Changing device for optical components in a microscope, comprising an optical component (12) having a flat surface (13), a carrier (1) for inserting and/or holding the optical component (12), and a receptacle (26) for holding the carrier (1) in an optical path of the microscope, characterized in that the carrier (1) has bearing surfaces (8) for the flat surface (13) of the optical component (1) and positioning surfaces (10) located in the same plane, which are not covered by the optical component (12), when inserting the latter, and the receptacle (26) has bearing surfaces (28) for contact with the positioning surfaces (10), and first attachment means (11) for attaching the carrier (1) positioned on the receptacle (26) in order for the positioning surfaces (10) to act upon the bearing surfaces (28).

Changing device for optical components in a microscope, comprising an optical component (12) having a flat surface (13), a carrier (1) for inserting and/or holding the optical component (12), and a receptacle (26) for holding the carrier (1) in an optical path of the microscope, characterized in that the carrier (1) has bearing surfaces (8) for the flat surface (13) of the optical component (1) and positioning surfaces (10) located in the same plane, which are not covered by the optical component (12), when inserting the latter, and the receptacle (26) has bearing surfaces (28) for contact with the positioning surfaces (10), and first attachment means (11) for attaching the carrier (1) positioned on the receptacle (26) in order for the positioning surfaces (10) to act upon the bearing surfaces (28).

An imaging flow cytometer includes at least one flow channel through which an observation target flows, a light source which irradiates the flow channel with sheet-like excitation light, an imaging unit which images a specific cross-section of the observation target by imaging fluorescence from the observation target having passed through a position irradiated with the excitation light, and a three-dimensional image generation unit which generates a three-dimensional image of the observation target as a captured image on the basis of a plurality of captured images obtained by cross-sectional imaging by the imaging unit.

An imaging flow cytometer includes at least one flow channel through which an observation target flows, a light source which irradiates the flow channel with sheet-like excitation light, an imaging unit which images a specific cross-section of the observation target by imaging fluorescence from the observation target having passed through a position irradiated with the excitation light, and a three-dimensional image generation unit which generates a three-dimensional image of the observation target as a captured image on the basis of a plurality of captured images obtained by cross-sectional imaging by the imaging unit.

A system and methods for ghost imaging second harmonic generation microscopy. Imaging data is collected in parallel, providing faster imagine reconstruction and enabling reconstruction in scattering environments. Ghost imaging, split light beam interacting with a target and a second light beam unimpeded and not required to pass through the same background. A second harmonic generation image is reconstructed from the detected photons.

A system and methods for ghost imaging second harmonic generation microscopy. Imaging data is collected in parallel, providing faster imagine reconstruction and enabling reconstruction in scattering environments. Ghost imaging, split light beam interacting with a target and a second light beam unimpeded and not required to pass through the same background. A second harmonic generation image is reconstructed from the detected photons.

A fluorescence microscopy inspection system includes light sources able to emit light that causes a specimen to fluoresce and light that does not cause a specimen to fluoresce. The emitted light is directed through one or more filters and objective channels towards a specimen. A ring of lights projects light at the specimen at an oblique angle through a darkfield channel. One of the filters may modify the light to match a predetermined bandgap energy associated with the specimen and another filter may filter wavelengths of light reflected from the specimen and to a camera. The camera may produce an image from the received light and specimen classification and feature analysis may be performed on the image.

A fluorescence microscopy inspection system includes light sources able to emit light that causes a specimen to fluoresce and light that does not cause a specimen to fluoresce. The emitted light is directed through one or more filters and objective channels towards a specimen. A ring of lights projects light at the specimen at an oblique angle through a darkfield channel. One of the filters may modify the light to match a predetermined bandgap energy associated with the specimen and another filter may filter wavelengths of light reflected from the specimen and to a camera. The camera may produce an image from the received light and specimen classification and feature analysis may be performed on the image.

A biological sample image collection device (100), comprising support (30) and an optical imaging assembly (50), also comprises: a plurality of movable platforms (40), for placing biological samples (20) wherein the plurality of movable platforms (40) are arranged on the support (30) in an array; the plurality of movable platforms (40) can move relative to the support (30); and forces acting on the support (30) during the movement of the movable platforms can cancel each other out, so as to avoid vibrations affecting the support (30) and the biological samples (20) are canceled. The optical imaging assembly (50) collects images of the biological samples (20) on the movable platforms (40) when the plurality of movable platforms (40) move, relative to the center of the array, in the same direction and at the same speed. Further provided is a gene sequencer including the biological sample image collection device (100).

An optical inspection system for detecting sub-micron features on a sample component. The system may have a controller, a camera responsive to the controller for capturing images, an objective lens able to capture submicron scale features on the sample component, and a pulsed light source. The pulsed light source may be used to generate light pulses. The camera may be controlled to acquire images, using the objective lens, only while the pulsed light source is providing light pulses illuminating a portion of the sample component. Relative movement between the sample component and the objective lens is provided to enable at least one of a desired subportion or an entirety of the sample component to be scanned with the camera.