The invention encompasses a rapid and safe preparation method of sperm nuclei, improved sperm nuclei and a method of using the improved sperm nuclei to calibrate a flow cytometer with higher accuracy.

Hydrogel particles and their use in cytometric applications are described. The hydrogel particles described herein are selectively tunable to have at least one optical property substantially similar to at least one optical property of a target cell. In this regard, the hydrogel particles provided herein, in one aspect, are used as a calibration reagent for the detection of a target cell in a sample.

Various embodiments include an exemplary apparatus and method for insitu calibration of multiple flow-sensing devices within a dilution system. In one example, a calibration and dilution system includes a first mass-flow device to serve as a global reference, a second mass-flow device configured to be coupled to and provide a supply of clean gas to a primary diluter, and a third mass-flow device configured to be coupled to and provide a supply of clean gas to a secondary diluter, where the diluters are pneumatically coupled to one another through a gas-supply line. Multiple valves are coupled to at least the mass-flow devices and the diluters. The calibration and dilution system is arranged so that the mass-flow controllers can be calibrated in-situ without having to remove any of the mass-flow controllers from the calibration and dilution system. Other apparatuses, designs, and methods are disclosed.

Provided is a fine particle measuring device and the like at least including at least two light sources having different wavelength region, a detection unit that detects light from a fluorescent reference particle in accordance with excitation light from the light sources, and an information processing unit that compares, on the basis of information detected by the detection unit, a feature quantity of an output pulse based on a reference light source among the plurality of light sources with a feature quantity of an output pulse based on at least another light source among the plurality of light sources, and adjusts an output of the another light source.

An alarming method for a platelet aggregation sample can include providing a blood sample, preparing a first test sample from the blood sample under a first reaction condition, acquiring a test signal of the first test sample, and obtaining first platelet test data. The method can also include preparing a second test sample from the blood sample under a second reaction condition, acquiring a test signal of the second test sample, and obtaining second platelet test data. The method can further include obtaining an evaluation result based on the first platelet test data and the second platelet test data, determining whether the evaluation result meets a predetermined condition, and alarming that the blood sample may be the platelet aggregation sample if the evaluation result meets the predetermined condition. The second reaction condition may include a reaction condition for reducing the platelet aggregation degree of the blood sample.

Methods of calibration are provided. A method comprises introducing a material with cell-like properties and a known mass into a sensor on a measurement instrument to generate a calibration reading and adjusting an output module of the measurement instrument until the measurement instrument calibrates to the known mass for the material.

A method for determining a degree of impurity of water comprises performing (200) of a dynamic light scattering analysis of a multitude of samples of a water to be tested. Each sample of said multitude of samples comprises added single-size polymer beads of a respective size and in a respective known amount. A smallest size of the single-size polymer beads giving rise to a detectable signal, discernible over a background noise level, in a size distribution curve of the dynamic light scattering analysis is determined (220). A smallest amount of the single-size polymer of the determined smallest size giving rise to a detectable signal is determined (230). A degree of impurity of the water to be tested is assigned (240) in dependence of the determined smallest size and the determined smallest amount of the single-size polymer.

The invention encompasses a rapid and safe preparation method of sperm nuclei, improved sperm nuclei and a method of using the improved sperm nuclei to calibrate a flow cytometer with higher accuracy.

Provided are particle analyzers and related methods for verifying calibration status of the particle analyzer, including independently of the presence or absence of particles. The method and analyzers include use of distinct and non-interfering time frequency domains: a middle frequency time domain and a low frequency time domain, and optionally a high frequency time domain. The high frequency time domain generates a laser facet drive current frequency modulation to prevent the laser facet from spatial-mode hopping. The middle frequency time domain is for particle detection. The low frequency time domain is for calibration status, including laser-pulse-light self-diagnostics, for the health or calibration status of the analyzer. By carefully selecting the frequency time domain ranges, there is non-interference, with the ability to self-diagnose the instrument that is particle-independent.

Disclosed are verification techniques that can be implemented by a device that conducts biological tests to verify that the specimen holding area carries a valid biological specimen. In certain embodiments, the testing device includes a receiving mechanism to receive a carrier, and the carrier includes a holding area that is to carry or to be exposed to a biological specimen. The device can also include a camera module arranged to capture imagery of the carrier, and a processor. In some examples, the processor can capture the imagery of the carrier and identify a visual cue on the carrier. Then, the processor can verify, based on a manner of how the visual cue is displayed in the captured imagery, whether the holding area carries a valid biological specimen.