An optical wavelength conversion device includes an optical wavelength conversion member configured to convert the wavelength of incident light; a heat dissipation member which is more excellent in heat dissipation than the optical wavelength conversion member; and a joint portion which joins the optical wavelength conversion member and the heat dissipation member together. The optical wavelength conversion member includes a plate-shaped ceramic fluorescent body and a reflecting film disposed on a heat dissipation member-side surface of the ceramic fluorescent body. The joint portion has a thermal conductivity of 120 W/mK or more. The joint portion has a melting point of 240° C. or higher.

The disclosure relates to a method and an imaging device for multi-color imaging using frequency-modulated illumination. The method comprises a step of providing electromagnetic radiation with a plurality of different wavelengths, comprising a step of modulating each wavelength with a different modulation frequency, a step of illuminating a target with the modulated electromagnetic radiation, in particular for excitation of a target, a step of sensing electromagnetic radiation emitted from the target, in particular the luminescence, more in particular the fluorescence, and a step of processing the data obtained in the step of sensing.

A light guiding unit includes a light guiding member that light enters and an angle converter that the light from the light guiding member enters. The light guiding member has a side surface and a light exiting end surface which intersects the side surface and via which the light exits. The angle converter includes a light incident section on which the light from the light guiding member is incident, a light exiting section via which the light incident on the angle converter exits, and a reflection section that reflects the light incident via the light incident section toward the light exiting section. The refractive index of the interior of the angle converter is greater than the refractive index of air. The refractive index of the interior of the light guiding member is greater than the refractive index of the interior of the angle converter. The light exiting end surface and part of the side surface are in contact with the angle converter.

A method of calibrating a fluorescence in-situ hybridization (FISH) system includes contacting a calibration slide with a plurality of probes, obtaining one or more images of the calibration slide; and calibrating the FISH system based on the one or more images. The calibration slide has a surface with a first plurality of beads. Each bead of the first plurality of beads has one or more binding domains. Each probe has a tag and a targeting domain, and the targeting domain binds a binding domain from the at least one binding domain.

A calibration slide for calibrating a multiplexed fluorescence in-situ hybridization (FISH) system has a substrate having a surface and a first plurality of beads on the surface. Each bead of the first plurality of beads has at least one binding domain to be bound to a corresponding probe that has a tag and a targeting domain that binds to a binding domain.

A scanning microscope includes a light source for generating a light beam having a wavelength, λ, and beam-forming optics configured for receiving the light beam and generating a quasi-Bessel excitation beam that is directed into a sample. The quasi-Bessel beam has a lateral FWHM and an axial FWHM that is greater than ten times the lateral FWHM, and the beam-forming optics include an excitation objective having an axis oriented in a first direction. The microscope includes beam scanning optics configured for scanning the quasi-Bessel beam in one or more directions that are substantially perpendicular to the first direction, and a detector configured for detecting signal light received from positions within the sample that are illuminated by the quasi-Bessel beam. The signal light is generated in response to an interaction of the excitation beam with the sample, and the signal light is imaged, at least in part, by the excitation objective, onto the detector.

A wavelength conversion member includes: a substrate; a wavelength converter including phosphor particles excited by excitation light and a binder layer that fixes or adheres the adjacent phosphor particles to one another, the wavelength converter being provided on a front surface side of the substrate; and a light reflecting film that reflects fluorescent light radiated by the phosphor particles, the light reflecting film being provided on at least a part of an interface between the substrate and the wavelength converter, wherein a refractive index of the phosphor particles is larger than a refractive index of the binder layer. It is preferable that the binder layer include nanogaps which are voids with an average diameter of 300 nm or less in an inside.

A microscope includes illumination optics for fluorescence excitation of point light sources of a sample, detection optics and a camera having a sensor. A density of the point light sources is kept low so as to minimize a crossover of point light sources that are behind or close to one another in each image captured by the camera. A means for subdividing a detection aperture into individual sub-apertures is provided in a beam path of the detection optics such that images generated by the individual sub-apertures on the sensor of the camera depict an object volume from different spatial directions.

Disclosed is a fluorescent fundus camera which is one kind of ophthalmological diagnostic equipment, using a blue excitation light beam when a contrast medium appears in the retinal vessels. When an abnormal blood vessel is present, the contrast medium leaks into the retina or appears as color in tissues surrounding the retina, and the presence of damage to the retina and the appearance of abnormal neovascularization can be found.

The present disclosure discloses a color filter substrate, a manufacture method thereof and a display device. The color filter substrate includes a substrate; and a light channel layer on a side of the substrate. The light channel layer includes a first photonic crystal layer and a second photonic crystal layer stacked with each other up and down. The light channel layer includes a plurality of light channel units formed by a periodic arrangement of three different primary-color light channel units. Each of the light channel units includes a photonic crystal block of the first photonic crystal layer and a photonic crystal block of the second photonic crystal layer with an orthographic projection of the photonic crystal block of the first photonic crystal layer on the substrate overlapping an orthographic projection of the photonic crystal block of the second photonic crystal layer on the substrate.