Among other things, a first surface is configured to receive a sample and is to be used in a microscopy device. There is a second surface to be moved into a predefined position relative to the first surface to form a sample space that is between the first surface and the second surface and contains at least part of the sample. There is a mechanism configured to move the second surface from an initial position into the predefined position to form the sample space. When the sample is in place on the first surface, the motion of the second surface includes a trajectory that is not solely a linear motion of the second surface towards the first surface.

Among other things, a first surface is configured to receive a sample and is to be used in a microscopy device. There is a second surface to be moved into a predefined position relative to the first surface to form a sample space that is between the first surface and the second surface and contains at least part of the sample. There is a mechanism configured to move the second surface from an initial position into the predefined position to form the sample space. When the sample is in place on the first surface, the motion of the second surface includes a trajectory that is not solely a linear motion of the second surface towards the first surface.

Among other things, a first surface is configured to receive a sample and is to be used in a microscopy device. There is a second surface to be moved into a predefined position relative to the first surface to form a sample space that is between the first surface and the second surface and contains at least part of the sample. There is a mechanism configured to move the second surface from an initial position into the predefined position to form the sample space. When the sample is in place on the first surface, the motion of the second surface includes a trajectory that is not solely a linear motion of the second surface towards the first surface.

This technology relates in part to optical methods for analyzing particles, including nanoparticles, thereby determining their presence, identity, origin, size and/or number in a sample of interest.

Disclosed herein are methods for analyzing cell kinematics in a nucleated cell culture from a time-series sequence of multiple fluorescence microscopic images of the nucleated cell culture. The method includes the steps of, (a) identifying every cell nucleus in each fluorescence microscopic image; (b) identifying every cell cluster using the cell nuclei identified in the step (a); and (c) tracking the cells and/or cell clusters using the cell nuclei and cell clusters identified for the fluorescence microscopic images in steps (a) and (b) respectively.

The invention relates to a device and method for measuring the concentration, size and zeta potential of nanoparticles in liquids in scattered light mode and fluorescence mode, comprising the following features: a) a sample (28) is irradiated from above by a laser (1) via a beamsplitter (14) and an adjustable-focus liquid lens (9), and the reflected beam of light is deflected by the same beamsplitter (14) and a further liquid lens (8) onto a detector (5), and analysed; b) to observe the fluorescent light, a fluorescence filter (19) is connected in the convergent beam path between the liquid lens (8) and the detector (5), to increase the distance between the scattered light plane (31) and the fluorescence plane (30); and c) to control the measurement process, a particle tracking program, an optical control unit (15) and a display (2) with a touch screen are used.

A method of measuring a target substance contained in a sample may include passing a complex formed by allowing the target substance to react with a predetermined carrier through a pore provided in a substrate. A size of the complex may be measured based on change in electrical characteristics occurring when the complex is passed through the pore in the pass-through step. The number of molecules of target substance may be determined based on the size of the complex measured in the measuring step.

A simple sperm test kit 10 for performing a simple sperm test has a substrate 20 capable of being placed over a camera 30 of an information terminal 11, a recess 21 for containing semen A provided in the surface of the substrate 20, a cover 22 that covers the recess 21 for allowing external light to enter the recess 21, and a lens 23 provided within the substrate 20 on the lower side of the recess 21 for magnifying the semen A in the recess 21 and projecting an image on the back side of the substrate 20.

The present invention provides a number analyzing method, a number analyzing device, and a storage medium for number analysis, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device (8) in accordance with passage of subject particles through a through-hole (12). Also, a particle type distribution estimating program, which is a number deriving means, is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.

Disclosed are systems are methods for identifying the composition of single aerosol particles, particularly that of bioaerosol particles. A continuous timing laser tightly coupled with a pulse ionization laser is used to index aerosol particles, measure particle properties, and trigger the ionization laser to fire when each particle enters the beam of the trigger laser. Ionized fragments and optionally photons produced when each particle is struck by the ionization laser are analyzed using one or more detectors including a TOF-MS detector and an optical detector. Individual single particle spectra are aligned and denoised prior to averaging.