A calibration set includes first nanoparticles comprising nylon-6 covalently bound to a first dye, the first nanoparticles having a first average diameter and a first polydispersity index of greater than or equal to 1.15 and less than or equal to 1.19. The calibration set further includes second nanoparticles comprising nylon-6 covalently bound to a second dye that is different from the first dye, the second nanoparticles having a second average diameter and a second polydispersity index of greater than or equal to 1.15 and less than or equal to 1.19. The first average diameter is different from the second average diameter, the first average diameter and the second average diameter are each independently greater than or equal to 30 nm and less than or equal to 3000 nm, and the first average diameter is at least two times the second average diameter.

A particle analysis system comprising: a light detector that acquires light generated by irradiating a particle with excitation light; and an information processing unit that outputs a spectral plot including spectrum information of an autofluorescence population specified in a two-dimensional plot of measurement data each of which corresponds to the acquired light and spectrum information of the measurement data and that records the spectrum information of the autofluorescence population as an autofluorescence reference spectrum in a fluorescence separation process.

A sensor for particle identification, the sensor comprising: a first chamber configured to be filled with an electrolytic solution; a first electrode provided inside the first chamber and configured to be connected to an external power supply for applying a voltage; a second chamber configured to be filled with the electrolytic solution; a second electrode provided inside the second chamber and configured to be connected to the external power supply; a data output means configured to output measurement data expressing an ion current generated between the first electrode and the second electrode; a partition separating the first chamber and the second chamber; and a presentation means for providing a unique identifier to an external computer device over a network. The partition includes a pore connecting the first chamber and the second chamber, a physical property of the sensor is associated with the unique identifier, the sensor is configured such that when a particle passes through the pore, a transient change dependent on at least a physical property of the pore and a physical property of the particle occurs in the ion current generated between the first electrode and the second electrode, and the unique identifier is configured to cause the external computer device receiving the unique identifier to perform a process of identifying the particle according to the physical property of the sensor associated with the unique identifier. The physical property of the sensor at least includes a physical property of the pore.

The invention relates to a method for detecting particles, having the steps of: receiving (S1) a measurement signal; calculating (S2) at least one estimated noise value using the received measurement signal; and detecting (S3) the particles using the measurement signal on the basis of at least one detection criterion, wherein the at least one detection criterion depends on the at least one calculated estimated noise value.

Hydrogel particles and their use in cytometric applications are described. The hydrogel particles described herein are selectively tunable to have at least one optical property substantially similar to at least one optical property of a target cell. In this regard, the hydrogel particles provided herein, in one aspect, are used as a calibration reagent for the detection of a target cell in a sample.

A method includes calibrating a cytometric device for analysis of a target cell, by inserting, into the cytometric device, a hydrogel particle. The hydrogel particle has at least one of a background fluorescent property or a spectral property that is substantially similar to the at least one of a background fluorescent property or a spectral property of the target cell. The method also includes measuring at least one property of the hydrogel particle using the cytometric device.

An apparatus and method for generating a controlled, predictable, reproducible and variable-size distribution of particulate matter (PM), particle number (PN) and/or facsimile/simulation, derived from vaporizing and condensing a specialized liquid, utilizing a vapor delivery device; a filter capability so as to remove a significant amount of ambient PM/PN as a secondary calibration process for the identification of fine and ultra-fine particles (e.g., 0.3 micrometers and smaller) as well as a computer-controlled ability to perform a pre-determined series of calibration routines, housed in a container.

A method of selecting a type of particle for use in standardisation and/or calibration of a flow cytometer. The method includes determining the location of two or more particle focal points of particles flowing through a cross section of a channel in the flow cytometer; for each type of particle, determining for each particle focal point, for a beam of light directed at a type of particle at said particle focal point from a first direction, the total intensity of light scattered along a second direction; determining the difference between the highest and lowest determined light intensities of the light intensities determined at the two or more particle focal points; and selecting a type of particle for which the difference between the highest and lowest determined light intensities at the two or more particle focal points is below a predetermined threshold.

A set of 10 or more standards containing a defined number of particles from 1000 to 1,000,000 wherein the defined number of particles is within a degree of error of 10% or less between each standard of the set.

Determination of Analytes in a Sample Matrix by Solvent Extraction A method for the assay of one or more analytes in a sample matrix comprising the steps of: performing analyte extraction on the sample matrix, said analyte extraction comprising combining the sample matrix with a solvent for an extraction period which is less than that required for reaching equilibrium; and separating the analyte containing solvent from the sample matrix; next measuring a level of analyte present in the separated solvent; and then applying in a computer a calibration by which is established a mathematical relationship between levels of analyte extracted from each of a plurality of reference samples by means of the process employed above in the extraction for the sample matrix and a reference value of the levels of analyte for each reference sample to thereby derive a measure of the level of analyte in the sample matrix. Specifically a method to determine the amount of mycotoxins in cereal grain, especially OTA (ochratoxin A) and DON (deoxynivalenol) by mixing with a solvent comprising water alcohol mixture, with 20-40% ethanol by volume.