The application relates to certain new epitope peptides associated with coronavirus envolop protein and the use thereof. In particular, the application describes the antibodies and vaccines developed against these peptides, and the use thereof for the detection and treatment of coronaviral infection in human subjects.

Embodiments may include a rapid test device that provide rapid detection of substances, including those involved in pathogen infection, for example, using Microscale Affinity Chromatography (MAC), indirect ELISA, and optical molecular sensing technology. For example, in an embodiment, a system may comprise a cartridge comprising a first chamber configured to receive a test sample including a target substance, the first chamber pre-filled with micromagnetic particles treated so as to bind to the target substance, and a first reservoir pre-filled with at least one reagent labeled with at least one fluorescent compound, the reagent adapted to bind to micromagnetic particles that are bound to the target substance, and an apparatus comprising a light source disposed so as to excite the least one fluorescent compound with a light and an optical sensor disposed so as to detect an emitted spectrum of light from the excited least one fluorescent compound.

Provided is a target substance detection method that includes: forming a complex in a first reaction field, by sandwiching a target substance between a first trapping substance immobilized on a solid phase and a second trapping substance labeled with a labeling substance; separating a moiety that includes the labeling substance from the complex; moving the moiety to a second reaction field; and detecting the target substance by a signal based on the labeling substance in the second reaction field. As a result of this technology, a target substance in a sample can be detected with greater sensitivity and at a lower cost than when using conventional technology.

The invention provides a lateral flow detection device for detecting a coronavirus by immunoassay, wherein the detection device comprises two lateral flow test strips, a test strip 1 is used to test an antibody to a N full-length protein and/or a S full-length protein, while a test strip 2 is used to test an antibody to a S-RBD site protein, and a combination of the two test strips is used to test IgG and IgM antibodies to novel coronavirus, which further improves a detection rate of serological antibodies and effectively reduces a possibility of missing detection and wrong detection, thereby avoiding any missing detection.

Systems and processes to screen for SARS-CoV-2, which includes a process that uses an alternative antibody for ELISA. The alternative antibody is an alternative to a mouse antibody, thereby expanding the ability to test for COVID-19 using non-human antibodies.

Systems and methods are disclosed. An example method of determining the concentration of at least one analyte in a plurality of samples by sequentially subjecting each sample to an analysis cycle includes contacting the sample or a sample-derived solution with a sensor surface supporting a species capable of specifically binding the analyte or an analyte-binding species, detecting the amount of binding to the sensor surface, and regenerating the sensor surface to prepare it for the next analytical cycle, and based on the detected binding to the sensor surface determining the concentration of analyte in each sample using virtual calibration data calculated for each analysis cycle from real calibration data obtained by contacting the solid phase with samples containing known concentrations of analyte.

Methods are disclosed for a sandwich assay for a small molecule having a molecular weight of about 500 to about 2,500. The method comprises the use of a first antibody that binds to the small molecule and a second antibody that binds to the small molecule at a portion of the small molecule other than a portion to which the first antibody binds. The second antibody is prepared from an immunogen that comprises a hapten that is not the small molecule or a derivative of the small molecule wherein the hapten comprises a moiety that is structurally similar to that of the second portion of the small molecule. The antibodies may be employed in sandwich assays for the small molecule.

[Problem]

To provide means for detecting heart failure more simply and highly accurately.

[Solution Means]

A method of detecting heart failure from a sample collected from an organism, the method comprising the step of: performing sandwich immunoassay of NT-proANP or a fragment thereof contained in the sample using a first antibody for capturing for which an epitope lies on any site among positions 31 to 67 of an amino acid sequence of NT-proANP and a second antibody for labeling for which an epitope lies on any site among positions 31 to 67 of the amino acid sequence of NT-proANP, and the like.

The epitopes of the two types of antibodies: one for capturing and the other for labeling, both of which lie among positions 31 to 67 of the amino acid sequence of NT-proANP. This, therefore, enables detention of a fragment having the same amount of substance as NT-proANP and simple and highly accurate detection of heart failure, even if NT-proANP is further cleaved and decomposed into 3 pieces during circulation in blood.

The present invention provides an anti-APOA4 monoclonal antibody or an antibody fragment thereof capable of accurately measuring apolipoprotein A-IV (APOA4) in a specimen, a measurement method for immunologically measuring APOA4 using the antibody or the antibody fragment thereof, and a kit for measuring APOA4 containing the antibody or the antibody fragment thereof.

A method for diagnosing tuberculosis of the present invention can improve the reactivity of tuberculous antigens and detection antibodies by increasing free tuberculous antigens in a sample through a pretreatment step in which the sample of a subject is treated with an acidic material such that the antigens are dissociated from tuberculous antigen complexes, thereby enabling a point-of-care test for tuberculosis and being immediately utilizable as a fast and simple detection method for a point-of-care test.