Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. In some embodiments, the reader component communicates with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

The invention generally relates to materials and methods for creating and/or utilizing upconverting luminescence. More particularly, the invention relates to novel compositions (e.g., nanoparticles) and related methods of preparation and use that enable upconverting luminescence with an efficient excitation optimized at about 800 nm. A unique class of cascade sensitized tri-doped UCNPs with a biocompatiable 800 nm excitable property are disclosed herein, for example, tri-doped -NaYF.sub.4:Nd, Yb, Er (Tm)/NaYF.sub.4UCNPs, which employ Nd.sup.3+ as 800 nm photon sensitizer and Yb.sup.3+ as bridging ions, having strong green or blue upconversion emissions without photobleaching.

A method and a biosensor for determination of the presence of corona-virus are provided. The method includes qualitative or quantitative measurement of probable corona-virus presence by means of colorimetric and/or electrochemical transducer methods as a result of interaction of S-protein in patient samples by means of a biosensor including ACE2, DPP4 and CD147 bio-receptors as bio-receptor.

The present invention relates to a conjugate of cell penetrating peptide and an active ingredient; and its use. Specifically, a conjugate including a cell penetrating peptide which is a peptide comprising any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156, a fragment of any one sequence of SEQ ID NO: 1 to SEQ ID NO: 156, or a peptide having above 80% homology with the above-mentioned sequence; and a composition comprising the same are disclosed.

The present disclosure provides compositions, reagents, kits, systems, system components, and methods for performing assays. More particularly, the disclosure relates to an assay composition for detecting luminescence, which comprises an alkyl diethanolamine, for example, N-butyldiethanolamine (BDEA). In emodiments, the assay composition comprises 2-dibutylaminoethanol (BDAE).

The invention relates to a method for culturing a subpopulation of circulating epithelial tumour cells from a body fluid of a human or animal suffering from an epithelial tumour, wherein cells contained in the body fluid each containing at last one cell nucleus are separated from the body fluid and cultured over at least 24 hours in suspension, with formation of spheroids.

The disclosure concerns methods for the detection of an analyte in a sample by electro-chemiluminescence using new reagent compositions. New reagent compositions, reagent kits for measuring electrochemiluminscence (ECL) and electrochemiluminescence detection methods using the new reagent compositions are disclosed. In particular, the disclosure relates to the use of novel combinations of compounds which can be used in said measurements to provide improved assay performance.

The present invention relates to a biothiol detecting composition comprising a redox regulation protein, a method for detecting biothiols by using the same, and a biosensor/kit for detecting biothiols. The present invention provides the effect of rapidly measuring free biothiols in body fluids. In addition, relative content ratios and changes of total to free biothiols in body fluids can be detected in real time, which allows biothiols to be available as main indices of diseases through which prediction and warning can be made against various diseases. Further, because various redox stress changes associated with main diseases can be accounted for by variations of biothiols, the present invention can provide important technical, economical, and social values for the investigation of pathogenesis mechanisms and the diagnosis of diseases in the future.

The present invention relates to a method for quantitating an analyte having a wide range concentration in a single assay without having to dilute the sample and repeating the assay. The key feature of the invention is having two cycles of events including sample binding to probe, binding reactions, and detection. After the first cycle of binding and detecting, the probe is dipped into the same sample vessel to bind additional analyte in the sample vessel at a condition that is more favorable to binding than the condition in the first cycle.

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.