A method of automated measurement of motility and morphology parameters of the same single motile sperm. Automated motility and morphology measurements of the same single sperm are performed under different microscope magnifications. The same single motile sperm is automatically positioned and kept inside microscope field of view and in focus after magnification switch. A method of automated non-invasive measurement of sperm morphology parameters under high magnification of imaging. Sperm morphology parameters including subcellular structures are automatically measured without invasive sample staining. A method of automatically selecting sperms with normal motility and morphology and DNA integrity for infertility treatment.

Deterioration of optical characteristics is suppressed. An optical measurement device according to an embodiment includes: an excitation light source (101 to 103) that emits excitation light having a wavelength of at least 450 nanometers or less; a lens structure (116) that condenses the excitation light at a predetermined position; a fluorescence detection system (140) that detects fluorescence emitted from a particle by excitation of the particle present at the predetermined position by the excitation light; and a scattered light detection system (130) that detects scattered light generated by the excitation light being scattered by the particle present at the predetermined position, and the lens structure includes a plurality of lenses (21, 22, 23, 25, 26, 28) arranged along an optical axis of the excitation light and a lens frame (10) that holds the plurality of lenses, and a position of at least one of the plurality of lenses in the lens frame is determined by abutting on a lens adjacent to the lens.

The present disclosure relates to a medical microscope field. A stereo microscope connected to an optical coherence tomography (OCT) unit for forming a tomographic image of a target object includes an objective lens unit including a plurality of lenses each having an aperture of a predetermined size, a pair of first magnification lens units each including a plurality of lenses having a pair of magnification lens apertures positioned within the aperture of the objective lens unit, a second magnification lens unit including a plurality of lenses having an OCT aperture disposed separately from the pair of magnification lens aperture within the aperture of the objective lens unit, and a light delivery unit configured to receive light from the OCT unit and deliver the light to the second magnification lens unit and configured to deliver light received from the second magnification lens unit to the OCT unit.

The objective lens unit includes, a front lens group having a negative refractive power, a diaphragm, and a rear lens group having a positive refractive power, in order from an object side. The front lens group includes a negative lens having a concave surface facing an image surface side, and a positive lens having a convex surface facing the object side, and the rear lens group includes a positive lens having a convex surface facing the image surface side and a cemented lens in which a positive lens and a negative lens are cemented. The endoscope objective lens unit satisfies −1.6<f.sub.F/f.sub.R<−1.2 and −1.0<SF.sub.5<−0.5. f.sub.F and f.sub.R are the focal lengths of the front lens group and the rear lens group, SF.sub.5 is (R.sub.51+R.sub.52)/(R.sub.51−R.sub.52), and R.sub.51 and R.sub.52 are respectively the curvature radiuses of surfaces.

A quantitative phase microscopy (QPM) system and methods are provided for sample imaging and metrology in both transmissive and reflective modes. The QPM system includes a first illuminating beam propagating along a transmission-mode path and a second illuminating beam propagating along a reflection-mode path, a microscope objective lens disposed in the reflection-mode path, and a common-path interferometer comprising a diffraction grating, a Fourier lens, a pinhole, and a 2f system lens to collimate the reference beam and the imaging beam such that the collimated reference beam and imaging beam interfere with each other to form an interferogram at a final image plane.

A quantitative phase microscopy (QPM) system and methods are provided for sample imaging and metrology in both transmissive and reflective modes. The QPM system includes a first illuminating beam propagating along a transmission-mode path and a second illuminating beam propagating along a reflection-mode path, a microscope objective lens disposed in the reflection-mode path, and a common-path interferometer comprising a diffraction grating, a Fourier lens, a pinhole, and a 2f system lens to collimate the reference beam and the imaging beam such that the collimated reference beam and imaging beam interfere with each other to form an interferogram at a final image plane.

A microscope for imaging a sample by a transmitted light contrasting method includes an objective lens holder configured to place an objective lens of a number of objective lenses onto an optical axis of the microscope. The microscope further includes a lens system for forming an intermediate image of an exit pupil of any one of the number of objective lenses placed onto the optical axis. The intermediate image is formed at a respective conjugated plane conjugate to the exit pupil. The microscope further includes a control device configured for automatically positioning a modulation element onto the optical axis at a positon related to the respective conjugated plane.

A microscope for imaging a sample by a transmitted light contrasting method includes an objective lens holder configured to place an objective lens of a number of objective lenses onto an optical axis of the microscope. The microscope further includes a lens system for forming an intermediate image of an exit pupil of any one of the number of objective lenses placed onto the optical axis. The intermediate image is formed at a respective conjugated plane conjugate to the exit pupil. The microscope further includes a control device configured for automatically positioning a modulation element onto the optical axis at a positon related to the respective conjugated plane.

Provided herein are systems and methods for imaging using a microscope system comprising removeable or replaceable component parts. Such systems and methods employ semi-kinetic coupling for easy, tool-free attachment of the microscope system to a baseplate. Systems and methods provided herein may comprise simultaneous imaging and stimulation using a microscope system. The microscope system can have a relatively small size compared to an average microscope system.

A kit (10) includes a light source (12) and an optical system (14) equipped with a lens assembly (25) defining a magnification optical axis (X-X). A frame (16) is crossable by the light generated by the light source (12). The frame (16) is configured for supporting a sample holder (H), a portable electronic apparatus (S) equipped with an image acquisition device (C), and the optical system (14), which are interposable between the sample holder (H) and the image acquisition device (C). The optical system (14) is configured for being movable in a guided manner on the frame (16), to allow aligning the optical axis (X-X) with the image acquisition device (C). A carrying body (18) is configured for receiving in abutment the frame (16) and housing the light source (12) directing light towards the optical system (14) through the frame (16).