Digital holographic microscopy and related image processing techniques are described. A hologram captured in an image frame is split into different depths while a new hologram is being captured. Image slices of the hologram are determined and using free space impulse responses that are pre-calculated at a different precision than processing operations using the holographic data. Each computation is calculated in parallel based on the number of available processing cores and threads. The image slices are combined into a 2D array or 3D array to permit further processing of the combined array to count and size particles in the image frame. The reconstructed hologram is displayed at a subsequent image frame than that used to capture the hologram.

A device in holographic imaging comprises: at least two light sources, wherein each of the at least two light sources is arranged to output light of a unique wavelength; and at least one holographic optical element, wherein the at least two light sources and the at least one holographic optical element are arranged in relation to each other such that light from the at least two light sources incident on the at least one holographic optical element interacts with the at least one holographic optical element to form wavefronts of similar shape for light from the different light sources.

A hologram that includes a formation layer and a reflection layer that are laminated. The formation layer has an optical phase modulation structure on a first interface in contact with the reflection layer. When reference light emitted from a point light source enters through a second interface different from the first interface of the formation layer, the entirety or part of an image to be reconstructed by the optical phase modulation structure is reconstructed as spatial information on the point light source side relative to the second interface.

A method is disclosed for classifying and/or counting objects (for example, cells) in an image that contains a mixture of several types of objects. Prior statistical information about the object mixtures (class proportion data) is used to improve classification results. The present technique may use a generative model for images containing mixtures of object types to derive a method for classifying and/or counting cells utilizing both class proportion data and classified object templates. The generative model describes an image as the sum of many images with a single cell, where the class of each cell is selected from some statistical distribution. Embodiments of the present techniques have been successfully used to classify white blood cells in images of lysed blood from both normal and abnormal blood donors.

Provided are correction pattern obtaining apparatuses and methods of obtaining a correction pattern by using the correction pattern obtaining apparatuses. The correction pattern obtaining apparatus includes a flat panel display having an optical element that receives one or more input test patterns, a detector that measures intensity of light emitted from the flat panel display corresponding to each of the one or more input test patterns and a processor that determines a correction pattern comprising one or more of the one or more test patterns at a given ratio based on the measured intensity of light corresponding to each of the one or more input test patterns.

Example embodiments relate to imaging devices for in-line holographic imaging of objects. One embodiment includes an imaging device for in-line holographic imaging of an object. The imaging device includes a set of light sources configured to output light in confined illumination cones. The imaging device also includes an image sensor that includes a set of light-detecting elements. The set of light sources are configured to output light such that the confined illumination cones are arranged side-by-side and illuminate a specific part of the object. The image sensor is arranged such that the light-detecting elements detect a plurality of interference patterns. Each interference pattern is formed by diffracted light from the object originating from a single light source and undiffracted light from the same single light source. At least a subset of the set of light-detecting elements is arranged to detect light relating to not more than one interference pattern.

Method and system for lensless, shadow optical imaging. Formation of a hologram shadow image having higher spatial resolution and lower noise level is accomplished by processing image information contained in multiple individual hologram shadow image frames acquired either under conditions of relative shift between point light source and the detector of the system or under stationary conditions, when system remains fixed in space and is devoid of any relative movement during the process of acquisition of individual image frames.

Systems and methods for controlling optical amplitude and phase of incident electromagnetic are provided, wherein an exemplary system comprises a substrate and a plurality of meta units, attached to the top surface of the substrate and configured to convert the incident electromagnetic radiation into a target electromagnetic radiation by modifying both optical amplitude and phase.

An ultra high-resolution near infrared brain imager system includes a modular cap housing closely spaced multiple vertical-cavity surface-emitting lasersingle-photon avalanche photodiode array (VCSEL-SPAD) modules, each one of the VCSEL-SPAD modules including a linear VCSEL array and a SPAD detector.

The present disclosure relates to devices and methods configured to perform drug screening on cells. At least one embodiment relates to a lens-free device for performing drug screening on cells. The lens-free device includes a substrate having a surface. The lens-free device also includes a light source positioned to illuminate the cells, when present, on the substrate surface with a light wave. The lens-free device further includes a sensor positioned to detect an optical signal caused by illuminating the cells. The substrate surface includes a microelectrode array for sensing an electrophysiological signal from the cells.