Immobilization and magnetic extraction of pathogens and pathogen components The application describes a method for reducing the concentration of pathogens and/or pathogen components in an aqueous or body fluid sample. Specifically, the method relates to incubating the sample with superparamagnetic iron-based particles attached to a target binding peptide and immobilising the superparamagnetic iron-based particles with a magnetic field and thereby separating the pathogen-bound and/or pathogen component-bound superparamagnetic iron-based particles from the sample. Furthermore, the application relates to a method for identifying pathogens in an aqueous or body fluid sample a use of superparamagnetic iron-based particles for reducing the concentration of pathogens and/or pathogen components in an aqueous or body fluid sample. In addition, a use of superparamagnetic iron-based particles for identifying pathogens in an aqueous or body fluid sample is disclosed. Finally, superparamagnetic ironoxide nanoparticles (SPION's) are disclosed, wherein the SPIONs are linked to a target binding peptide. wherein the target is a pathogen, and/or a pathogen component.

The invention relates to the use of the monoclonal antibody NILO1 for the diagnosis, treatment and/or prevention of brain tumors and lesions. Particularly, the invention relates to methods for the diagnosis of brain tumors and brain lesions in which cells marked with said antibody, or with immunologically active fragments thereof, are detected. The invention also relates to the use of said monoclonal antibody, or immunologically active fragments thereof, as a medicament for the treatment and/or prevention of brain tumors and brain lesions. In a preferred embodiment of the invention, the monoclonal antibody NILO1, or its immunologically active fragments, are humanized.

A multiplex hand-held diagnostic biosensor, using two inflammatory salivary biomarkers, Human Neutrophil Elastase (HNE) and Cathepsin-G, was constructed made to potentially detect Periodontitis at an early stage is described. The use of magnetic nanoparticle biosensor method used as a device was based on the measurement of proteolytic activity using specific proteases probes. The magnetic nanoparticle biosensor device is capable of specific and quantitative detection of HNE and Cathepsin-G in solution and in spiked saliva samples with a lower detection limit of 1 pg/mL and 100 fg/mL for HNE and Cathepsin-G, respectively.

Provided herein are methods of assessing or determining the presence or absence of particles, such as bead particles, present in a cell composition. Also provided are articles of manufacture and kits for use in the methods.

Described herein are virus-like particles (VLPs) that were generated to present the viral glycoprotein antigens on their surface, coupled to magnetic fluorescent microspheres to create VLP-conjugated microspheres (VCMs) and methods of making them. These VCMs were stable when lyophilized and stored at 37 C. and able to detect antibodies in non-human primate (NHP) and human clinical sera at dilutions of 110.sup.5 and 110.sup.4, respectively. Methods are also disclosed for detecting an immune response using VCMs.

The present invention relates to microparticles for analyzing biomolecules, a biomolecule analysis kit comprising the microparticles, and a method for analyzing biomolecules using the analysis kit, the microparticles for analyzing biomolecules comprising: a core including at least one selected from among an optical expression substance, a metallic material, and a magnetic material; a silica coating layer formed on the core; and at least one binding means, linked to the silica coating layer, for binding to an analysis subject biomolecule, wherein the optical expression substance is a fluorescent or a luminescent.

Disclosed are a metal nanoparticle-magnetic particle complex, comprising a core comprising a magnetic particle, the first shell comprising metal nanoparticles and formed on the surface of the core, and the second shell comprising a response factor and formed on the surface of the first shell, a method of preparing the complex, and a method of measuring the concentration of a biomarker in a sample using the complex.

Disclosed herein is an integrated microfluidic chip for detecting cancerous cells, particularly, cholangio-cancerous cells, from a biological sample. Also disclosed herein is a method of detecting cholangio-cancerous cells from a biological sample.

A solid-phase support, formed by binding a chain polymer at least to the surface, wherein the chain polymer is a block polymer comprising a first block constituted from repetitions of a hydrophilic structural unit and a second block constituted from repetitions of a structural unit having a reactive functional group, a content ratio of the number of moles a of the reactive functional group contained in the chain polymer and the number of moles b of the whole structural unit contained in the chain polymer, (a/b), is from 0.03 to 0.25, and a density of the chain polymer occupying the surface of the solid-phase support is 0.1 polymers/nm.sup.2 or more.

The invention generally relates to using magnetic particles and magnets to isolate a target analyte from a body fluid sample. In certain embodiments, methods of the invention involve introducing magnetic particles including a target-specific binding moiety to a body fluid sample in order to create a mixture, incubating the mixture to allow the particles to bind to a target, applying a magnetic field to capture target/magnetic particle complexes on a surface, and washing with a wash solution that reduces particle aggregation, thereby isolating target/magnetic particle complexes.