A compound microscope system, according to one embodiment, may comprise: a sample holder for supporting a sample; an optical interference microscope module for transferring a broadband light towards the sample and a reference mirror, and forming a tomographic image of the sample on the basis of a spectrum image formed by the mutual interference between lights reflected from the sample and the reference mirror; and a nonlinear microscope module for forming a nonlinear image of the sample by irradiating a laser pulse towards the sample, and then measuring an excitation light comprising excited multiphoton.

Various embodiments of a multi-photon microscopy system that uses sequential excitation of a sample through three or more objective lenses oriented at different axes intersecting the sample are disclosed. Each objective lens is capable of focused sequential excitation of the sample that elicits fluorescence emissions from the excited sample, which is then simultaneously detected by each respective objective lens along a respective longitudinal axis every time the sample is illuminated through only a single objective lens.

Aspects of the subject disclosure may include, for example, identifying a request to facilitate communications between first and second processing nodes, determining that the communications are to be established via quantum teleportation between, and identifying a network path comprising a first path segment to obtain a quantum channel, wherein quantum entanglement is established between the first and second processing nodes based on transportation of a first quantum entangled object via the quantum channel. A classical communication channel is facilitated between the first and second processing nodes, adapted to exchange between the nodes, quantum state information of a measurement performed upon the first quantum entangled object. Information is exchanged between the first and second processing nodes via the quantum channel according to the transported first quantum entangled object and the exchanged quantum state information. Other embodiments are disclosed.

Disclosed herein are systems and methods for line excitation array detection (LEAD) microscopy. The systems and methods include an excitation beam from an optical beam source and a subject of interest. Light is scanned across the subject of interest and optical signals are detected using a parallel optical detection means. A number of mechanical, acoustic and or optical components such as scanning mirrors, DMDs, OADs, electric motors may be used in separately or in conjunction to aid in the scanning of the excitation beam across the subject of interest

A method and an apparatus for imaging a sample (14). In the method, a first excitation radiation (5) is focused into a volume of the sample (14) and a caused first detection radiation (15) is captured and evaluated in respect of a form of its wavefront. A second excitation radiation (11) is manipulated on the basis of the evaluation results in order to correct the ascertained deviations of the wavefront. A region (20) to be imaged of the sample (14) is scanned by means of the second excitation radiation (11) and a second detection radiation (16) is captured as image data. The second excitation radiation (11) is directed in the form of at least two partial beams (11T) into the sample volume, into a respective spot (22) illuminated by the partial beam (11T) and the second detection radiations (16) respectively caused by the partial beams (11T) are captured separately.

A two-photon stimulated emission depletion composite microscope, comprising a two-photon imaging unit (100) and an STED imaging unit (200), wherein the two-photon imaging unit (100) can be used for a relatively thick sample, and the STED super-resolution imaging unit can be used for a region of interest on a surface of a sample, and the microscope makes light spots generated by an excitation light and a depletion light after being focused by an objective lens (OL) accurately coincide in a three-dimensional distribution. The two-photon stimulated emission depletion composite microscope (10) integrates two functions of STED imaging and two-photon imaging and makes the two types of light spots generated by an excitation light and a depletion light after being focused by an objective lens accurately coincide in a three-dimensional distribution, thereby providing a powerful tool for cutting-edge biomedical research.

Fluorescent compounds that have aggregation-induced emission (AIE) characteristics. The compounds can be utilized as lipid droplet (LD)-specific bio-probes in cell imaging, with high photostability and brightness. For example, the compounds can be used for specific two-photon LDs staining in live cells and deep-tissues at ultralow concentrations. The compounds exhibit a large Stokes shift (>110 nm), high solid fluorescence quantum yields (up to 0.30), a good two-photon absorption cross-section (45-100 GM at 860 nm), high biocompatibility, and good photostability.

An image acquisition method in which a pulsed illumination beam emitted from a light source is scanned while being focused at a sample, signal light generated as a result of a non-linear optical process at each scanning position is detected, and an image of the sample is generated on a basis of the detected signal light, the image acquisition method including: acquiring a mixed image, which includes in-focus signal light generated at a focal position of the illumination beam in the sample and which also includes out-of-focus signal light; acquiring an image of the out-of-focus signal light on a basis of a plurality of mixed images having mutually different intensities of the out-of-focus signal light; and acquiring an image of the in-focus signal light by subtracting the image of the out-of-focus signal light acquired, from the mixed image acquired.

An exemplary device can be provided which can include, for example, a radiation source(s) configured to generate a first radiation(s), a spatial light modulator (SLM) arrangement(s) configured to receive the first radiation(s) and generate a second radiation(s) based on the first radiation(s), and a galvanometer(s) configured to receive the second radiation(s), generate a third radiation(s) based on the second radiation(s), and provide the third radiation(s) to a sample(s).

A light sheet imaging system, such as a light sheet microscope, comprises an illumination arrangement for generating a light sheet for three-photon excitation of a fluorescent sample, and a fluorescence collection arrangement for collecting fluorescence generated in the sample as a result of three-photon excitation by the light sheet. The light sheet may be a non-diffractive, propagation-invariant light sheet. The light sheet may be formed from and/or comprise a Bessel beam. A method of light sheet imaging comprises using a light sheet for three-photon excitation of a fluorescent sample, and collecting fluorescence generated in the sample as a result of three-photon excitation of the sample by the light sheet. Such a method may be used for light sheet microscopy.