A sample observation device includes an irradiation unit that irradiates a sample with planar light, a scanning unit that scans the sample in one direction with respect to an irradiation surface of the planar light, an image formation unit that forms images of fluorescent light and scattered light from the sample, an imaging unit that outputs first image data based on a light image of the fluorescent light and second image data based on a light image of the scattered light, an image processing unit that generates a fluorescent light image on the basis of a plurality of pieces of first image data and generates a scattered light image on the basis of a plurality of pieces of second image data, and an analysis unit that specifies an area in which there is the sample in the fluorescent light image on the basis of the scattered light image, and sets an analysis area in the fluorescent light image on the basis of the area in which there is the sample.

Techniques are disclosed relating to fluorescence-based flow cytometry. A flow cytometer may include a partially-reflective surface configured to reflect a first portion of fluorescent emissions from a sample to a first optical sensor and direct a second, greater portion of fluorescent emissions from the sample to a second optical sensor and a controller configured to determine a value representing the intensity of the fluorescent emissions based on a first measurement taken by the first optical sensor, a second measurement taken by the second optical sensor, or both. A flow cytometer may include a baseplate with a first side and a second, opposing side with a flow cell, a laser, and a reflective surface disposed above the first side and an optical sensor and isolating material disposed below the second side. The reflective surface receives fluorescent emissions and reflects at least a portion through the baseplate to the optical sensor. A flow cytometer may include a flow cell, a laser, a first optical sensor positioned to measure scattered laser light, a second optical sensor positioned to measure fluorescent emissions, and a controller configured to adjust the measurements taken by the second optical sensor based on a comparison of measurements taken by the first optical sensor with expected measurements based on a known beam profile of the laser beam.

The present invention relates to the design, construction, and operation of a laser air-sampling multi-spectrometer; its operation with variable laser energy to simultaneously and/or sequentially perform spectrometric techniques of LAS, LEFS, RSS, and LIBS. The combined spectrometric operation will detect gas and particulate chemicals directly in a flowing stream of air sample and/or particulate chemicals on filter collected from the flowing stream of air sample.

Described herein is a sensor for a virtually simultaneous measurement of transmission and/or forward scattering and/or remission and for a simultaneous measurement of the transmission and forward scattering or the transmission and remission of a liquid sample. Further described herein is a method for a virtually simultaneous measurement of transmission and/or forward scattering and/or remission and for a simultaneous measurement of the transmission and forward scattering or the transmission and remission of a liquid sample using a sensor according to the invention. Further described herein is a method for using the sensor according to the invention in order to determine the color properties of painting agents such as lacquers, dyes, pastes, and pigments or dilutions thereof.

The invention relates to a device (1) and a corresponding method for mid-infrared microscopy and/or analysis, the device (1) comprising at least one radiation unit (10) configured to generate radiation (11) of time-varying intensity, the radiation (11) comprising one or more wavelengths in the mid-infrared spectral range, at least one refractive and/or reflective optical unit (12) which is configured to focus and/or direct the radiation (11) to at least one region or point of interest (20) located on and/or within an object (2), at least one detection unit (18) configured to detect ultrasound waves (17) emitted by the object (2) at the at least one region or point of interest (20) in response to an interaction of the radiation (11) with the object (2) and to generate according detection signals, and an evaluation unit (25) configured to derive information regarding at least one property of the object (2) from the detection signals and/or to generate a spatial and/or spatio-temporal distribution of the detection signals or of information derived from the detection signals obtained for the at least one region or point of interest (20) located on and/or within the object (2).

Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples. Specifically, methods are provided for detecting and measuring, in a sample, cell morphology; measurement of cell numbers; detection of particles; measurement of particle numbers; and other properties and quantities of or in a sample. Some embodiments may use a sample holder comprising a sample chamber configured to hold said sample, at least a portion of said sample holder comprising an optically transmissive material, said optically transmissive material comprising an optically transmissive surface and a reflective surface.

Techniques are disclosed relating to fluorescence-based flow cytometry. A flow cytometer may include a partially-reflective surface configured to reflect a first portion of fluorescent emissions from a sample to a first optical sensor and direct a second, greater portion of fluorescent emissions from the sample to a second optical sensor and a controller configured to determine a value representing the intensity of the fluorescent emissions based on a first measurement taken by the first optical sensor, a second measurement taken by the second optical sensor, or both. A flow cytometer may include a baseplate with a first side and a second, opposing side with a flow cell, a laser, and a reflective surface disposed above the first side and an optical sensor and isolating material disposed below the second side. The reflective surface receives fluorescent emissions and reflects at least a portion through the baseplate to the optical sensor. A flow cytometer may include a flow cell, a laser, a first optical sensor positioned to measure scattered laser light, a second optical sensor positioned to measure fluorescent emissions, and a controller configured to adjust the measurements taken by the second optical sensor based on a comparison of measurements taken by the first optical sensor with expected measurements based on a known beam profile of the laser beam.

A biosensing system includes a biosensor, a light source, first and second photodetectors, and a calculator. The light source is disposed to irradiate the biosensor, so as to generate two or more of a coupled light beam, a reflected light beam, a transmitted light beam and a diffracted light beam. The first photodetector is disposed to measure an intensity of one of the generated light beams that is indicative of an effect of an analyte on light to obtain a first intensity value. The second photodetector is disposed to measure an intensity of another one of the generated light beams that is indicative of an effect of the analyte on light to obtain a second intensity value. The calculator performs compensation calculation based at least on the first and second intensity values.

An agricultural planter having sensors for measuring multiple soil properties adjusts planting depth and seeding rate in real time based on the measured soil properties. An optical module is carried by the planter for collecting soil reflectance data. A pair of soil contact blades protrude from or are embedded in the optical module for collecting soil EC data and soil moisture data. A switching circuit or phase lock loop allows the same soil contact blades to feed signals to both a soil EC signal conditioning circuit and a soil moisture signal conditioning circuit. The soil moisture data can be used to calibrate the soil EC data and the soil reflectance data to compensate for effects of changing soil moisture conditions across a field. The sensor module can be positioned behind a seed tube and used as a seed firmer, or incorporated into a seed tube guard.

The present invention discloses a multi-mode imaging optical system. The multi-mode imaging optical system includes a stage configured to hold a to-be-tested sample. An imaging unit, implements in-situ imaging of the to-be-tested sample. An absorption and forward scattering illumination unit, irradiates the to-be-tested sample, and forms absorption imaging or forward scattered light imaging in the imaging unit. A side scattering illumination unit, performs a first oblique illumination on the to-be-tested sample, so that scattered light of microparticles in the to-be-tested sample forms side scattered light imaging in the imaging unit. A fluorescent illumination unit, performs a second oblique illumination on the to-be-tested sample, and excites the microparticles in the to-be-tested sample to emit fluorescence, where the fluorescence forms fluorescence imaging in the imaging unit.