The present disclosure disclose a microscopic device and a focusing method of the microscopic device, the microscopic device includes an object stage configured to carry a transparent carrier and drive the transparent carrier to translate along at least one direction, the transparent carrier includes at least two object positions; a microscopic objective located on a side of the object stage; a rangefinder located on a same side of the object stage as a microscopic objective and at a same height relative to the object stage, and the rangefinder is configured to measure a distance between a surface of each of the at least two object positions and the microscopic objective; a focusing module configured to adjust a position of the microscopic objective along a first direction based on the distance measured by the rangefinder, wherein the first direction is perpendicular to a carrying surface of the object stage.

Disclosed herein, inter alia, are compositions and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections, using carrier substrates.

Apparatus for positioning a microscope slide and comprising co-acting first and second clamping jaws attached to a robot arm and adapted to receive and carry a microscope slide.

Apparatus for positioning a microscope slide and comprising co-acting first and second clamping jaws attached to a robot arm and adapted to receive and carry a microscope slide.

The present invention comprises an illuminator, a slide holder, three detachable objective lenses and a phone mount. It designs with magnetically connected modules set can arrange differently to perform three-distinct functions: a slide-viewing microscope, macro photography camera and slide projector that used with smartphones and tablets. The three detachable objective lenses can be used without a slide holder and illuminator to serve as high powered macro-lenses. Using the low-powered objective lens with the included torch of smartphone enables projection for the sample slide, giving the most dramatic viewing experience across all microscopes. Also, the module block integrate augmented reality (AR) function which offers animated specimen viewing and conducting virtual experiments to enhance the learning experiences. AR is enabled by scanning a dedicated AR slide through the app.

The present invention comprises an illuminator, a slide holder, three detachable objective lenses and a phone mount. It designs with magnetically connected modules set can arrange differently to perform three-distinct functions: a slide-viewing microscope, macro photography camera and slide projector that used with smartphones and tablets. The three detachable objective lenses can be used without a slide holder and illuminator to serve as high powered macro-lenses. Using the low-powered objective lens with the included torch of smartphone enables projection for the sample slide, giving the most dramatic viewing experience across all microscopes. Also, the module block integrate augmented reality (AR) function which offers animated specimen viewing and conducting virtual experiments to enhance the learning experiences. AR is enabled by scanning a dedicated AR slide through the app.

The disclosed spacers for microscope slides may include a spacer body including a material configured to provide an optical focal reference for a microscope and a central opening in the spacer body sized and shaped to receive a cytological sample. The spacer body may have a thickness of about 20 μm or less. Methods of analyzing cytological samples may include disposing a cytological sample on a microscope slide adjacent to a spacer positioned on the microscope slide, focusing an optical module of a microscope to a focal plane using at least a portion of the spacer as a focal reference, and viewing the cytological sample through the optical module of the microscope at the focal plane. Various other related methods, systems, and devices are also disclosed.

The disclosed spacers for microscope slides may include a spacer body including a material configured to provide an optical focal reference for a microscope and a central opening in the spacer body sized and shaped to receive a cytological sample. The spacer body may have a thickness of about 20 μm or less. Methods of analyzing cytological samples may include disposing a cytological sample on a microscope slide adjacent to a spacer positioned on the microscope slide, focusing an optical module of a microscope to a focal plane using at least a portion of the spacer as a focal reference, and viewing the cytological sample through the optical module of the microscope at the focal plane. Various other related methods, systems, and devices are also disclosed.

An automatically detecting system with microscope includes a platform and a gripper. The platform has a carrying area, a fixed block, a movable block, and an elastic member. The carrying area is formed at the platform, the fixed block and the movable block are configured at opposite sides of the carrying area, and the elastic member is connected to the movable block. The gripper pushes the movable block through an inclined plane of the movable block when moving a slide to the carrying area. When the gripper puts the slide on the carrying area, the movable block and the fixed block hold the slide with the elastic restoring force of the elastic member, and the gripper closes and moves on the upper surface of the slide to flatten the slide. Therefore, the slide can be set on the platform stably and flatly to obtain the focal length precisely and rapidly.

The disclosed technology brings histopathology into the operating theatre, to enable real-time intra-operative digital pathology. The disclosed technology utilizes confocal imaging devices image, in the operating theatre, “optical slices” of fresh tissue—without the need to physically slice and otherwise process the resected tissue as required by frozen section analysis (FSA). The disclosed technology, in certain embodiments, includes a simple, operating-table-side digital histology scanner, with the capability of rapidly scanning all outer margins of a tissue sample (e.g., resection lump, removed tissue mass). Using point-scanning microscopy technology, the disclosed technology, in certain embodiments, precisely scans a thin “optical section” of the resected tissue, and sends the digital image to a pathologist rather than the real tissue, thereby providing the pathologist with the opportunity to analyze the tissue intra-operatively. Thus, the disclosed technology provides digital images with similar information content as FSA, but faster and without destroying the tissue sample itself.