A combination of mutually exclusive cell-based analytical techniques can be applied to the same group of cells for analysis. The same group of cells can be prepared for analysis by each technique resulting with candidate cells targeted for mass cytometry analysis. This configuration allows for the correlation of the information between each technique to produce a matrix of multi dimension of cellular information with the same group of cells.

Methods, compositions and kits for determining the developmental potential of one or more embryos or pluripotent cells and/or the presence of chromosomal abnormalities in one or more embryos or pluripotent cells are provided. These methods, compositions and kits find use in identifying embryos and oocytes in vitro that are most useful in treating infertility in humans.

The present application discloses a method and apparatus for computing a nuclear reaction cross section and a path integral in particle transport, an electronic device, and a storage medium. The method includes: obtaining a product of a macroscopic nuclear reaction cross section of a material and an additional function by using a linear combination of basis functions; and obtaining a path integral of the product of the macroscopic nuclear reaction cross section of the material and the additional function by using the linear combination of basis functions. The additional function is a function of spatial position and energy, where the domain of the function is a set of points in space and a set of non-negative real number energy, and the range of the function is complex numbers or vectors of the complex numbers. Embodiments disclosed in the present application provide a supplementary computation method for a situation where an additional function appears or multi-group cross sections are used in computing a nuclear reaction cross section and a path integral in particle transport by using a linear combination of basis functions.

A method for determining a physical property related to a charge of a constituent of a sample, from field flow fractionation measurements with an additional electrical field, comprising the steps of obtaining a first fractogram of a first sample and a second fractogram of a second sample, wherein the first sample and the second sample are samples of a same substance, the first fractogram has been generated using a first electrical field, the second fractogram has been generated using a second electrical field, and a strength of the first electrical field and a strength of the second electrical field are different from each other; determining, by using a first mapping, from a first intensity value of the first fractogram, a first value and determining, by using a second mapping, from a second intensity value of the second fractogram, a second value; and determining, based on the first value and the second value, a physical property related to a charge of a constituent of at least one of the first sample and the second sample; wherein the first mapping maps the first intensity value to the first value of a first bijective function over time and the second mapping maps the second intensity value to the second value of a second bijective function over time.

A device for detecting at least one analyte, for example a cell, in a sample fluid, the device comprising a fluid channel having a longitudinal channel axis and structured to allow the sample fluid containing at least one analyte to pass through the fluid channel, at least one pair of electrodes, an electric field generating unit for generating an electric field between the at least one pair of electrodes, a detecting unit for detecting the at least one analyte in the sample fluid based on its passage through the electric field, wherein each electrode of the at least one pair of electrodes has a three-dimensionally structured electrode surface, which electrode surface is curved about the longitudinal channel axis. The device may further comprise an optic sensing unit structured to optically sense a sensing region of the fluid channel between the at least one pair of electrodes.

The present invention describes a fluidic device for measuring at least one of corpuscle mass density and weight. The fluidic device comprises a sedimentation chamber fluidly connected to an inlet channel configured to be immersed in a liquid. The fluidic device further comprises a pumping system connected to the sedimentation chamber. The pumping system is adapted to control the flow of liquid in the sedimentation chamber. A processor of the fluidic device is configured to obtain corpuscle data related to a corpuscle in at least one region of the sedimentation chamber; and calculate at least one of corpuscle mass density and weight based on the data received.

Various implementations, systems and methods are disclosed for continuous, near real-time, hands-off sampling of airborne particulate matter, and qualification and/or quantification of biomolecules in the sample representative for biologic or microbial contamination. The systems and methods may utilize an electrostatic precipitator for sampling the matter; and a measurement assembly configured to illuminate, excite, or breakdown the sampled matter by electromagnetic radiation, and to detect a spectrum, or one or more wavelength bands of the scatter emitted by the sample. In an exemplary implementation, a sputter deposition process is employed to configure the sample for an enhanced plasmon resonance. The measurement data may be transferred via wireless communication means for cloud storage and signal processing.

Disclosed is a method for predicting the gene transfer efficiency of animal cells, the method comprising: (1) measuring the cell complexity of transgenic animal cells; and (2) predicting the gene transfer efficiency based on values measured in step (1).

Aspects of the present disclosure include methods for assessing morphology of isolated cell nuclei (e.g., to determine viability of the cell nuclei) in a sample. Methods according to certain embodiments include measuring light from a sample having isolated cell nuclei in a flow stream, generating an image of the cell nuclei from the measured light and assessing morphology of the cell nuclei based on the generated images of the cell nuclei. In some embodiments, sorting gates are determined based on images or image parameters calculated for the cell nuclei in the sample. Systems and integrated circuit devices (e.g., a field programmable gate array) for practicing the subject methods are also described. Non-transitory computer readable storage medium are also provided.

The present technique provides a simple, cost-effective and robust method for re-alignment of interfaces between cell conjugates parallel to the coverslip. The technique is based on placement of two cell types, to subsequently form an interaction between the cell types, to opposing coverslips and then bringing them together before or during imaging, for either fixed or live cell imaging. Spacer particles having defined parameters control the z-separation and the relative lateral position of the opposing coverslips. We show that our method allows most types of super-resolution imaging.