Methods and systems for controlling a polymerization reactor in a non-sticking regime are disclosed. An exemplary method includes measuring parameters for the polymerization reaction including a reactor temperature and a concentration of an induced condensing agent (ICA) in a polymerization reactor. An equivalent partial pressure ((PICA)equiv) of the ICA is calculated. The polymerization reactor operation is located in a two dimension space defined by a reactor temperature dimension and a ((PICA)equiv) dimension. The location in the two dimensional space is compared to an non-sticking regime, defined as the space between an upper temperature limit (UTL) curve and a lower temperature limit (LTL) curve. Parameters of the polymerization reactor are adjusted to keep the reactor within the non-sticking regime.

An assay method for detecting antipolyvinyl alcohol (anti-PVAL) antibodies in test samples, such as serum, is described. The method comprises combining a sample to be tested for an anti-PVAL antibody with polyvinyl alcohol (PVAL) to form a binary complex of the PVAL with an anti-PVAL antibody in the sample, followed by reacting an indicator reagent with the binary complex to form a labeled ternary complex, and then detecting the presence or absence of the ternary complex in the sample. Kits for performing the test are also described.

The present invention relates to a method for determining the amount of a physiologically acceptable polymer molecule bound to a protein, an antibody or other composition being capable of specifically binding to a physiologically acceptable polymer molecule, and a kit containing said antibody or composition.

The present invention relates to a method for determining the amount of a physiologically acceptable polymer molecule bound to a protein, an antibody or other composition being capable of specifically binding to a physiologically acceptable polymer molecule, and a kit containing said antibody or composition.

The present invention relates to methods of detecting off-target binding of proteins such as antibodies. Said methods are useful in identifying proteins that are likely to have more rapid clearance in vivo compared to proteins that do not have (or they have less) off-target binding.

Disclosed herein are aspects of a composition for detecting environmental toxicants such as, but not limited to, perfluorinated-alkyl substance, perfluorooctanoic acid, and the like. The composition allows for the rapid, sensitive detection of perfluorinated-alkyl substances (PFAS) by utilizing the competitive interactions during capillary action. The composition comprising one or more cellulose fibers; and one or more biomolecules associated with the one or more cellulose fibers, wherein the composition is configured so that when the composition contacts the liquid sample, the one or more biomolecules disassociate from the one or more cellulose fibers by interacting with the one or more environmental toxicants thereby decreasing the flow of the liquid sample comprising one or more environmental toxicants. A system for using the composition is also disclosed along with a method of using the paper microfluidic chips and a method making the paper microfluidic chip.

The present disclosure relates generally to a method for identifying at least one microplastic in a protein-containing liquid biological sample, wherein the method comprises: heating the protein-containing liquid biological sample to provide a protein-containing sediment and a liquid phase disposed above the sediment; separating the liquid phase from the protein-containing sediment; evaporating the liquid phase to provide an analyte; treating the analyte with a depolymerization solution comprising an organic phase and an aqueous phase; extracting the analyte from the depolymerization solution in the organic phase of the depolymerization solution; filtering the organic phase to provide an analyte residue as the oversize; and identifying the at least one microplastic in the analyte residue with size-exclusion liquid chromatography and mass spectrometry.