A system for selective microcapsule extraction includes a non-planar core-shell microfluidic device. The non-planar core-shell microfluidic device generates microcapsules defining a core-shell configuration. A subset of the microcapsules contain aggregates, tissues, or at least one cell. A camera captures images of the microcapsules. A detection module includes a processor and a memory. The memory includes instructions that when executed by the processor causes the detection module to provide the images of the microcapsules as an input to a machine learning model. The machine learning model identifies microcapsules containing aggregates, tissues, or at least one cell. A force generator generates a force to extract the microcapsules. A microcontroller selectively activates the force generator to generate the force when the detection module identifies a microcapsule containing aggregates, tissues, or at least one cell to extract the microcapsule.

The present invention relates to a method for screening an antibody or antigen-binding fragment thereof by using cells bearing magnetic beads and, more particularly, to a method for screening an antibody binding specifically to an antigen protein or an antigen-binding fragment thereof, in which cells having biotinylated phospholipids in the cell membranes thereof and a streptavidin-magnetic bead complex fused to the surfaces thereof, and a magnetic-based system are utilized.

Systems having an unfiltered light scatter detector configured to detect scattered light from a sample in a flow stream are provided. Systems according to certain embodiments include a light source having two or more lasers, a light detection system having an unfiltered light scatter detector and a processor having memory operably coupled to the processor where the memory includes instructions which when executed by the processor, cause the processor to generate one or more data signals in response to scattered light from each of the two or more lasers detected by the unfiltered light scatter detector; and determine one or more parameters of data acquisition based on the generated data signals from the unfiltered light scatter detector. Methods for determining one or more parameters for data acquisition with the subject systems are also described.

This disclosure provides an impedance cytometer which includes a carrier that can be attached to a living being, with a biosensor mounted thereto. The bio sensor includes a microfluidic flow channel, formed in the carrier, and an impedance circuit. The microfluidic flow channel accommodates passage of a particle therethrough. The impedance circuit, connected to the microfluidic flow channel, includes a signal generator that produces a high-frequency drive signal applied to the flow channel to produce a biosensor output signal having high-frequency variation resulting from the drive signal and low-frequency variation resulting from impedance variation within the flow channel during the particle's passage. A lock-in amplifier is disposed to (i) amplify the bio sensor output signal, (ii) mix the amplified signal with the drive signal, and (iii) frequency-filter the mixed, amplified signal to output an impedance signal representing the low-frequency impedance variation resulting from the passage of the particle. Embodiments enable wearable, personalized cytometry.

An optical measurement system comprises a polarization beam splitter for dividing an incident beam into a reference beam and a measurement beam, a first beam splitter for reflecting the measurement beam to form a first reflected measurement beam, a spatial light modulator for modulating the first reflected measurement beam to form a modulated measurement beam, a condenser lens for focusing the modulated measurement beam to an object to form a penetrating measurement beam, an objective lens for converting the penetrating measurement beam into a parallel measurement beam, a mirror for reflecting the parallel measurement beam to form an object beam, a second beam splitter for reflecting the reference beam to a path coincident with that of the object beam, and a camera for receiving an interference signal generated by the reference beam and the object beam to generate an image of the object.

Methods for classifying fluorescent flow cytometer data are provided. In some instances, methods include processing the flow cytometer data with a supervised algorithm configured to cluster the fluorescent flow cytometer data into distinct populations according to the relationship of data points to relevant threshold values. In embodiments, methods include determining a measure of spillover spreading by calculating spillover spreading coefficients and combining them in a spillover spreading matrix. In some embodiments, populations of fluorescent flow cytometer data are adjusted to subtract the magnitude of spillover spreading. In embodiments, spillover spreading adjusted populations are partitioned after potential partitions are evaluated relative to the threshold values. In embodiments, partitioned populations of fluorescent flow cytometer data are classified (i.e., phenotyped) according to a hierarchy. Systems and computer-readable media for classifying fluorescent flow cytometer data are also provided.

A urine analysis system according to an embodiment includes: a testing apparatus that measures particles included in a urine sample according to a flow cytometry method; an image capturing apparatus that captures images of particles in the urine sample to acquire particle images; and a management apparatus that receives a measurement result obtained by the testing apparatus and the particle images acquired by the image capturing apparatus. The management apparatus generates an order to capture an image of the urine sample based on the measurement result obtained by the testing apparatus. The image capturing apparatus executes the image capturing processing of the particles in the urine sample for which the image capturing order has been generated by the management apparatus, and transmits the acquired particle images to the management apparatus.

To provide a technology of optimizing a suction condition of a microparticle.

The present technology provides an optimizing method of a suction condition of a microparticle including: a particle number counting step of detecting a time point when a microparticle passes through a predetermined position on a main flow path through which liquid containing the microparticle flows, sucking the microparticle from the main flow path to a microparticle suction flow path by the microparticle suction flow path with a predetermined suction force, and counting the number of microparticles sucked into the microparticle suction flow path; and a step of determining an elapsed time from passage through the predetermined position with which the suction by the microparticle suction flow path should be performed on the basis of a time from the time point when the microparticle passes through the predetermined position on the main flow path until the suction is performed and the number of counted microparticles.

Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.

A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.