A system includes a light source, a transparent container, a detector, and a processor. The light source emits light. Liquid flows from one end of the transparent container to another end of the transparent container. The liquid comprises a first and a component. One or more droplets containing the second component are formed within the transparent container as the liquid flows from the one end to the another end of the transparent container. The detector measures light intensities from the transparent container being illuminated. The one or more droplets cast shadows on the detector. A light intensity associated with a portion of the liquid that includes the one or more droplets is different from a second light intensity associated with another portion of the liquid that does not include the one or more droplets. The processor processes the measured light intensities to determine phase entrainment metrics associated with the liquid.

Devices, techniques, and processes are disclosed that use electrical impedance to detect of the presence and contents of droplets including cells, nucleic acids, proteins, or solute concentrations in an array of retrievable, trackable, trapped droplets in a fluidic system. Electrodes may be positioned underneath individual droplet traps in a microchannel to assay droplet contents and/or actuating droplets for the release of the droplets from corresponding traps. The disclosed technology may be used for detection of the results of solvent extraction processes including time-dependent quantification of metal ion concentration in the aqueous and organic phases, for wastewater treatment, heavy metal detection, pharmaceutical industry, and/or biotechnology, or for environmental monitoring of wastewater for toxic metal, monitoring of biological cell viability and proliferation, monitoring of extraction processes used in heavy metal mining, monitoring of extraction processes used in nuclear fuel processing, monitoring kinetics of enzyme processes, and/or assessing pharmacodynamics and drug efficacy.

Systems, methods, and computer-program products for fluid analysis and monitoring are disclosed. Embodiments include a sampling system and an analytical system connected to the sampling system. A fluid may be routed through the sampling system and data may be collected from the fluid via the sampling system. The sampling system may process and transmit the data to the analytical system. The analytical system may include a command and control system to receive and store the data in a database and compare the data to existing data for the fluid in the database to identify conditions in the fluid. The system may further include a cooling system configured to enclose at least one member of the fluid analysis system. The cooling system encloses at least one member of the fluid analysis system including the excitation system, the detection system, the fluid inlet, the sample chamber, the Raman probe, and combinations thereof.

Devices, techniques, and processes are disclosed that use electrical impedance to detect of the presence and contents of droplets including cells, nucleic acids, proteins, or solute concentrations in an array of retrievable, trackable, trapped droplets in a fluidic system. Electrodes may be positioned underneath individual droplet traps in a microchannel to assay droplet contents and/or actuating droplets for the release of the droplets from corresponding traps. The disclosed technology may be used for detection of the results of solvent extraction processes including time-dependent quantification of metal ion concentration in the aqueous and organic phases, for wastewater treatment, heavy metal detection, pharmaceutical industry, and/or biotechnology, or for environmental monitoring of wastewater for toxic metal, monitoring of biological cell viability and proliferation, monitoring of extraction processes used in heavy metal mining, monitoring of extraction processes used in nuclear fuel processing, monitoring kinetics of enzyme processes, and/or assessing pharmacodynamics and drug efficacy.

Systems and method for sorting droplets includes a microfluidic chip or substrate having a droplet sorting channel coupled at an upstream location to a droplet inlet channel, the droplet sorting channel coupled at a downstream location to a waste channel and a collection channel. The device includes an optical interrogation device configured to illuminate the droplets passing through the sorting channel with excitation light from an excitation light source and capturing emitted fluorescent light and generating an output signal correlated to the fluorescence of the droplets. An actuator (electrode) is disposed in the microfluidic chip or substrate and coupled to a signal driver (e.g., a high voltage amplifier). The device or system uses a programmable controller configured to receive the output signals from the optical interrogation device and trigger the signal driver to actuate the actuator to direct the droplets into the collection channel.

The present invention provides methods and devices for detecting and quantifying multiple biomolecules at single-molecule level using an integrated droplet microfluidic system. In one embodiment, the present invention provides real-time and digital measurement of multiple biomolecules in a sample, thereby quantifying multiple biomolecules in an absolute and simultaneous manner. In one embodiment, the present invention provides a diagnostic method for a disease, comprising real-time and digital measurement of multiple biomolecules in a sample using the method or device described herein.

An apparatus to detect contaminants in a fuel comprises an input to receive a fuel flow. A light scattering system is coupled to the input. An imaging system is coupled to the light scattering system. A memory is coupled to the imaging system. A processor is coupled to the memory. Output signals from the imaging and light scattering systems are transferred to the processor. The processor is configured to cause the light scattering system to monitor the light scattering intensity from the contaminants in the fuel flow. The processor is configured to cause the light scattering system to measure a light scattering intensity signal from the contaminants in the fuel flow. The processor is configured to generate a trigger signal to turn on the imaging system when the light scattering intensity is greater than a predetermined threshold.

A microfluidic device for performing physical, chemical or biological treatment to at least one packet without contamination.

The present invention discloses a system for detecting the concentration of non-metal particles in a fluid and detection method thereof. The detection system comprises a particle morphology detection device, a metal particle detection device, and a detection pipeline, the particle morphology detection device and the metal particle detection device being connected to each other and wound around the detection pipeline. The detection method comprises: S1, detecting the concentration of fluid particles; S2, detecting the concentration of fluid metal particles; and S3, detecting concentration of fluid non-metal particles. By means of the detection system and the detection method, the concentration of non-metal particles in a fluid can be more accurately detected, and the detection accuracy is improved.

Metal oxide gel particles, may be prepared with a desired particle size, by preparing a low-temperature aqueous metal nitrate solution containing hexamethylene tetramine as a feed solution; and causing the feed solution to flow through a first tube and exit the first tube as a first stream at a first flow rate, so as to contact a high-temperature nonaqueous drive fluid. The drive fluid flows through a second tube at a second flow rate. Shear between the first stream and the drive fluid breaks the first stream into particles of the metal nitrate solution, and decomposition of hexamethylene tetramine converts metal nitrate solution particles into metal oxide gel particles. A metal oxide gel particle size is measured optically, using a sensor device directed at a flow of metal oxide gel particles within the stream of drive fluid. The sensor device measures transmission of light absorbed by either the metal oxide gel particles or the drive fluid, so that transmission of light through the drive fluid changes for a period of time as a metal oxide gel particle passes the optical sensor. If a measured particle size is not about equal to a desired particle size, the particle size may be corrected by adjusting a ratio of the first flow rate to a total flow rate, where the total flow rate is the sum of the first and second flow rates.