Example embodiments relate to imaging devices for in-line holographic imaging of objects. One embodiment includes an imaging device for in-line holographic imaging of an object. The imaging device includes a set of light sources configured to output light in confined illumination cones. The imaging device also includes an image sensor that includes a set of light-detecting elements. The set of light sources are configured to output light such that the confined illumination cones are arranged side-by-side and illuminate a specific part of the object. The image sensor is arranged such that the light-detecting elements detect a plurality of interference patterns. Each interference pattern is formed by diffracted light from the object originating from a single light source and undiffracted light from the same single light source. At least a subset of the set of light-detecting elements is arranged to detect light relating to not more than one interference pattern.

Example methods, apparatuses, and computer program products related to analyzing fluid samples are provided. For example, an example computer-implemented method for analyzing fluid samples includes receiving digital holography image data associated with a fluid sample in a flow chamber device; extracting, from the digital holography image data, an upper reference mark image region associated with an upper reference mark and a lower reference mark image region associated with a lower reference mark; determining a maximum focal depth and a minimum focal depth associated with the digital holography image data, respectively; focusing each of a plurality of focal depth layers associated with the digital holography image data; and extracting, from the plurality of focal depth layers, one or more region of interest (ROI) portions that are associated with the fluid sample.

A method of characterizing one or more particles in a fluid, e.g. a liquid, using interferometric scattering optical (iSCAT) microscopy. The method involves illuminating a region of a fluid using an objective lens so that light is scattered by one or more particles in the fluid. The scattered light and reference light are captured using the objective lens and interfere at an imaging device. A succession of images of the interference is processed to determine image correlation values which define a gradual decorrelation over time from which a property of the particle(s) is determined.

An image processing apparatus includes an acquisition unit that acquires a hologram obtained by imaging a plurality of granules contained within an imaging visual field, a generation unit that generates, from the hologram, phase difference images at positions different from each other in an optical axis direction in a case in which the hologram is captured, a specifying unit that specifies a plurality of image ranges in a direction of a plane intersecting the optical axis direction, which correspond to the plurality of granules, in an averaged image obtained by averaging at least some of the phase difference images, and an extraction unit that extracts the phase difference image at a center position of a corresponding granule in the optical axis direction for each of the plurality of image ranges.

An image processing apparatus includes an acquisition unit that acquires a hologram obtained by imaging a plurality of granules contained within an imaging visual field, a generation unit that generates, from the hologram, phase difference images at positions different from each other in an optical axis direction in a case in which the hologram is captured, a specifying unit that specifies a plurality of image ranges in a direction of a plane intersecting the optical axis direction, which correspond to the plurality of granules, in an averaged image obtained by averaging at least some of the phase difference images, and an extraction unit that extracts the phase difference image at a center position of a corresponding granule in the optical axis direction for each of the plurality of image ranges.

A machine is configured to perform hologram location within a scene to be generated. The machine accesses target motion data that describes movement of a target device. Based on the target motion data, the machine determines a target motion vector that indicates a target speed of the target device and indicates a target direction in which the target device is moving. The machine determines a hologram motion vector for a hologram to be generated for display by a user device. The hologram motion vector indicates a relative speed of the hologram and indicates a relative direction of movement for the hologram. The machine then causes the user device to generate a scene in which the hologram moves at a speed determined based on the target speed and on the relative speed, as well as moves in a direction determined based on the target direction and on the relative direction.

An in-line holographic microscope can be used to analyze a video stream to track individual colloidal particles' three-dimensional motions. The system and method can provide real time nanometer resolution, and simultaneously measure particle sizes and refractive indexes. An assay using the holographic microscope for holographic particle characterization directly detect viruses, antibodies and related targets binding to the surfaces of specifically functionalized micrometer-scale colloidal probe beads. The system detects binding of targets by directly measuring associated changes in the bead's diameter without the need for downstream labeling and analysis.

A system for generating holographic images or videos comprising a camera array, a plurality of processors, and a central computing system. A method for generating holographic images can include receiving a set of images and processing the images.

Systems, methods, and computer-readable media are disclosed for representing a transfer of a digital object using holographic images. User input is received that is indicative of a selection of the digital object for transfer from a sending device to a receiving device. Spatial attribute data is generated based at least in part on at least one of a distance or a relative orientation between the sending device and the receiving device, and a transition path is determined based at least in part on the spatial attribute data. Holographic image data is then generated based at least in part on the transition path, and the holographic image data is sent to one or more holographic projectors to cause a first holographic image representative of the digital object and a second holographic image representative of the transition path to be projected.

A method and system for improving holographic image simulation and presentation is provided. The method includes receiving and analyzing audio and video data associated with historical tendencies of an opponent sporting team occurring during previous sporting contests involving the opponent sporting team. Predicted tendencies of the opponent sporting team are determined with respect to a future sporting contest scheduled with a first sporting team. In response, a holographic simulation presentation is generated. The holographic simulation presentation is associated with a predicted performance of players of the opponent sporting team with respect to the future sporting contest scheduled with the first sporting team. The holographic simulation presentation is presented such that players of the first sporting team interact with holographic images of the players of the opponent sporting team during a practice session.