Diagnostic and screening technologies, therapy recommendations, and computer systems based on red blood cell membrane permeability characteristics are provided herein.

Methods and devices for electrochemically controlled degradation of metals particles using halide biochemistry allowing in-situ control and being suitable for analyte detection and quantification.

The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.

A system and method to measure a size distribution of particles based on their electrical mobility. The method includes: introducing, via a sheath flow inlet, a particle free sheath flow into a chamber formed by two parallel walls which are separated by a gap, the chamber having a width and a length, the sheath flow having a direction along the length of the chamber and flowing a laminar manner; introducing an aerosol sample flow into the chamber downstream of the sheath inlet such that the aerosol sample flow joins the particle free sheath flow in a laminar manner; applying an electric field between the two parallel walls of the chamber, the field having a strength which varies across the width of the chamber; extracting an output aerosol flow through a first outlet downstream of the sample inlet; and outputting an excess flow equal to a sum of the sheath flow and aerosol sample flow minus the output aerosol flow. The method may also include: passing the output aerosol flow through a growth cell in a laminar manner, the growth cell having a region of wetted walls with two or more temperature regions such that the particles within the output aerosol flow grow by condensation to form droplets, and such that relative positions of droplets are indicative of particle electrical mobility; and counting and capturing a spatial position of individual droplets exiting the growth cell.

A uniformity output device for outputting a uniformity of particles in a slurry, in which an insoluble solid matter is mixed in liquid, includes: a pair of electrodes configured to apply AC voltage to the slurry; a measurement unit configured to measure impedance of the slurry on the basis of the response current flowing through the slurry when AC voltage with changing frequency is applied to the slurry; and a processing unit configured to determine the uniformity by executing a particle equivalent circuit analysis with a parallel circuit formed of a resistor and a capacitor as an element on the basis of the impedance measured by the measurement unit in accordance with the frequency.

A method of monitoring one or more cell aggregates, comprising providing a flow path in which the one or more cell aggregates are in a medium and the flow path being configured to pass through a collective sensing zone of a set of electrodes, obtaining impedance-related signals corresponding to each of the medium and one of the one or more cell aggregates in the medium, determining one or more electrical signatures for a cell aggregate, in which the one or more electrical signatures are based on impedance-related signals obtained from the set of electrodes. The method is one of dynamic testing at single-particle resolution. The electrical signatures may be an opacity and/or electrical size of the one or more cell aggregates, or electrical impedance spectroscopy-based electrical signatures. The cell aggregate is a spheroid, an encapsulated microcarrier, or a cell-adhered microcarrier. It is also to provide a microfluidic chip comprising a channel and electrodes for obtaining impedance- related signals.

A particle detecting device is provided. The particle detecting device includes a resonator and a piezoelectric actuator. The piezoelectric actuator is used to transport a gas into the resonator, and a mass and a concentration of the screened and required-diameter particles are detected through the resonator. Thus, the air quality can be monitored immediately anytime and anywhere.

Provided are a device and a method for rapidly and simply detecting endotoxin without using an expensive reagent. The endotoxin detection device includes: a region containing an electrolyte solution; a partitioning member that partitions the region into two compartments such that the two compartments are in communication via a nanopore; a first electrode that is disposed in a first compartment; a second electrode that is disposed in a second compartment and is electrically connected to the first electrode; an electrolyte solution flow generating means that causes electrolyte solution in the first compartment to move to the second compartment via the nanopore; an application means that applies voltage between the first electrode and the second electrode; and a monitoring means that monitors current.

A method is provided for storing a particle analyzer capable of suppressing deterioration of a measurement performance with the lapse of time in a particle analyzer for analyzing particles such as exosomes, pollen, viruses, and bacteria. The particle analyzer has a first storage chamber in which a first liquid is stored, a second storage chamber in which a second liquid containing particles to be analyzed is stored, and a flow path connecting the first storage chamber in fluid communication with the second storage chamber. According to the method, at least a portion of the first storage chamber, the second storage chamber, and the flow path are surface-treated, which includes filling an internal space defined by the first storage chamber, the second storage chamber, and the flow path with a liquid to thereby store the particle analyzer in a state that the surface-treated portion is not in contact with air.

A nanopore device includes a pore and an electrode pair. A current measurement unit applies a bias voltage that corresponds to a voltage setting command across an electrode pair and generates digital current data that corresponds to a current signal that flows through the nanopore device. A data processing apparatus generates the voltage setting command, acquires the current data and voltage data including information with respect to the waveform of the bias voltage Vb in a form in which they are associated on the time axis, and judges the kind of particles stored in the nanopore device based on the current data and the voltage data.