Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples.

An image sensor package, a system, and a method for counting fine particles by using a virtual grid line are provided. The image sensor package includes an image sensor array, a grid pattern layer formed on an outer area of the image sensor array and including a plurality of protruding patterns spaced apart from each other while protruding toward the central area of the image sensor array to form a virtual grid line, a dam pattern layer formed on the grid pattern layer, having a specific height, and configured to form a channel or a chamber for receiving the fine particles to be counted, and a cover glass formed on the dam pattern layer.

A particle sizing method which allows for counting and sizing of particles within a colloidal suspension flowing through a single-particle optical sizing sensor SPOS apparatus using pulse height detection and utilizing non-parallel and non-uniform illumination within the sensing region of the flow cell. The method involves utilizing a deconvolution process which requires the SPOS apparatus to be characterized during a calibration phase. Once the SPOS apparatus has been characterized, the process of deconvolution after a data collection run, recursively eliminates the expected statistical contribution to the pulse height distribution PHD histogram in all the lower channels from the highest channel height detected, and repeating this for all remaining channels in the PHD, removing the contributions from largest to smallest sizes.

Aspects of the present disclosure include methods for determining baseline noise of a photodetector (e.g., in a light detection system of a particle analyzer). Methods according to certain embodiments include irradiating a sample having particles in a flow stream, detecting light with the photodetector from the irradiated flow stream, generating data signals from the detected light and calculating a moving average mean squared error of the generated data signals to determine the baseline of the photodetector. Systems (e.g., particle analyzers) having a light source and a light detection system that includes a photodetector for practicing the subject methods are also described. Integrated circuits and non-transitory computer readable storage medium are also provided.

Systems and methods are provided for identification and discrimination of subpopulations within a mixture of particles. The systems and methods implement continuous calibration of the classification of particles within the mixture to provide consistency in operation and to reduce inter- and intra-batch processing variation. The systems and methods produce advantageously sorted particle products.

Embodiments include a device for testing biological specimen. The device can include a receiving mechanism to receive a carrier. The carrier may include a holding area. The device includes a camera to capture a plurality of images of the holding area. A processor in the device may utilize the camera module to: adaptively select, based on the plurality of images of the holding area, an analytical algorithm suitable for a motion property that is characteristic of the biological specimen being tested; and perform a set of analytic processes that corresponds to the selected analytic algorithm on the captured imagery to generate an analytic result associated with the biological specimen.

The subject invention pertains to compositions of novel analogs of red blood cells that are distinguishable from white blood cells in a hematological instrument and processes for manufacturing such analogs. The processes for creating the compositions comprise washing, shrinking, and fixing cells at temperatures at or below room temperature.

A system and method for verifying a mechanical system chip detection capability, the method including determining a slurry injection location in machinery according to a targeted obstruction in the machinery in relation to a target chip detector of the machinery, placing an injection tube through a machinery casing of the machinery, providing a slurry tube connected to the injection tube, the slurry tube containing a slurry comprising conductive chips and a lubricant, operating the machinery until the machinery meets one or more predetermined operating parameters, injecting the slurry into the machinery casing during operation of the machinery and after the machinery meets the one or more predetermined operating parameters, and monitoring the target chip detector for detection of the conductive chips during the operation of the machinery.

Aspects of the present disclosure include methods for determining a parameter of a photodetector (e.g., a photodetector in a particle analyzer). Methods according to certain embodiments include irradiating a photodetector positioned in a particle analyzer with a light source (e.g., a continuous wave light source) at a first intensity for a first predetermined time interval, irradiating the photodetector with the light source at a second intensity for a second predetermined time interval, integrating data signals from the photodetector over a period of time that includes the first predetermined interval and the second predetermined interval and determining one or more parameters of the photodetector based on the integrated data signals. Systems (e.g., particle analyzers) having light source and a photodetector for practicing the subject methods are also described. Non-transitory computer readable storage medium having instructions stored thereon for determining a parameter of a photodetector according to the subject methods are also provided.

To provide a technique capable of handling quantitative data in a spectrum type microparticle measurement device without causing deterioration of the SNR. The present technology provides a microparticle measurement spectrometer including: a spectroscopic element that disperses light emitted from microparticles flowing through a flow path; and a photoelectric conversion array that has a plurality of light receiving elements having different detection wavelength ranges and converts optical information obtained by the light receiving elements into electrical information, in which the photoelectric conversion array has a uniform output of all channels when light with which the amount of light per unit wavelength becomes same is incident.