A method and device for analyzing a hematologic sample centrifuged within a capillary tube is provided. The device includes a tube holder, a sample imaging device, a processor, and a sample data display. The sample imaging device is operable to create a digital image of the sample within a region of the tube. The region is defined by substantially all of the radial width and axial length of the sample residing within the internal cavity of the tube in the region where the float resides after centrifugation. The sample imaging device is operable to produce signals representative of the image. The processor is adapted to produce information relating to bands of interest within the image based on the signals from the sample imaging device. The sample data display is adapted to display the results therefrom and/or a digital image of the sample within the region.

A sample holder for use in a centrifuge, the sample holder being generally planar and comprising: an aperture or recess for releasably retaining a sample storage member including a sample chamber adapted to contain a volume of liquid; a centre point around which the holder will rotate during use; and one or more calibration features, wherein the calibration feature(s) comprise one or more outer edges, which lie on the side of the or each calibration feature which is furthest from the centre point, and the one or more outer edges comprise a series of radially spaced-apart outer edge portions or positions which are spaced at different distances from the centre point as a function of angular position around the centre point.

An imaging system for a rotatable object includes an imaging unit configured to take a series of images of a portion of the rotatable object and a light source. The light source is directed at the rotatable object and is configured to generate pulses of light that illuminate the rotatable object during rotation of the rotatable object and allow the imaging unit to take the series of images of the rotatable object. The system also includes a synchronizer that monitors the rotational position of the rotatable object as it rotates, and a controller in communication with the imaging unit, the light source, and the synchronizer. The controller controls the operation of the imaging unit and/or the light source based upon the rotational position of the rotatable object such that each of the series of images is taken at the same rotational position of the rotatable object.

A method and system for processing particles contained in a liquid biological sample is presented. The method uses a rotatable vessel for processing particles contained in a liquid biological sample. The rotatable vessel has a longitudinal axis about which the vessel is rotatable, an upper portion having a top opening for receiving the liquid comprising the particles, a lower portion for holding the liquid while the rotatable vessel is resting, the lower portion having a bottom, and an intermediate portion located between the upper portion and the lower portion, the intermediate portion having a lateral collection chamber for holding the liquid while the rotatable vessel is rotating. The method employs dedicated acceleration and deceleration profiles for sedimentation and re-suspension of the particles of interest.

Means and methods utilizing a combination of bioelectrical stimulator and platelet-rich fibrin for accelerated tissue or wound healing and regeneration is described. The system bioelectrically stimulates the centrifuge, test tube, and/or subject to produce enhanced levels of, e.g., SDF, PDGF, HGF, VEGF, IGF, Sonic hedgehog, klotho, and/or tropoelastin. The described system produces much higher levels of regenerative proteins delivered over an extended period of time.

Provided is a density gradient liquid production apparatus for producing a density gradient liquid in a measurement cell for a centrifugal sedimentation type particle diameter distribution measurement device and comprises a first solution preparation unit that prepares a first solution with a predetermined target concentration and a first density by mixing a plurality of first reference solutions each of whose concentrations of an agglomeration inhibiting component that inhibits agglomeration of particles differs, and a second solution preparation unit that prepares a second solution with the predetermined target concentration and a second density that is different from the first density by mixing a plurality of second reference solutions each of whose concentrations of the agglomeration inhibiting component differs, and a mixed solution preparation unit that creates a mixed solution with the target concentration by mixing the first solution and the second solution and supplies the created mixed solution to the measurement cell while varying a density of the mixed solution.

A sample liquid-surface position measurement device includes: a first light source that illuminates a side face of a container containing a sample; a first optical measurement sensor that is located on the opposite side of the container from the first light source, and measures transmitted light from the first light source; a first label position measuring unit that measures a position of a label affixed to the container; and an analysis section that calculates a liquid-surface position or an interface position of the sample in the container, from transmitted-light intensity data in a longitudinal direction of the container which is measured by the first optical measurement sensor, and from the position of the label in the longitudinal direction of the container which is measured by the first label position measuring unit.

A centrifugal field-flow fractionation device capable of improving analysis performance and shortening analysis time is provided. A first channel 111 communicating with a channel member is formed on a rotational shaft 11 that rotates together with a rotor. A second channel 644 communicating with the first channel 111 is formed on a fixing portion 60 fixed in a state of facing the rotational shaft 11 along a rotational axis L. A mechanical seal 66 having a pair of seal rings 661 and 662 that come into contact with each other and a biasing member 663 is provided to attach one seal ring 661 to the rotational shaft 11 and the other seal ring 662 to the fixing portion 60. The biasing member 663 biases the pair of seal rings 661 and 662 in a direction in which the pair of seal rings come in contact with each other. Since the rotational shaft 11 can be rotated at a high speed and the liquid sample can be fed at a high pressure, the analysis performance can be improved and the analysis time can be shortened.

This optical cell for sedimentation analysis has a pair of opposing surfaces through which light is transmitted, and two polarizing plates, in which each of the two polarizing plates is disposed in a crossed Nicols state on each of the inner surfaces of the pair of opposing surfaces.

Provided herein are methods to characterize preparations of recombinant viral particles using analytical ultracentrifugation. Recombinant viral particles include recombinant adeno-associated viral particles, recombinant adenoviral particles, recombinant lentiviral particles and recombinant herpes simplex virus particles. Variant species of recombinant viral particles including empty capsids and recombinant viral particles with variant genomes (e.g., truncated genomes, aggregates, recombinants) can be identified and quantitated. The methods can be used to characterize preparations of recombinant viral particles regardless of the sequence of the recombinant viral genome or the serotype of the recombinant viral capsid.