A device may determine a calibration value for a spectrometer using light from a first light source; deactivate the first light source after determining the calibration value; perform measurement with regard to a sample based on the calibration value, wherein the measurement of the sample is performed using light from a second light source; determine that the calibration value is to be updated; and update the calibration value using the light from the first light source.

Provided is a microscope system including a microscope provided with a multi-channel image-acquisition unit that acquires images of a specimen at respective wavelengths; an adjustment-method storage portion that stores, for respective channels, contrast adjusting methods for the images acquired by the image-acquisition unit; and a contrast adjusting portion that adjusts, for the respective channels, contrasts of the images acquired by the image-acquisition unit based on the contrast adjusting methods stored in the adjustment-method storage portion.

A transmission spectroscopy device can direct light into a sample, and determine properties of the sample based on how much light emerges from the sample. The device can use a cell to contain the sample, so that the size of the cell defines the optical path length traversed by light in the sample. To ensure accuracy in the measurements, it is beneficial to calibrate the device by measuring the size of the cell periodically or as needed. To measure the size of the cell, the device can perform a transmission spectroscopy measurement of a known substance, such as pure water, to produce a measured absorbance spectrum of the known substance. The device can subtract a known absorbance spectrum of the known substance from the measured absorbance spectrum to form an oscillatory fringe pattern. The device can determine the size of the cell from a period of the fringe pattern.

Apparatus and method for obtaining a plurality of spectral images of a source object in a snapshot using comprising two-dimensional compressed sensing data cube reconstruction (2D CS-SCR) applied to a dispersed-diffused snapshot image. In some embodiments, the snapshot image is obtained through a RIP diffuser. In some embodiments, a randomizer is used to further randomized the dispersed-diffused snapshot image. The 2D CS-SCR includes applying a 2D framelet transform separately to arrays representing different wavebands of spectral cube data derived from the snapshot image. The application of the 2D framelet transform separately to the arrays representing the different wavebands includes application of direct and inverse 2D framelet transforms to the arrays. In some embodiments, the direct and inverse framelet transforms are included in a split Bregman iteration.

A multichannel color measurement instrument for measuring spectral properties of a target comprises pick-up optics to collect measurement light, first and second anamorphic optical paths optically coupled to the pick up optics, a pick-up polarizing element located to polarize measurement light in the second anamorphic optical path, a reference anamorphic optical path including a reference illumination source, and a two-dimensional variable filter sensor having an optically transmissive filter function that varies in a first direction parallel to a surface of the variable filter sensor and is substantially constant in a second direction parallel to a surface of the variable filter sensor and orthogonal to the first direction. The anamorphic optical paths spread the measurement light in the first direction direct it on to different portions of the variable filter sensor.

A method, a system, and a non-transitory computer readable medium for Raman spectroscopy. The method may include determining first acquisition parameters of a Raman spectroscope to provide a first acquisition set-up, the determining is based on at least one expected radiation pattern to be detected by a sensor of the Raman spectroscope as a result of an illumination of a first area of a sample, the first area comprises a first nano-scale structure, wherein at least a part of the at least one expected radiation pattern is indicative of at least one property of interest of the first nano-scale structure of the sample; wherein the first acquisition parameters belong to a group of acquisition parameters; setting the Raman spectroscope according to the first acquisition set-up; and acquiring at least one first Raman spectrum of the first nano-scale structure of the sample, while being set according to the first acquisition set-up

A mode of a spectrometer according to the present invention includes a spectrum measurement unit (11, 12) configured to repeatedly measure a spectrum over a predetermined wavelength range for measurement target light that is laser light; a peak counting unit (21, 22) configured to, every time a spectrum is obtained by the spectrum measurement unit, detect a peak from the spectrum and count the number of detected peaks; and a display processing unit (24) configured to display a numerical value of a peak counting result by the peak counting unit on a screen of a display unit in real time. With the spectrometer of the above mode, adjustment and the like of the multimode laser oscillator can be efficiently and accurately performed.

A surgical access assembly comprises a trocar and a surgical instrument. The trocar comprises a housing and an access tube extending distally from the housing. The housing comprises a hollow light emitter. The housing and the access tube define a lumen extending through the housing and the access tube. The hollow light emitter is configured to project light in the lumen. The surgical instrument comprises an end effector and a shaft extending proximally from the end effector. The shaft comprises an optical receiver positioned within reach of the light from the hollow light emitter. The shaft further comprises a light guide extending from the optical receiver along at least a portion of the shaft toward the end effector.

A Brillouin modality can be supplemented by an auxiliary modality, such as an optical imaging modality or a spectroscopy modality. In some embodiments, the auxiliary modality can be used to guide the Brillouin measurement to a desired region of interest, so that acquisition times for the Brillouin measurement can be reduced as compared to interrogating the entire sample. The auxiliary modality may have an acquisition speed faster than that of the Brillouin modality. In some embodiment, the auxiliary modality determines a composition of materials within a voxel in the sample interrogated by the Brillouin modality. Using the information provided by the auxiliary modality, the Brillouin signatures corresponding to the materials within the voxel can be unmixed, thereby providing a more accurate measurement of the sample.

Disclosed are an optical spectroscopy system using a matched filter-based broadband signal receiver for stable data extraction, and a method for controlling the optical spectroscopy system. The optical spectroscopy system may comprise: a light transmission unit for irradiating light on a particular region of a subject by means of a plurality of light sources, wherein the light irradiated from the plurality of light sources is code-modulated by means of the Walsh codes and then irradiated; and a light receiving unit for detecting emergent light which has passed through the particular region, wherein the light source is identified by demodulating the light by means of the Walsh codes.