A method for determining whether a test sample contains a phytopathogenic fungus, which includes (a) putting the test sample on a front surface of a substrate having a through hole; the substrate having a cellulose film on the back surface thereof; the through hole has a cross-sectional area of not less than 7.065 square micrometers and not more than 19.625 square micrometers; and the cellulose film has a thickness of not less than 0.5 micrometers and not more than 3.7 micrometers; (b) leaving the test sample at rest after the step (a); (c) irradiating the cellulose film with ultraviolet light after the step (b); (d) bringing a back surface of the cellulose film into contact with a fungus color reaction reagent after the step (c); and (e) determining that the test sample contains the phytopathogenic fungus, if a color is given to the fungus color reaction reagent.

Materials and methods for detecting and treating Coccidioidomycosis (Valley Fever) are provided herein. For example, materials and methods for enriching and detecting biomarker antigens (e.g., polypeptides and/or glycans) from Coccidioides immitis and Coccidioides posadasii, the fungi that cause Valley Fever, are described herein, as are methods for treating an individual for Valley Fever based on the results of the described detection methods.

The present invention provides a method for determining whether or not a test sample contains a phytopathogenic fungi selectively from two kinds of fungi of a phytopathogenic fungus and a non-phytopathogenic fungus. The method according to the present invention comprises: (a) putting the test sample on a front surface of a substrate comprising a through hole; wherein the substrate comprises a cellulose film on the back surface thereof; the cellulose film has a thickness of not less than 0.5 micrometers and not more than 2 micrometers; and the through hole has a cross-sectional area of not less than 7.065 square micrometers and not more than 19.625 square micrometers; (b) leaving the test sample at rest; (c) observing a back surface of the film; and (d) determining that the test sample contains the phytopathogenic fungus, if a fungus is found on the back surface of the film.

A method for determining whether a test sample contains a phytopathogenic fungus, which includes (a) putting the test sample on a front surface of a substrate having a through hole; the substrate having a cellulose film on the back surface thereof; the through hole has a cross-sectional area of not less than 7.065 square micrometers and not more than 19.625 square micrometers; and the cellulose film has a thickness of not less than 0.5 micrometers and not more than 3.7 micrometers; (b) leaving the test sample at rest after the step (a); (c) irradiating the cellulose film with ultraviolet light after the step (b); (d) bringing a back surface of the cellulose film into contact with a fungus color reaction reagent after the step (c); and (e) determining that the test sample contains the phytopathogenic fungus, if a color is given to the fungus color reaction reagent.

The present invention provides a method for determining whether or not a test sample contains a phytopathogenic fungi selectively from two kinds of fungi of a phytopathogenic fungus and a non-phytopathogenic fungus. The method according to the present invention comprises: (a) putting the test sample on a front surface of a substrate comprising a through hole; wherein the substrate comprises a cellulose film on the back surface thereof; the cellulose film has a thickness of not less than 0.5 micrometers and not more than 2 micrometers; and the through hole has a cross-sectional area of not less than 7.065 square micrometers and not more than 19.625 square micrometers; (b) leaving the test sample at rest; (c) observing a back surface of the film; and (d) determining that the test sample contains the phytopathogenic fungus, if a fungus is found on the back surface of the film.

A method and system for detecting Fusarium moniliforme species of rice seeds are provided, relating to the field of rapid quality detection of rice seeds. The method includes: inputting a hyperspectral image of to-be-tested rice seeds to a model for detecting Fusarium moniliforme species of rice seed, to determine a test result of the rice seeds, where the test result is no Fusarium moniliforme or a Fusarium species. The model for detecting Fusarium moniliforme species of rice seed is determined based on activated wavelengths and an original deep convolutional neural network; the activated wavelengths are wavelengths activated by a trained deep convolutional neural network upon correct classification; and the trained deep convolutional neural network is a neural network obtained by training the original deep convolutional neural network based on the training set.

A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, deoxynivalenol and T-2 toxin. The kit includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing freeze-dried products of europium-labeled monoclonal antibodies of toxins, where the immunochromatography time-resolved fluorescence test strip includes a liner, where a water absorption pad, a detection pad and a sample pad are sequentially attached to one side of the liner from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a transverse quality control line and detection lines are arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, the three detection lines are located below the quality control line, and the detection lines each are coated with a toxin-protein conjugate.

Amatoxins (AMAs) are lethal toxins found in a variety of mushroom species. Detection methods are needed to determine the occurrence of AMAs in mushroom species, often suspected in mushroom poisonings. Provided herein are novel, sensitive monoclonal antibodies (mAbs) detection and purification techniques utilizing the mAbs that show selectivity for α-amanitin (α-AMA), β-amanitin (β-AMA) and γ-amanitin (γ-AMA).

The present application provides a fungal (1,3)-Beta-D glucan-directed monoclonal antibody, coding genes thereof, expression thereof and application thereof and belongs to the technical area of medicine & biomedical detection. The antibody contains complementarity determining regions of a light chain variable region and its amino acid sequences contain: VL-CDR1 shown by SEQ ID NO:1, VL-CDR2 shown by SEQ ID NO:2 and VL-CDR3 shown by SEQ ID NO:3; the antibody also contains cornplementarity determining regions of a heavy chain variable region and its amino acid sequences contain: VH-CDR1 shown by SEQ ID NO:4, VH-CDR2 shown by SEQ ID NO:5 and VH-CDR3 shown by SEQ ID NO:6. This antibody specifically binds with the fungal (1,3)-Beta-D glucan, has strong antibody affinity and does not trigger cross reaction with interference substances.

Portable real-time airborne fungi acquiring and detecting equipment and method are provided, the equipment includes a light source device, a manual constant-flow air pump, an impactor, an airborne fungi enrichment and dyeing device, and a fluorescence data collecting and processing device sequentially connected. The fluorescence detection technology is combined with the microparticle separation technology to develop the portable airborne fungi real-time acquiring and detecting equipment. This equipment improves the complex and extensive collection methods in conventional airborne fungi detection and the demand limitation of independent detection equipment, and realizes the real-time collection and quantification of airborne fungi concentration. Moreover, the equipment has the advantages of small volume, low costs, easy operation and is easy to be prompted.