A system for generating a series of reducing intensity laser pulses from an ultrafast pulsed laser source. The reducing intensity series of pulses is equally temporally spaced between pulses from the laser source. The repeating series of reducing intensity laser pulses is fed to a microscope for imaging. The microscope is capable of detecting the fluorescence from a sample generated by each pulse in the series. The data is processed using knowledge of the pulse intensity, location on the test sample, and amount of fluorescence measured to create an increased dynamic range of the image relative to what can be obtained in a normal two-photon imaging system.

Various embodiments of a multi-photon microscopy system that uses sequential excitation of a sample through three or more objective lenses oriented at different axes intersecting the sample are disclosed. Each objective lens is capable of focused sequential excitation of the sample that elicits fluorescence emissions from the excited sample, which is then simultaneously detected by each respective objective lens along a respective longitudinal axis every time the sample is illuminated through only a single objective lens.

A method and system for improving lateral resolution in optical microscopy are provided. The method includes generating a source optical beam and passing the source optical beam successively through an axicon, a Fourier-transform lens and an objective to convert the source optical beam into an excitation Bessel-type beam having a central lobe and at least one side lobe. The method also includes focusing the excitation beam onto a focal plane of the objective within or on a sample to generate a sample light signal, and spatially filtering the sample light signal. The spatial filtering includes rejecting light originating from outside of the focal plane and light generated by the at least one side lobe of the excitation beam. The spatial filtering also includes permitting passage, as a filtered light signal, of light generated by the central lobe of the excitation beam. The method further includes detecting the filtered light signal.

A high-power mode-locked laser system is disclosed herein which includes at least one pump source, at least one laser cavity formed by at least one high reflector and at least one output coupler, and at least one ytterbium-doped optical crystal positioned within the laser cavity in communication with the pump source, the ytterbium-doped optical crystal configured to output at least one output signal of at least 20 W, having a pulse width of 200 fs or less, and a repetition rate of at least 40 MHz.

A multiphoton microscope based on two-photon excited fluorescence and second-harmonic generation that images FOVs of about 0.8 mm.sup.2 (without stitching adjacent FOVs) at speeds of 10 frames/second (800800 pixels) with lateral and axial resolutions of 0.5 m and 2.5 m, respectively. The scan head of the instrument includes a fast galvanometric scanner, relay optics, a beam expander and a high NA objective lens. The system is based on a 25, 1.05 NA water immersion lens, which features a long working distance of 1 mm. A proper tailoring of the beam expander, which consists of the scan and tube lens elements, enables scaling of the FOV. The system and method also include a flat wavefront of the beam, minimum field curvature, and suppressed spherical aberrations. All aberrations in focus are below the Marechal criterion of 0.07 rms for diffraction-limited performance.

A system and method for adaptive illumination, the imaging system comprising an excitation source having a modulator, which generates a pulse intensity pattern having a first wavelength when the excitation source receives a modulation pattern. The modulation pattern is a data sequence of a structural image of a sample. An amplifier of the imaging system is configured to receive and amplify the pulse intensity pattern from the modulator. A frequency shift mechanism of the imaging system shifts the first wavelength of the pulse intensity pattern to a second wavelength. A laser scanning microscope of the imaging system receives the pulse intensity pattern having the second wavelength.

Provided are a miniature endoscopic probe and a multi-photon endoscopy including the same.

A multi-photon wavefront sensor system and method. The system includes a Shack-Hartmann wavefront sensor and a laser excitation source configured to emit a plurality of laser pulses at a wavelength in the near-infrared range, wherein the plurality of laser pulses are configured to induce multi-photon absorption in a detector material of the Shack-Hartmann wavefront sensor.

A high-power ytterbium-doped calcium fluoride laser system is disclosed herein which includes at least one pump source, at least one laser cavity formed by at least one high reflector and at least one output coupler, and at least one ytterbium-doped calcium fluoride optical crystal positioned within the laser cavity in communication with the pump source, the ytterbium-doped calcium fluoride optical crystal configured to output at least one output signal of at least 20 W, having a pulse width of 200 fs or less, and a repetition rate of at least 40 MHz.

An exemplary device can be provided which can include, for example, a radiation source(s) configured to generate a first radiation(s), a spatial light modulator (SLM) arrangement(s) configured to receive the first radiation(s) and generate a second radiation(s) based on the first radiation(s), and a galvanometer(s) configured to receive the second radiation(s), generate a third radiation(s) based on the second radiation(s), and provide the third radiation(s) to a sample(s).