A method of calibration of a device for analyzing at least one element present in a sample, said device including: a detection assembly configured to acquire an image formed by the interference between a light source and said sample; and digital processing means configured to detect a digital position of at least one element in said sample based on said acquired image; said calibration method including the implementation of a plurality of predetermined displacements of said sample with respect to said detection assembly and, for all of said displacements, the detection of a digital position of a same element to determine the digital position and the real position matching model according to the predetermined displacements and to the digital positions of said element after each displacement.

A display device includes a light source part including a first light source and a second light source, a collimation lens that collimates light incident from the light source part, a wave guide that guides and diffracts the light incident from the collimation lens, a spatial light modulator that modulates the light passing through the wave guide so as to form a holographic pattern for reproducing a holographic image, a focusing optical system that focuses the holographic image on a space, and a light separating plate disposed between the light source part and the collimation lens, the light separating plate separating light of the first light source and light of the second light source from each other.

Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.

A display apparatus includes a coherent light source, a display unit, a light-diffusing element, and at least one optical element. The coherent light source is configured to provide coherent light beams. The display unit is configured to form a three-dimensional image beam based on interference of the coherent light beams, wherein the three-dimensional image beam is imaged on an intermediate imaging surface after passing through the display unit. The light-diffusing element is located on the intermediate imaging surface, wherein a diffusion angle of the three-dimensional image beam is sequentially changed by passing through the light-diffusing element. The at least one optical element is located on a transmission path of the three-dimensional image beam from the light-diffusing element, and is configured to project the three-dimensional image light beam passing through the display unit out of the display apparatus to display a three-dimensional image.

A predetermined lighting pattern is projected on a surface to be illuminated and the lighting pattern is displaced on the surface to be illuminated. A laser beam generated by a laser light source is broadened by a magnifying lens so as to generate a divergent light. The divergent light is shaped by the collimation lens into a parallel illumination light, and the parallel illumination light is caused to be incident on an incident plane of a diffraction optical element which records a hologram image. A diffracted light from the diffraction optical element forms the lighting pattern as a hologram reconstructed image on the surface to be illuminated. By translating the collimation lens by a collimation-lens drive unit along a movement plane that is orthogonal to an optical axis of the laser beam, the lighting pattern can be displaced on the surface to be illuminated.

In a microscopic observation unit (10), hologram data is acquired at each measurement position on a cell culture plate (13) while a light-source section (11) and other related sections are gradually moved by a moving section (15). Every time a set of data for one measurement position is acquired, a measurement monitoring image creator (24) creates a thumbnail image by reducing the size of a hologram image which is based on original data (two-dimensional distribution of light intensity). A display processor (25) pastes the create thumbnail image to progressively complete the hologram image of the entire plate to be displayed on a display unit (27). A measurement operator watches the hologram image during the execution of the measurement. When it has been concluded that the ongoing measurement is inappropriate, the operator presses a measurement stop button to immediately discontinue the measurement. Thus, When there is a problem with the measurement, such as a foreign object mixed in the sample, the measurement can be discontinued before a phase image or intensity image based on the hologram data is reconstructed on the server after the completion of the entire measurement, so as to avoid wasting time for the useless measurement.

Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.

Disclosed herein is a consumer laser light device for producing laser light effects with the use of an optical effects wheel. In some respects, the disclosure is direct to a device for selectively providing one of multiple optical effects manipulating a laser beam, including an optical effects wheel positioned in the light path, the optical effects wheel having a first optical effect engraved on a first portion of the optical effects wheel and a second optical effect engraved on a second portion of the optical effects wheel, wherein the first portion and the second portion partially overlap. The optical effects wheel may be further modified to ensure that the device complies with consumer safety requirements for laser light devices.

A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.

A holographic sight comprises a unitary optical component carrier. The unitary optical component carrier may comprise a body with a first receptacle configured to receive a laser diode, a second receptacle configured to receive a mirror, a third receptacle configured to receive a collimating optic, a fourth receptacle configured to receive a grating, and a fifth receptacle configured to receive an image hologram. A laser diode may be received within opposing walls formed by the first receptacle. A mirror may be received in, and abut one or more surfaces of the second receptacle. A collimating optic may be received in, and abut one or more surfaces of the third receptacle. A grating may be received in, and abut one or more surfaces of the fourth receptacle. A hologram image may be received in, and abut one or more surfaces of the fifth receptacle.