Aiming to provide a method capable of reciprocatingly delivering various liquids without bringing air into a microchannel, a detection system and a detection apparatus for implementation of this method. In order to achieve at least one of the above aims, provided is a method of inserting a pipette tip into a liquid injection portion of a detection chip including: the microchannel; the liquid injection portion connected to one end of the microchannel; and a reservoir connected to the other end of the microchannel, injecting and aspirating the liquid by the pipette tip, and reciprocatingly delivering the liquid into the microchannel. At this time, the following steps are executed in this order, the steps including: inserting the pipette tip into the liquid injection portion up to a position at which an end of the pipette tip comes below a liquid level when the liquid is injected into the liquid injection portion; injecting the liquid from the pipette tip into the liquid injection portion; generating a negative pressure in the liquid injection portion to raise the level of the liquid in the liquid injection portion; and performing either aspiration of the liquid in the liquid injection portion by the pipette tip, or injection of the liquid into the liquid injection portion by the pipette tip and aspiration of the liquid inside the liquid injection portion.

Aiming to provide a method capable of reciprocatingly delivering various liquids without bringing air into a microchannel, a detection system and a detection apparatus for implementation of this method. In order to achieve at least one of the above aims, provided is a method of inserting a pipette tip into a liquid injection portion of a detection chip including: the microchannel; the liquid injection portion connected to one end of the microchannel; and a reservoir connected to the other end of the microchannel, injecting and aspirating the liquid by the pipette tip, and reciprocatingly delivering the liquid into the microchannel. At this time, the following steps are executed in this order, the steps including: inserting the pipette tip into the liquid injection portion up to a position at which an end of the pipette tip comes below a liquid level when the liquid is injected into the liquid injection portion; injecting the liquid from the pipette tip into the liquid injection portion; generating a negative pressure in the liquid injection portion to raise the level of the liquid in the liquid injection portion; and performing either aspiration of the liquid in the liquid injection portion by the pipette tip, or injection of the liquid into the liquid injection portion by the pipette tip and aspiration of the liquid inside the liquid injection portion.

The present disclosure provides a sample test card and a sample loading method thereof, relates to the technical field of microbiological testing. The present disclosure includes sample wells arranged in an array; the sample wells are connected together through a flow channel network, and the sample wells are filled with a sample through a unified intake port. Sample filling is completed by vacuuming; during filling, a liquid sample is firstly filled, and air or other inert gas or insoluble liquid is filled; liquid sample volume and air volume are formed in proportion in the sample wells. In the present disclosure, the filled liquid sample is controlled not to fill the sample wells completely, and there are sufficient air space in sample wells, so that the sample wells are independent from each other to avoid contamination, and more sample wells can be arranged on the test card with the same size.

An embodiment provides a method for cleaning a sample cell of a turbidimeter, including: introducing an apparatus comprising a cleaning component into the sample cell, wherein upon introduction of the apparatus into the sample cell a secondary flow path out of the sample cell is opened; engaging a functionality of the apparatus to clean the sample cell; and removing a plurality of particles from the sample cell via the secondary flow path. Other aspects are described and claimed.

The present invention relates to an automated liquid transmission cell system capable of transferring solvents and samples to clean and fill the transmission cells. Three valves allow a plurality of system configurations to perform a variety of flushing and filling processes by changing the position of each valve. A plurality of tubes are connected to two of the valves such that changing the valve positions will redirect fluid transmission paths.

The present invention relates to an automated liquid transmission cell system capable of transferring solvents and samples to clean and fill the transmission cells. Three valves allow a plurality of system configurations to perform a variety of flushing and filling processes by changing the position of each valve. A plurality of tubes are connected to two of the valves such that changing the valve positions will redirect fluid transmission paths.

An apparatus (150) comprises a first detection chamber (130) for receiving microorganisms and configured to allow detection of the microorganisms via detection of scattered light from the first detection chamber (130); a medium (120) configured to permit passage of microorganisms from a sample (110) through the medium (120) into the first detection chamber (130); and at least one second detection chamber (140) configured to allow detection of the microorganisms via detection of scattered light from the at least one second detection chamber (140).

A highly-reliable absorbance measuring device that enables highly-accurate measurement of absorbance, and a method thereof are provided. A liquid containing unit that can contain a chemical substance solution to be measured, a nozzle that communicates with a suction/discharge mechanism that sucks/discharges gas, a flow tube that includes a mouth part, which can be inserted into the liquid containing unit, at a lower end and that is detachably attached to the nozzle at an upper end, an emitting end that can emit measurement light, a light receiving end that can receive the light emitted from the emitting end, and a control unit are included. The control unit is configured to suck a prescribed amount of the chemical substance solution into the flow tube, and to lead absorbance on the basis of intensity of transmitted light acquired by emission of measurement light in a vertical direction into the flow tube.

A highly-reliable absorbance measuring device that enables highly-accurate measurement of absorbance, and a method thereof are provided. A liquid containing unit that can contain a chemical substance solution to be measured, a nozzle that communicates with a suction/discharge mechanism that sucks/discharges gas, a flow tube that includes a mouth part, which can be inserted into the liquid containing unit, at a lower end and that is detachably attached to the nozzle at an upper end, an emitting end that can emit measurement light, a light receiving end that can receive the light emitted from the emitting end, and a control unit are included. The control unit is configured to suck a prescribed amount of the chemical substance solution into the flow tube, and to lead absorbance on the basis of intensity of transmitted light acquired by emission of measurement light in a vertical direction into the flow tube.

A liquid transfer monitoring method to monitor a transfer state of a liquid such as a sample or a reagent having a high transparency which transmits light is provided. The liquid transfer monitoring method comprises a step of transferring a liquid 30 held in a first liquid holding portion 21 to a second liquid holding portion 22 connected to the first liquid holding portion 21, a step of acquiring information 40 relating to a scattered light intensity obtained by irradiating light 91 on the irregular inner surface 23 of at least one of the first liquid holding portion 21 and the second liquid holding portion 22 after starting the transfer of the liquid 30, and a step of monitoring the liquid transfer to the second liquid holding portion 22 based on the information 40 relating to the intensity of scattered light.