Systems and methods analyzing body fluids contain cells including blood, bone marrow, urine, vaginal tissue, epithelial tissue, tumors, semen, and spittle are disclosed. The systems and methods utilize an improved technique for applying a monolayer of cells to a slide and generating a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and method for utilizing multi-color microscopy for improving the quality of images captured by a light receiving device.

Among other things, the present invention is related to devices/apparatus and methods of performing cellular, biological, and chemical assays and procedures.

An alarming method for a platelet aggregation sample can include providing a blood sample, preparing a first test sample from the blood sample under a first reaction condition, acquiring a test signal of the first test sample, and obtaining first platelet test data. The method can also include preparing a second test sample from the blood sample under a second reaction condition, acquiring a test signal of the second test sample, and obtaining second platelet test data. The method can further include obtaining an evaluation result based on the first platelet test data and the second platelet test data, determining whether the evaluation result meets a predetermined condition, and alarming that the blood sample may be the platelet aggregation sample if the evaluation result meets the predetermined condition. The second reaction condition may include a reaction condition for reducing the platelet aggregation degree of the blood sample.

Aspects of the present disclosure include methods for detecting events in a flow cytometer. Also provided are methods of detecting cells in a flow cytometer. Other aspects of the present disclosure include methods for determining a level of contamination in a flow cell. Computer-readable media and systems, e.g., for practicing the methods summarized above, are also provided.

A technique is presented for aligning, in a desired region within a flow chamber of a flow cell, a non-spherical biological entity carried in a sample. The flow chamber has a rectangular cross-section. A bottom flow input module, a top flow input module and a sample input module provide a viscoelastic first fluid, a second viscoelastic fluid, and the sample, respectively, to the flow chamber. The first and the second viscoelastic fluids laminarly flow along a bottom and a top wall of the flow chamber and the sample laminarly flows sandwiched between them. By controlling rate of flow of the first and/or the second viscoelastic fluids the sample flow, and thus the non-spherical biological entity, is focused in the desired region. A gradient of sheer within the sample flow set up due to the first and second viscoelastic fluids orients the non-spherical biological entity in the desired region.

Aspects of the present disclosure include methods for detecting events in a flow cytometer. Also provided are methods of detecting cells in a flow cytometer. Other aspects of the present disclosure include methods for determining a level of contamination in a flow cell. Computer-readable media and systems, e.g., for practicing the methods summarized above, are also provided.

Devices for detecting particle sizes and distributions using focused light scattering techniques, by passing a sample through a focused beam of light, are disclosed. In one embodiment, the devices include one or more lasers, whose light is focused into a narrow beam and into a flow cell, and dispersions are passed through the flow cell using hydrodynamic sample injection. In another embodiment, a plurality of lasers is used, optionally with hydrodynamic sample injection. Particles pass through and scatter the light. The scattered light is then detected using scatter and extinction detectors, and, optionally, fluorescence detectors, and the number and size of the particles is determined. Particles in the size range of 0.1 to 10 .Math. can be measured. Using the device, significantly smaller particles can be detected than if techniques such as EQELS, flow cytometry, and other conventional devices for measuring biological particles.

The disclosure relates to devices and methods for analyzing blood cells in a sample. In various embodiments, the present disclosure provides devices and methods of performing complete blood count (CBC) testing. In various embodiments, the present disclosure provides a cartridge device and a reader instrument device, wherein the reader instrument device receives, operates, and/or actuates the cartridge device. In various embodiments, the present disclosure provides a method of using a device as disclosed herein for analyzing blood cells in a sample.

A blood detection method and a blood detection device are disclosed. When a count number of platelet in a blood sample is less than a predetermined value, a detection solution of the blood sample is prepared. A cell statistical amount of the detection solution of the blood sample is increased to obtain the platelet detection result. The cell statistics amount of the detection solution of the blood sample may be increased in an impedance detection area to perform PLT-I detection, or in an RET detection area; or performing both of RET detection and PLT-O detection simultaneously. Thus, the accuracy of platelet detection can be improved when the number of platelets in the blood sample is low.

The disclosure relates to devices and methods for analyzing particles in a sample. In various embodiments, the present disclosure provides devices and methods for cytometry and additional analysis. In various embodiments, the present disclosure provides a cartridge device and a reader instrument device, wherein the reader instrument device receives, operates, and/or actuates the cartridge device. In various embodiments, the present disclosure provides a method of using a device as disclosed herein for analyzing particles in a sample.