Methods and systems for determining interaction between cells are described wherein the method includes determining or receiving a sequence of images representing manipulating first cells, in a holding space, the holding space including a functionalized wall comprising second cells, the manipulating including settling of the first cells onto the functionalized wall and applying a force on the settled first cells; detecting groups of pixels representing first cells in first images representing the settling of the first cells onto the functionalized wall; tracking locations of detected first cells in the first images; and, determining settling events, a settling event being determined if a cell in a first image is not distinguishable from background of the first image, the location in the image at which a cell settling event is detected defining a cell settling location; detecting groups of pixels representing cells in second images captured during the application of the force and tracking locations of detected cells, wherein tracked locations of a detected cell in the second images form a tracking path, the first location of the tracking path defining a pop-up event, the location in a second image at which a pop-up event is detected defining a pop-up location; and, determining detachment events based on the settling locations and based on the pop-up locations, a detachment event defining a first cell being detached from a second cell due to application of the force on the first cell, and determining information about the interaction between first and second cells based on the force applied to the first cells.

A fluorescence image analyzer has an imaging unit for capturing a first image containing at least a part of a region of a cell as an imaging target for a plurality of cells in a sample in which a target site on a chromosome is labeled with a fluorescent dye, and a second image including fluorescence generated from a fluorescent dye labeling the target site of the cell of the first image. The processing unit selects a plurality of test cells having specific morphological characteristics to be tested from a plurality of cells based on at least the first image, and extracts the bright spots of fluorescence generated from the fluorescent dye. The processing unit identifies cells with chromosomal abnormalities and/or cells without chromosomal abnormalities based on the extracted bright spots, and generates information related to the ratio of cells with chromosomal abnormalities relative to the test cells.

A method and system for studying cell viability and protein aggregation. In one aspect, the method relies on speckle information to analyze cells or other structures present in a fluid. A probe for measuring in situ structures includes a tip section having a sample detection region and a camera provided with image sensors.

High mitochondrial ATP is a metabolic trait that confers hyper-proliferation, sternness, anchorage-independence, anti-oxidant capacity and multi-drug resistance in cancer cells. Under the present approach, intracellular ATP levels may be used as a metabolic biomarker to identify, separate, and purify an aggressive and hyper-proliferative cancer stem cell (“CSC”) phenotype. Further, ATP may be combined with other CSC markers, e.g., CD44 or ALDH-activity, to beneficially fractionate the CSC population into sub-populations. For example, ATP-high/ CD44-high CSC sub-populations showed twice the level of anchorage-independent growth compared to ATP-low/CD44-high CSC sub-populations. Also disclosed are complementary bioinformatic data that implicate mitochondrial ATP synthesis in stemness, metastasis, and the detection of circulating tumor cells (“CTCs”), and a five-member, ATP-related metastasis gene-signature (ABCA2, ATP5F1C, COX20, NDUFA2 and UQCRB). The gene signature of the present approach may be used to identify CSCs having a dramatic increase in cell migration and invasion in vitro capacity, as well as spontaneous metastasis in vivo. The present approach also provides a cellular platform for systematically targeting sternness, multi-drug resistance, and metastasis in cancer cells.

Embodiments of the present invention provide predictions from semen qualities (7) embryo characteristics (9), qualities (11), intracellular qualities (13), extracellular qualities (14), or the like of which a computational device prediction models automated computational transformation algorithm (3) may be applied to create a prediction model transformed data (4) perhaps to generate a prediction models completed prediction output which may be used to predict parameters (6) such as fertility-related parameters, fertility of an animal, embryo success rate, or the like.

Disclosed is a method for processing Raman data of eosinophil based on artificial intelligence, the method being executed by a device, the method including generating Raman data by performing Raman analysis using a specific wavelength on eosinophils isolated from blood of a diagnosed person, pre-processing the generated Raman data, assigning a weight to the pre-processed Raman data for each of components including a nucleus, a cell membrane, a granule, and a background, classifying data for each component based on a result of assigning the weight, extracting data in which the component is the granule based on a classified result; and determining whether a specific disease has occurred in the diagnosed person through eosinophil characteristics of the diagnosed person based on the extracted data.

A light source apparatus can avoid double-counting of particles in a flow cytometer for measuring and analyzing a plurality of particles flowing in a flow cell. A light source apparatus for a flow cytometer includes a semiconductor laser for emitting a laser beam, a collimating lens for collimating the laser beam emitted from the semiconductor laser in a spread light state, a first beam conversion unit composed of prisms and a second beam conversion unit composed of prisms for matching a flow cell length direction with a slow axis direction of the collimated laser beam in a flow cell after reducing the beam diameter in a fast axis direction and increasing the beam diameter in the slow axis direction, and a focusing lens for focusing the laser beam passed through these beam conversion units in the flow cell.

Particle analyzers having scintillation counters are provided. Particle analyzers of interest include a flow cell for transporting particles in a flow stream, a light source for irradiating a particle in the flow stream at an interrogation point, a particle-modulated light detector for detecting light from the interrogation point, and a scintillation counter for assessing particle radioactivity. In embodiments, the scintillation counter is positioned within the flow cell and configured generate particle radioactivity data that may be associated with a given particle in a plurality of particles. Methods and non-transitory computer readable storage media for practicing the invention are also provided.

Aspects of the present disclosure include systems for detecting light from a particle by birefringent interferometry. Systems according to certain embodiments include a light source configured to irradiate a particle propagating through a flow stream, a light detection system that includes a birefringent polarizing interferometer configured to generate interfering polarized beams of light, a light adjustment component configured to continuously convey light from the irradiated particle across different positions on the birefringent polarizing interferometer as the particle is propagated through the flow stream, a photodetector configured to detect interference patterns of the interfering polarized beams of light generated by the birefringent polarizing interferometer from light collected from the irradiated particle and generate a photodetector signal pulse in response to each detected interference pattern. Systems also include a processor for transforming the photodetector signal pulses into spectral data signals. Methods for detecting light with the subject systems are also described. Kits having one or more components for detecting light according to the subject methods are also provided.

Aspects of the present disclosure include systems for detecting light from a particle in a flow stream by spectral discrimination. Systems according to certain embodiments include a light source configured to irradiate a particle propagating along a flow stream through an interrogation region, a light detection system that includes a wavelength separator component configured to pass light having a predetermined spectral range across the wavelength separator, a light adjustment component configured to continuously convey light from the irradiated particle across the wavelength separator as the particle is propagated along the flow stream through the interrogation region and a photodetector configured to detect light conveyed across the wavelength separator. Systems also include a processor for generating a photodetector signal pulse in response to light detected from the wavelength separator. Methods for detecting light with the subject systems are also described. Kits having one or more components for detecting light according to the subject methods are also provided.