A data generating device sets an initial candidate solution of an intensity spectrum function, a phase spectrum function, and an initial temperature and a cooling rate, generates a neighborhood solution, transforms a first waveform function of a frequency domain including the neighborhood solution and the phase spectrum function into a second waveform function of a time domain including a time-intensity waveform function and a time-phase waveform function and calculates an evaluation value representing a degree of difference between the time-intensity waveform function and the desired time-intensity waveform, sets the neighborhood solution as an n-th candidate solution for a certain probability, and lowers the temperature on the basis of the cooling rate. A decrease in the temperature acts in a direction in which the probability P is lowered when the evaluation value of the neighborhood solution is worse than the evaluation value of the candidate solution.

Laser 3D imaging techniques include splitting a laser temporally-modulated waveform of bandwidth B and duration D from a laser source into a reference beam and a target beam and directing the target beam onto a target. First data is collected, which indicates amplitude and phase of light relative to the reference beam received at each of a plurality of different times during a duration D at each optical detector of an array of one or more optical detectors perpendicular to the target beam. Steps are repeated for multiple sampling conditions, and the first data for the multiple sampling conditions are synthesized to form one or more synthesized sets. A 3D Fourier transform of each synthesized set forms a digital model of the target for each synthesized set with a down-range resolution based on the bandwidth B.

Apparatus and methods for 3D-Scanless Holographic Optogenetics with Temporal focusing (3D-SHOT), which allows precise, simultaneous photo-activation of arbitrary sets of neurons anywhere within the addressable volume of the microscope. Soma-targeted (ST) optogenetic tools, ST-ChroME and IRES-ST-eGtACR 1, optimized for multiphoton activation and suppression are also provided. The methods use point-cloud holography to place multiple copies of a temporally focused disc matching the dimensions of a designated neuron's cell body. Experiments in cultured cells, brain slices, and in living mice demonstrate single-neuron spatial resolution even when optically targeting randomly distributed groups of neurons in 3D.

The present disclosure relates to systems and methods for cell recognition. At least one embodiment relates to a method for recognizing cell. The method includes receiving an image of the cell. The method also includes performing edge detection on the image of the cell. Further, the method includes detecting ridges within the image of the cell. In addition, the method includes quantifying an internal complexity of the cell by gauging a contrast of the ridges with an average of a Laplacian on the detected ridges.

A laser fabrication method and a laser fabrication system. The laser fabrication system includes an ultrafast laser source configured to output a laser beam; and a digital micromirror device (DMD), configured to receive, shape, and scan the laser beam, wherein more than one binary holograms are synthesized to form a scanning hologram applied to the DMD. The shaped laser beam, containing one or multiple focal points, leaving the DMD, are focused to the sample for fast laser fabrication.

A holographic display includes a pixel array including one or more pixels, a holographic data source, a drive array, and a light source. Each pixel includes a phase-modulating structure and an amplitude-modulating structure. The holographic data source is configured to supply a holographic drive signal. The holographic drive signal includes, for each pixel in each of a plurality of time-varying holographic image frames, a phase component defining phase modulation for the pixel and an amplitude component defining amplitude modulation for the pixel. The drive array is configured to, for each pixel in each holographic image frame, modulate the phase-modulating structure according to the phase component and modulate the amplitude-modulating structure according to the amplitude component. The light source is configured to output coherent light to illuminate the one or more pixels of the pixel array.

The invention refers to an Illumination device for illuminating a spatial light modulator device. Sub-holograms are used for encoding a hologram into the spatial light modulator device. The Illumination device comprises at least one light source for emitting light for illuminating the spatial light modulator device and a beam shaping unit. The beam shaping unit provides a flat-top plateau-type distribution of an absolute value of a complex degree of mutual coherence of the light in a plane of the spatial light modulator device to be illuminated. The flat-top plateau-type distribution of the absolute value of the complex degree of mutual coherence has a shape that is at least similar to a shape of the largest sub-hologram used for encoding of object points into the spatial light modulator device.

An example system includes a master oscillator that provides a master oscillator beam, a first fiber beam splitter that splits the master oscillator beam into a first branch and a second branch, a second fiber beam splitter that splits the first branch into an imaging branch and an amplified characteristic branch, and a compensation branch mixer that mixes the second branch and the amplified characteristic branch into a compensation branch. The example system includes transmission optics that direct the imaging branch to a target location, an imaging mixer that mixes the compensation branch and a reflected image from the target location into an imaging signal, and an acquisition device that receives the imaging signal as a final image. An example system optionally includes a mode matching lens that applies a selected wavefront characteristic to the compensation branch.

A plasma generator including: a femtosecond light source that generates a laser pulse beam; a processor that computes a computer generated hologram; a spatial light modulator that modifies the laser pulse beam in accordance with the computer generated hologram; a three dimensional scanner optically coupled to the spatial light modulator to direct the modified laser pulse beam to one or more focal points in air; and a lens that focuses the modified laser pulse beam. The modified laser pulse beam induces a light emission effect at a one or more focal points that can be visible, audible, and palpable.

An optical data-recording system comprises a laser, a dynamic digital hologram, an electronic controller, and a scanning mechanism. The dynamic digital hologram includes a plurality of holographic zones, and is configured to direct the irradiance received thereon to an optical recording medium. The electronic controller is operatively coupled to the dynamic digital hologram and configured to control the irradiance directed from each of the holographic zones. The scanning mechanism is configured to change a relative positioning of the laser versus the dynamic digital hologram so that each of the holographic zones is irradiated in sequence by the laser.