Disclosed herein are imaging-based devices and systems for measuring sperm motility in samples of human or animal origin. The disclosed devices and systems have particular applicability in the fields of agricultural and clinical diagnostics, as well as in vitro fertilization.

The present invention provides a magnetophorisis measuring system, comprising a microscope device, a magnetic field generator, an image capturing unit, and a processing unit. The microscope device is utilized to magnify a sample liquid having a plurality of objects respectively having a plurality of magnetic particles. The magnetic field generator is utilized to provide an external magnetic field on the sample liquid such that the objects are moved by the external magnetic field. The image capturing unit is utilized to capture a dynamic image with respect to the fluid sample in a view field of the microscope device. The processing unit receives the dynamic image, automatically detects and locks moving objects, determines a motion status corresponding to each object, and quantifies the magnetic particles according to motion status of each object.

A method of foreign object debris discrimination includes illuminating a particle located within a sensing volume with a modulated electromagnetic radiation pulse emitted from a source; receiving one or more electromagnetic radiation return signals that have been scattered by the particle illuminated by the modulated electromagnetic radiation pulse at a detector; mixing, using a controller, the electromagnetic radiation return signal of amplitude I.sub.RS and frequency f.sub.RS with a reference signal of amplitude I.sub.LS and frequency f.sub.RS; analyzing, using the controller, an amplitude of the mixed signal ?{square root over (I.sub.LSI.sub.RS)}, and frequency of the mixed signal, f.sub.RS?f.sub.LS; and classifying, using the controller, a particle position, a velocity, and electromagnetic characteristic of the particle based on the amplitude, ?{square root over (I.sub.LSI.sub.RS)}, and frequency, f.sub.RS?f.sub.LS of the mixed signal.

A specimen processing system includes a plate for supporting a specimen system, wherein the specimen system includes a container and a specimen contained therein. The specimen processing system further includes a camera disposed above the plate and configured to generate images of the specimen system, a light source disposed beneath the plate for radiating light towards the plate, a light stop for blocking a portion of the light from reaching the specimen system to produce darkfield illumination of the specimen at the camera, and one or more processors electronically coupled to the camera and configured to track a position of the specimen within the specimen container during a specimen processing protocol based on the images.

The present technology relates to systems and associated methods for measuring properties of particles in a solution. In one or more embodiments, a particle measurement system is configured to generate a reference signal, communicate the reference signal across a plurality of resistors and overlapping pairs of electrodes that define detection regions for particulates traveling through a microchannel, and measure various properties of the particles based on detecting changes in the communicated reference signal.

Method for observing an emission of fluorescence light or luminescence light from a moving particle in a sample, in a spectral emission band, the method comprising: a) forming a detection image of the sample in a spectral detection band, the spectral detection band being different from the spectral emission band; b) forming an emission image of the sample in the spectral emission band;
the method being such that: steps a) and b) are reiterated; the method comprising: c) on the basis of each detection image resulting from step a) of each iteration of steps a) and b), detecting the particle and determining a region of interest around the particle; d) on the basis of the region of interest resulting from each step c), extracting a region of interest from each emission image of the sample.

Method and microfluidic system (1) for the manipulation of particles; a detection device (7) acquires images of a specific particle (5) in a first position (IP) and in a second position (IIP); the difference is made between the two images in order to obtain a derived image in which the contours and the morphological characteristics of the specific particle (5) are more evident; in this manner, the type and the position of the particles can be identified more clearly, continuously and in a time-saving manner.

A microfluidic apparatus includes a microfluidic chip for MicroOrganoSpheres (MOS) generation. A first channel is defined in a surface of the microfluidic chip and includes: a droplet generation portion including an inlet portion, a junction between the inlet portion and an emulsifying fluid channel, and a chamber downstream of the junction. A cross-sectional area of the chamber is larger than that of the inlet portion. The first channel includes a polymerization portion downstream of the droplet generation portion, the polymerization portion having a serpentine configuration. The apparatus includes a cartridge for MOS demulsification, including: a collection container; a substrate disposed on the collection container, and a membrane disposed between the collection container and the surface of the substrate. A second channel is defined in the surface of the substrate that faces the collection container and is fluidically connected to an output of the polymerization portion of the first channel.

A microparticle detection system includes a stage unit including a mounting surface on which a fluid device having a flow path through which a sample containing microparticles is movable is capable of being mounted, an emission unit configured to emit illumination light to the flow path, an imaging unit configured to image scattered light generated from microparticles in the sample when illumination light is emitted, an identification unit configured to identify the microparticles included in the image for each of the microparticles on the basis of the image captured by the imaging unit, a particle size determination unit configured to determine a particle size of the microparticle for each of the microparticles identified by the identification unit, a zeta potential determination unit configured to determine a zeta potential of the microparticle for each of the microparticles identified by the identification unit, and a correlation unit configured to associate the particle size for each of the microparticles determined by the particle size determination unit with the zeta potential for each of the microparticles determined by the zeta potential determination unit for each of the microparticles.

The disclosure relates to microfluidic devices and methods of use thereof for monitoring the directionality, velocity, and migration persistence of neutrophils or other cells in the absence of chemical gradients for the purposes of detecting and quantifying abnormal neutrophil motility phenotypes, using low sample volumes and with minimal activation of the neutrophils. The devices and methods can be used to diagnose sepsis in subjects suspected of having sepsis or at risk of developing sepsis. The devices and methods can also be used to monitor the responses of patients to sepsis therapies.