Provided herein are methods for simultaneous spatio-temporal measurement of gene expression and cellular activity.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The invention relates to methods and kits for assessing brain injury, e.g., traumatic brain injury resulting from blast exposure. The invention provides methods of quantifying the amount of phosphorylated tau or total tau in a biological sample. The invention further provides a method of determining the number of blast exposures experienced by a subject. Also provided herein are kits for detecting phosphorylated tau or total tau in a biological sample.

A connector is provided for analysing biological samples including at least one first affinity reagent configured to bind directly or indirectly to a target molecule, and a backbone connected to the first affinity reagent and having at least one first affinity interactor, wherein the first affinity interactor is configured to specifically bind to a second affinity interactor having a label in order to bind the label to the backbone, and wherein the backbone has a cleavage site for irreversibly separating the first affinity reagent and the first affinity interactor. In a further aspect, a marker and a method for analysing biological samples are provided.

The present invention relates to an in vitro method for determining the presence, absence and/or concentration of an analyte in a sample. The method uses an optically based competition assay comprising a labelled analyte binding protein and a labelled analyte analogue. The concentration/presence of the analyte is determined by inhibitory binding of the analyte to the analyte binding protein thereby impeding binding of the analyte analogue to the analyte binding protein. The invention further relates to kits, solid supports, cartridges, detection chips and uses thereof.

A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements (including an integrated imaging subsystem); a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including antibody detection, other protein detection, mRNA detection, and/or other applications associated with spatial transcriptomics.

Methods of detecting a target in a sample are provided. Kits for performing the methods described herein are also provided.

The disclosure features methods that include: contacting a biological sample having a first target analyte with a first agent, where the first agent includes a first binding species that specifically binds to the first target analyte, and a first oligonucleotide conjugated to the binding species; contacting the biological sample with a second agent, where the second agent includes a first reactive species and a second oligonucleotide conjugated to the first reactive species, to hybridize at least a portion of the second oligonucleotide to at least a portion of the first oligonucleotide; and contacting the biological sample with a first labeling species, where the first labeling species reacts with the first reactive species to deposit the first labeling species or a derivative thereof in the biological sample.

An object of the present technology is to improve analysis efficiency in single cell analysis using a barcoding technique.

The present technology provides a bioparticle analysis method including: a capture step of capturing a bioparticle on a surface, on which a molecule including a bioparticle capturing part, a barcode sequence, and a cleavable linker is immobilized via the linker, via the bioparticle capturing part; a cleavage step of cleaving the linker to release the bioparticle from the surface; and an isolation step of isolating the bioparticle into a microspace. Furthermore, the present technology also provides a bioparticle analysis system that performs the bioparticle analysis method.