A nucleic acid storage system (100) that uses nanopore sequencing to read data values chemically embedded in oligonucleotides includes a membrane (102), a voltage source (108), and a nucleic acid strand (110). The membrane (102) has a plurality of nanopores (104) that are stacked upon one another in a multi-nanopore arrangement. The voltage source (108) is configured to direct voltage across the plurality of nanopores (104). The nucleic acid strand (110) including the oligonucleotides is threaded through each of the plurality of nanopores (104) within the membrane (102). A separate base signal (118) is generated from the nucleic acid strand (110) being threaded through each of the plurality of nanopores (104), and Recursive Neural Networks can be used to estimate a signal shape for each oligonucleotide. Recurrent Convolutional Neural Networks and noise predictive data detection algorithms can be used based on the estimated signal shapes to sequence the oligonucleotides.

A nucleic acid storage system (100) that uses nanopore sequencing to read data values chemically embedded in oligonucleotides includes a membrane (102), a voltage source (108), and a nucleic acid strand (110). The membrane (102) has a plurality of nanopores (104) that are stacked upon one another in a multi-nanopore arrangement. The voltage source (108) is configured to direct voltage across the plurality of nanopores (104). The nucleic acid strand (110) including the oligonucleotides is threaded through each of the plurality of nanopores (104) within the membrane (102). A separate base signal (118) is generated from the nucleic acid strand (110) being threaded through each of the plurality of nanopores (104), and Recursive Neural Networks can be used to estimate a signal shape for each oligonucleotide. Recurrent Convolutional Neural Networks and noise predictive data detection algorithms can be used based on the estimated signal shapes to sequence the oligonucleotides.

The present invention relates to a method for determining one or more intrinsic properties of a DNA component from a plurality of measurements obtained over a time period from a cell culture, with each cell comprising the DNA component, wherein the DNA component is involved in transcription of one or more target signals, wherein the plurality of measurements comprises measurements relating to the density of the cell culture over the time period and measurements relating to the amount of the one or more target signals in the cell culture over the time period.

The present systems and methods introduce deep learning to de novo peptide sequencing from tandem mass spectrometry data, and in particular mass spectrometry data obtained by data-independent acquisition. The systems and methods achieve improvements in sequencing accuracy over existing systems and methods and enables complete assembly of novel protein sequences without assisting databases. To sequence peptides from mass spectrometry data obtained by data-independent acquisition, precursor profiles representing intensities of one or more precursor ion signals associated with a precursor retention time and fragment ion spectra representing signals from fragment ions and fragment retention times are fed into a neural network.

The present systems and methods introduce deep learning to de novo peptide sequencing from tandem mass spectrometry data, and in particular mass spectrometry data obtained by data-independent acquisition. The systems and methods achieve improvements in sequencing accuracy over existing systems and methods and enables complete assembly of novel protein sequences without assisting databases. To sequence peptides from mass spectrometry data obtained by data-independent acquisition, precursor profiles representing intensities of one or more precursor ion signals associated with a precursor retention time and fragment ion spectra representing signals from fragment ions and fragment retention times are fed into a neural network.

The technology disclosed relates to equalizer-based intensity correction for base calling. In particular, the technology disclosed relates to accessing an image whose pixels depict intensity emissions from a target cluster and intensity emissions from additional adjacent clusters, selecting a lookup table that contains pixel coefficients that are configured to increase a signal-to-noise ratio, applying the pixel coefficients to intensity values of the pixels in the image to produce an output, and base calling the target cluster based on the output.

The technology disclosed relates to equalizer-based intensity correction for base calling. In particular, the technology disclosed relates to accessing an image whose pixels depict intensity emissions from a target cluster and intensity emissions from additional adjacent clusters, selecting a lookup table that contains pixel coefficients that are configured to increase a signal-to-noise ratio, applying the pixel coefficients to intensity values of the pixels in the image to produce an output, and base calling the target cluster based on the output.

Techniques for improving artificial intelligence-based base calling are disclosed. The improved techniques can be used to better train artificial intelligence for base calling by reordering of sequencing images, and training of a neural network-based base caller where the temporal logic is effectively “frozen” (or bypassed). In addition, the improved techniques include various combinations, including, for example, combining “normalization” of sequencing images with reordering of sequencing images and/or with effectively “freezing” the temporal logic.

A random emulsification digital absolute quantitative analysis method includes: performing random emulsification processing on a system to be emulsified to obtain several isolated reaction zones or droplets; determining the total number and volume information of the various reaction zones or droplets, the presence of target molecules to be tested in the respective reaction zones or droplets, and the number of reaction zones or droplets which do not contain the target molecules by combining acquired target images comprising image regions corresponding to the amplified reaction zones or droplets, and analyzing the target images; and accurately calculating the volume information of the various reaction zones or droplets, the presence of the target molecules to be tested in the respective reaction zones or droplets, and the number of reaction zones or droplets which do not contain the target molecules, the total number of target molecules in a sample to be tested.

This disclosure describes methods, non-transitory computer readable media, and systems that can generate signal-to-noise-ratio metrics for clusters of oligonucleotides to which tagged nucleotide bases are added and utilize the signal-to-noise-ratio metrics to generate nucleotide-base calls and determine base-call quality. For example, the disclosed systems can generate the signal-to-noise-ratio metrics using scaling factors and noise levels associated with light signals detected from the clusters of oligonucleotides. The disclosed systems can utilize the signal-to-noise-ratio metrics to generate intensity-value boundaries for generating nucleotide-base-calls for the signals in accordance with one or more base-call-distribution models. Additionally, the disclosed systems can utilize a threshold to filter out signals detected from the clusters of oligonucleotides that have low signal-to-noise-ratio metrics. The disclosed systems can further utilize the signal-to-noise-ratio metrics to generate quality metrics for generated nucleotide-base calls.