A method for synthesis, assembly and function test of an artificial chloroplast genome of Chlamydomonas reinhardtii. Rational design has been carried out on the Chlamydomonas reinhardtii chloroplast genome for the first time, and total artificial synthesis of the Chlamydomonas reinhardtii chloroplast genome is proposed. By using totally chemically synthesized chloroplast genome segments, total chemical de novo synthesis and assembly of a chloroplast genome are achieved in a yeast-bacterium system. Then, a totally chemically synthesized chloroplast genome is transformed into Chlamydomonas cells to replace the original chloroplast genome, which works normally, and has been verified, fulfilling biological functions of the totally chemically synthesized chloroplast genome. According to the embodiments, the Chlamydomonas reinhardtii chloroplast genome is an efficient platform for carrying out synthetic biology operation.

A method for extracting an analyte in a sample is described. A sample and a solution in a microwave-extraction vial are microwave-heated and agitated. A vapor produced in the vial can be extracted into a liquid-phase medium contained in a porous membrane bag situated in the vial. The liquid-phase medium containing the vapor extract may then be analyzed for an analyte with gas chromatography-mass spectrometry.

An object of the present disclosure is to provide a technique for more efficiently obtaining a photosynthetic dye-containing cell having a desired characteristic.

The present disclosure is directed to providing a method for sorting a photosynthetic dye-containing cell, including a measurement step of measuring autofluorescence spectra of the cultured photosynthetic dye-containing cell group; and a sorting step of sorting a cell population having a suppressed photosynthetic-dye decrease from the photosynthetic dye-containing cell group on the basis of the measured autofluorescence spectra. The present disclosure is also directed to providing a cell sorter and a cell sorting system configured to perform the sorting method.

The present invention relates to a detection method for detecting the presence or absence of a test substance in a sample, the detection method including a measurement step of measuring a delayed luminescence A of a measurement object obtained by bringing dried algae into contact with the sample.

The present invention relates to a vegan immunoassay for detecting a biologically active antigen, a device in which such an immunoassay is arranged, a kit comprising such a device as well as the use of the immunoassay and a method for detecting a biologically active antigen.

In one embodiment, a fluorescence-based kit for detection and analysis of a toxin in a liquid sample includes a diluted antibody mixture in a preparation container to receive the liquid sample to produce a preparation solution; a fluorescent compound absorbed onto colloidal graphene in an analysis container to receive the preparation solution to produce an analysis solution; and a portable detector including one or more reference solutions each having a different one of a plurality of known levels of toxin concentration of the toxin, and a light source to shine a light onto the analysis solution and the one or more reference solutions.