The present application discloses a confocal microscope including a light generator configured to simultaneously generate reflection light, which is reflected from a sample, and transmission light, which passes through the sample; a scanner configured to optically scan the sample and define a direction of a first optical path, along which the reflection light propagates; an adjuster configured to angularly adjust a direction of a second optical path, along which the transmission light propagates; a first signal generator configured to generate a first signal based on the reflection light; a second signal generator configured to generate a second signal based on the transmission light; and an image generator configured to generate a synthetic image in which a reflection image represented by the reflection light and a transmission image represented by the transmission light are synthesized in response to the first and second signals.

Systems and methods for sensing vibrational absorption induced photothermal effect via a visible light source. A Mid-infrared photothermal probe (MI-PTP, or MIP) approach achieves 10 mM detection sensitivity and sub-micron lateral spatial resolution. Such performance exceeds the diffraction limit of infrared microscopy and allows label-free three-dimensional chemical imaging of live cells and organisms. Distributions of endogenous lipid and exogenous drug inside single cells can be visualized. MIP imaging technology may enable applications from monitoring metabolic activities to high-resolution mapping of drug molecules in living systems, which are beyond the reach of current infrared microscopy.

A microscope includes at least one illuminating lens configured to guide at least one illuminating beam in the form of a light sheet for illuminating at least one specimen to be examined. The microscope also includes at least one detection lens configured to capture at least one detection beam issuing from the at least one specimen to be examined. The at least one illuminating lens has an optical axis at an angle α, which is not equal to 90°, to an optical axis of the at least one detection lens. The at least one illuminating beam enters the at least one illuminating lens at an entry angle β such that the light sheet lies within a focal plane of the at least one detection lens.

A microscope includes a housing having a first opening and at least one second opening. A fluorescence device having exchangeable fluorescence cubes is arranged in a space enclosed by the housing. The fluorescence device is accessible through each of the first opening and the at least one second opening such that a total of at least two openings are provided, via each of which the fluorescence device is accessible and via each of which the fluorescence cubes of the fluorescence device are exchangeable.

A microscope includes a housing having a first opening and at least one second opening. A fluorescence device having exchangeable fluorescence cubes is arranged in a space enclosed by the housing. The fluorescence device is accessible through each of the first opening and the at least one second opening such that a total of at least two openings are provided, via each of which the fluorescence device is accessible and via each of which the fluorescence cubes of the fluorescence device are exchangeable.

Embodiments of a resolution enhancement technique for a light sheet microscopy system having a three objective lens arrangement in which one objective lens illuminates a sample and the second and third objective lenses collect the fluorescence emissions emitted by the sample are disclosed. The second objective lens focuses a first portion of the fluorescence emissions for detection by a second detection component, while the third objective lens focuses a second portion of the fluorescence emissions through a diffractive or refractive optic component for detection by a first detector component. A processor combines the images resulting from the first and second portions of the fluorescence emissions for generating composite images with increased axial and lateral resolution.

Embodiments of a resolution enhancement technique for a light sheet microscopy system having a three objective lens arrangement in which one objective lens illuminates a sample and the second and third objective lenses collect the fluorescence emissions emitted by the sample are disclosed. The second objective lens focuses a first portion of the fluorescence emissions for detection by a second detection component, while the third objective lens focuses a second portion of the fluorescence emissions through a diffractive or refractive optic component for detection by a first detector component. A processor combines the images resulting from the first and second portions of the fluorescence emissions for generating composite images with increased axial and lateral resolution.

Systems and methods for sensing vibrational absorption induced photothermal effect via a visible light source. A Mid-infrared photothermal probe (MI-PTP, or MIP) approach achieves 10 mM detection sensitivity and sub-micron lateral spatial resolution. Such performance exceeds the diffraction limit of infrared microscopy and allows label-free three-dimensional chemical imaging of live cells and organisms. Distributions of endogenous lipid and exogenous drug inside single cells can be visualized. MIP imaging technology may enable applications from monitoring metabolic activities to high-resolution mapping of drug molecules in living systems, which are beyond the reach of current infrared microscopy.

A digital microscope system comprises a plurality of camera systems for imaging a target region of an object, each camera system comprising a digital camera and an optical imaging system being aligned along an optical axis of said camera system, wherein the optical axes of the camera system are parallel to each other; a microscope stage, on which the object is to be arranged; a positioning device; and a controller configured to control the positioning device to move the plurality of camera systems and the microscope stage relative to each other orthogonally to the optical axes of the camera systems for selectively aligning any one of the camera systems with the target region of the object.

An arrangement for light sheet microscopy including illumination optics with an illumination objective for illuminating a sample, located in a medium on a sample carrier aligned with respect to a plane reference surface, with a light sheet. The arrangement also includes detection optics with a detection objective. The arrangement further includes a separating layer system with at least one layer separating the medium from the illumination and detection objectives. The separating layer system contacts the medium by a base surface aligned parallel to the reference surface. A correction lens system, with at least one correction lens serving to reduce those aberrations which occur as a result of the oblique passage of illumination light and/or of light to be detected through interfaces of the separating layer system, is arranged between illumination objective and separating layer system and/or between detection objective and separating layer system.