The subject matter described herein relates to devices and methods for capturing micrometer scale objects from a fluid flow, and more particularly, to devices and methods for capturing cells. The devices generally comprise an array of posts configured and arranged to selectively direct the micrometer scale objects toward trapping channels defined between posts in which the micrometer scale objects can be trapped, and to direct smaller particles through bypass channels around the posts. Methods for using the devices to trap cells, analyze cells and treat cancer are also described.

Embodiments of the present disclosure can include a method comprising: providing a plurality of cells to a microchannel, the microchannel coated in at least one cell adhesion entity and comprising a compressive surface and a first outlet, the compressive surface defining a compression gap, flowing the plurality of cells through the microchannel, wherein the flowing comprises: compressing the plurality of cells underneath the compressive surface; and exposing the plurality of cells to the at least one cell adhesion entity, wherein the exposing causes a first portion of the cells having a first adhesion property to temporarily bind to the cell adhesion entity; and collecting the first portion of cells at the first outlet; wherein the compression gap has a height of from 75% to 95% an average diameter of the plurality of cells.

A microfluidic chip configured to move a microdroplet along a predetermined path, includes a plurality of probe electrode groups spaced apart along the predetermined path. Each of the plurality of probe electrode groups includes a first probe electrode and a second probe electrode spaced apart from each other. The first probe electrode and the second probe electrode among a plurality of first probe electrodes and a plurality of second probe electrodes are configured to form an electrical loop with the microdroplet to thereby facilitate determining a position of the microdroplet.

A microfluidic device comprises at least one inlet for receiving a sample comprising target cells and non-target cells; a first spiral channel portion having an upstream end in a central region and a downstream end in a peripheral region, the upstream end being coupled to the inlet, the first spiral channel portion being configured such that the target cells and the non-target cells occupy different streams at the downstream end; a first waste outlet arranged to couple with streams of non-target cells at the downstream end of the first spiral channel portion; a link channel portion arranged to couple with streams of target cells at the downstream end of the first spiral channel portion; a second spiral channel portion having an upstream end in a peripheral region and a downstream end in a central region, the upstream end of the second channel portion being coupled to the link channel portion, the second spiral channel portion being configured such that the target cells and the non-target cells occupy different streams at the downstream end; a second waste outlet arranged to couple with streams of non-target cells at the downstream end of the second spiral channel portion; and a sample outlet arranged to couple with streams of target cells at the downstream end of the second spiral channel portion.

Methods of forming a shear-mode chemical/physical sensor for liquid environment sensing on V-shaped grooves of a [100] crystal orientation Si layer and the resulting devices are provided. Embodiments include forming a set of V-shaped grooves in a [100] Si layer over a substrate; forming an acoustic resonator over and along the V-shaped grooves, the acoustic resonator including a first metal layer, a thin-film piezoelectric layer, and a second metal layer in an IDT pattern or a sheet; and forming at least one functional layer along a slope of the acoustic resonator.

An exemplary method and system is disclosed that facilitate the integration of multiplexed single-cell impedance cytometry in a high throughput format, which can be deployed upstream from microfluidic sample preparation and/or downstream to microfluidic cell separation. In exemplary method and system may employ impedance-based quantification of cell electrophysiology on the same microfluidic chip (i.e., on-chip) to provide distinguishing phenotypic information on the sample, without the need for additional sample handling, preparation or dilution steps as would be needed for other flow cytometry techniques.

The disclosure presented herein provides methods for quantifying viable cells and particulate cell impurities in a cell-based product sample. The method is implemented on a convolutional neural network (CNN) that learns to classify flow-imaging microscopy (FIM) images. The CNN learning is accomplished by using a training set of classified images of viable cells and different types of impurities.

An electronic-sensing & magnetic-modulation (ESMM) biosensor device and methods of using the same, where the device incorporates electrical, microfluidic, and magnetic subsystems. The device and methods of using such a device can be applied to high throughput point-of-care screening for the quantification and evaluation of a subject's immune response to pathogenic infections, which can be useful for: early diagnosis, stratifying high-risk subjects infected with a pathogen; and determining the status or effectiveness of a therapeutic response.

A microfluidic diagnostic chip may comprise a main fluid channel comprising a main pump, a secondary fluid channel branching off from the main fluid channel, and a secondary pump within the secondary fluid channel wherein the secondary pump is to pull a particle of analyte of a first size from a fluid passing through the main channel, the fluid comprising particles of analyte of the first size and of a number of larger sizes. A method of analyzing an analyte on a microfluidic chip may comprise pumping, with a main microfluidic pump, a fluid comprising an analyte particle through a main microfluidic channel fluidly coupled to a fluid slot and sorting the analyte particle within the fluid through a secondary microfluidic channel by pulling the analyte particle into the secondary microfluidic channel with a secondary microfluidic pump.

A system and method for isolating and analyzing single cells, comprising: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.