The present invention relates to a kit for quantitative analysis of glycated hemoglobin (HbA1c), and the kit for quantitative analysis of HbA1c according to the present invention has excellent long-term stability of an enzyme reagent and thus has an effect of easily overcoming the disadvantages of the conventional reagents used in enzyme assays (e.g., storage, accuracy, portability, convenience of use, etc.).

Contemplated compositions and methods are directed to prevent and/or treat various autoimmune diseases that are typically associated with glycan dysregulation, and especially autoimmune demyelinating diseases. Further especially contemplated aspects include animal models and systems for screening compounds to treat and/or prevent such diseases.

There is provided a method of detecting in a sample the presence of an anti-M and/or anti-A and/or anti-C/Y antibody, the method comprising contacting the sample with a diagnostic conjugate provided according to the invention, comprising an oligosaccharide which comprises at least two units of 4,6-dideoxy-4-acylamido-α-pyranose and comprising at least one -(1-3)-link between adjacent 4,6-dideoxy-4-acylamido-α-pyranose units, in which the carbon at position 5 in the pyranose is linked to an R group, where R is independently selected from —CH.sub.2OH, —H or an alkyl group having at least one C atom, the oligosaccharide being covalently linked to a non-saccharide molecule or to a surface.

The invention provides methods of treating α-galactosidase A deficiency. Dosage forms, methods of administration, and methods of analyzing human α-galactosidase A are also included.

The invention consists of a method for determining Total Dietary Fiber (TDF) and its sub-fractions, Insoluble Dietary Fiber (IDF) and Soluble Dietary Fiber (SDF) in food and feed samples which utilizes flexible reaction/filtration containers that can be divided into one or more sections for capturing the IDF and SDF fractions separately or for capturing TDF in its entirety. Each container is fashioned as a bag that can be temporarily sealed in multiple locations to create multiple sections and is made of non-porous and porous material. Use of these containers eliminates the need for problematic transfers of mixtures from beaker to filter, and vastly improves the filtration process.

Methods for derivatization of biomolecules including glycans or other biopolymers with one or more fluorescent, MS active compounds by reductive amination or rapid tagging in order to produce derivatized glycan having a pKa>7 and between about 200 Å.sup.2 and about 1000 Å.sup.2 of nonpolar surface area are described.

A living plant can function as self-powered auto-samplers and preconcentrators of an analyte within ambient groundwater, detectors of the analyte contained therein. For example, a pair of near infrared (IR) fluorescent sensors embedded within the mesophyll of the plant leaf can be used as detectors of the nitroaromatic molecules, with the first IR channel engineered through CoPhMoRe to recognize analyte via an IR fluorescent emission and the second IR channel including a functionalized nanostructure that acts as an invariant reference signal.

Provided is a more accurate method for determining the possibility that a subject has developed IgA nephropathy. A method of determining the possibility that a subject has developed IgA nephropathy, in accordance with an aspect of the present invention, includes the step of determining the level of at least one glycan in a sample taken from the subject, the at least one glycan being at least one glycan that binds to at least one lectin selected from the group consisting of ACA, MAH, ABA, STL, LEL, WGA, MPA, Jacalin, MAL_I, PNA, ACG, GSL_I_A4, ConA, SSA, AOL, and GSL_II.

The present disclosure provides new reagents for labeling and detecting O-glycans released from a glycoconjugate, such as a glycoprotein, glycopeptide, peptidoglycan, or proteoglycan of interest. O-glycans labeled with the labels can be detected by fluorescence, MS, and UV. The invention further provides methods for making the reagents, methods for using them, and kits comprising them. O-glycans labeled with the reagents can be analyzed by high-throughput analysis.

Provided herein are methods for assessing cell surface glycans, e.g., N-glycans, by assessing a sample of released surface glycans, and determining the presence, absence, or level of glycans present in the sample. Also provided are methods of assaying and/or evaluating a cell composition by assessing the cell surface glycan profile of the cell composition and comparing the profile to a reference sample. Methods for manufacturing and/or culturing a plurality of cell compositions having consistent surface glycan expression with low variability are also provided.