is accommodated within the measurement chamber; the measurement chamber having an inlet configured to receive the fluid to be analyzed and an outlet configured to allow the fluid to exit the measurement chamber after having interacted with the plurality of analyte sensors; the measurement chamber defining a sample volume for accommodating the fluid to be analyzed, the sample volume extending at least between the inlet and the outlet; a heating element configured to heat the fluid accommodated within the measurement chamber.

This invention provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some cases, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.