An optical system including a first light source, a first lens, and a light splitting module, wherein the light splitting module has a first splitter, a second lens, a first camera and a second camera, the first lens is configured for receiving a first light beam from the first light source and collimating the first light beam onto a sample, and for receiving and collimating a light beam from the sample, the second lens is configured for focusing the collimated light beam from the first lens to the first camera and the second camera, the first splitter is configured for splitting the focused light beam from the second lens into a second light beam and a third light beam, the first camera is configured for receiving the second light beam, and the second camera is configured for receiving the third light beam.

A system for a microscope includes a display and an input interface. The system is configured to reproduce, in response to a user input to the input interface, a virtual tour of use of the microscope via the display. The system can be applied, for example, for teaching of microscopy at schools and universities.

Disclosed is an apparatus and method for imaging: a side-view of a object on a surface, a Contact Angle of a liquid object, the color of an object, or combinations thereof.

An observation carrier includes a bottom base, a lower cover, an upper cover, and a rotation cover. The bottom has at least one first positioning portion. The lower cover has at least one second positioning portion, and at least one third positioning portion. The lower cover is detachably disposed on the bottom base and positioned with the first positioning portion through the second positioning portion. The upper cover has at least one fourth positioning portion and is detachably disposed on the bottom base. The upper cover is positioned with the third positioning portion through the fourth positioning portion. An observation region is formed between the upper cover and the lower cover. The rotation cover is detachably disposed on the bottom base to limit the upper and lower covers on the bottom base. The rotation cover is adapted to rotate to be locked or released by the bottom base.

An optical imaging equipment and method. The optical imaging equipment includes an optical microscope, an objective table, a light source module and an objective lens. The objective table is movable in the XY-plane, the light source module contains illumination light sources, a narrowband filters and the objective lens is movable in the Z-axis direction; a three dimensions (3D) electric sample table is fixed on the objective table, which is used for carrying a sample to be tested and driving the sample to move in 3D directions relative to the objective table; a microsphere is fixed on a transparent substrate; the objective lens, the microsphere and the sample to be tested are arranged in the Z-axis direction in sequence, wherein, the transparent substrate along with the microsphere thereon can be moved to a first position and remain stationary relative to the objective table in the Z-axis direction, the 3D electric sample table can adjust the sample to be tested with respect to the microsphere to an imaging plane which is parallel to the XY-plane and a first image is formed by the microsphere, the objective lens can be adjusted to a second position so that the objective lens can perform a secondary imaging of the first image to form a second image.

A method for aligning multiple optical components in an optical system including placing a sphere at a first position that is at a center of curvature of a first optical component, and aligning a focus of a first reference signal with the sphere at the first position. Then, moving the sphere along an axis of optical symmetry to a second position that is at a center of curvature of a second optical component, and aligning a focus of a second reference signal with the sphere at the second position. The first optical component is aligned with the first reference signal and fixing the first optical component, and the second optical component is aligned with the second reference signal and fixing the second optical component.

A photoacoustic and optical microscopy combiner. The combiner is configured to support a transducer defining an axis. The combiner includes a body including a base and an opening extending through the base, and a glass member at least partially positioned within the opening. The glass member includes a surface positioned at an angle relative to the base and the axis of the transducer. A sample slide is supported on the body and at least partially over the opening. The sample slide is positioned such that a sample on the sample slide is configured to receive light from a laser and redirect the light to an ultrasound transducer to generate a real-time image of a sample.

One aspect of this disclosure relates to a computer-implemented method for determining a force acting on at least part of a structure, for example a biological structure, such as a DNA molecule. The method comprises controlling a light-sensitive system, e.g. of a microscope, to determine light information based on light from the structure. The light is incident on at least a part of the light sensitive system. The light-sensitive system may be said to capture the light from the structure. The at least part of the structure comprises one or more optically active entities, such as DNA intercalator molecules and donor/acceptor fluorophores. At least one of (i) an optical activity of the entities and (ii) a quantity of the entities depends on the force acting on the at least part of the structure. Furthermore, the light information defines a light property value associated with said at least part of the structure. The method further comprises determining the force acting on the at least part of the structure on the basis of said light property value and a reference light property value.

The present invention relates to a method of identifying or monitoring circulatory failure in a subject, which method comprises assessing the subject's microcirculation in respect of the following parameters: (a) functional capillary density (FCD); (b) heterogeneity of the FCD; (c) capillary flow velocity; (d) heterogeneity of capillary flow velocity; (e) oxygen saturation of microvascular erythrocytes (SmvO.sub.2); and (f) heterogeneity of SmvO.sub.2; wherein parameters (a) to (d) are assessed visually by microscopy and parameters (e) and (f) are assessed by diffuse reflectance spectroscopy (DRS); as well as apparatus and software designed for performance of such a method.

The present disclosure presents systems, apparatuses, and methods of holographic imaging. In this regard, a method comprises transmitting light and illuminating a semi-transparent sample object; and forming, at a hologram plane, an interference pattern of a real image of the sample object from a scattered object beam and an unscattered reference beam from the transmitted light. To do so, the scattered object beam and the unscattered reference beam are in-line with one another, and a distance between the hologram plane to the sample object is set at a distance that substantially weakens a virtual image of the sample object formed from the scattered object beam and the unscattered reference beam. Accordingly, the method further comprises recording the interference pattern of a hologram formed from the scattered object beam and the unscattered reference beam at a detector; and reconstructing a 3D optical field of the hologram without phase retrieval.