A change in a substance in a depth direction of an analyte is easily estimated. An analysis and observation device includes: a library holding section that holds a substance library in which a substance is associated with a type of an element constituting the substance and a content of the element; and a component analysis section that estimates a type of an element constituting a substance and a content of the element based on a spectrum, and estimates the substance based on estimated characteristics and the substance library. The component analysis section estimates a type of an element constituting a substance, a content of the element, and the substance at each of a plurality of positions having different analysis depths.

An identification apparatus includes a window unit including a passage surface on an upper side configured to allow a sample supplied from a conveyance unit to slide along and pass on the passage surface, a light irradiation unit disposed below the window unit, spaced a certain distance from the passage surface, and configured to irradiate the sample with a primary light through the window unit, a light collection unit disposed below the window unit and configured to collect a secondary light from the sample through the window unit, and an acquisition unit configured to acquire identification information for identifying a property of the sample based on the secondary light collected by the light collection unit.

Disclosed is a method for diagnosing a neurodegenerative disease in a subject. The method comprises obtaining from the subject a sample comprising at least one live blood cell, and optionally isolating at least one live blood cell from the sample. The method further comprises generating one or more multispectral or hyperspectral images of the at least one cell, and analysing spectral characteristics of autofluorescence from the at least one cell. Also disclosed is a system configured to aid in the detection or diagnosis of a neurodegenerative disease. Also disclosed is a method for selecting a subject for treatment for a neurodegenerative disease. Also disclosed is a method for monitoring the response of a subject to a therapeutic treatment for a neurodegenerative disease. Also disclosed is a protocol for monitoring the efficacy of a therapeutic treatment for a neurodegenerative disease.

The present invention relates to a passive terahertz biometric imaging device configured to be arranged under an at least partially transparent display panel and configured to capture a terahertz image of an object located on an opposite side of the transparent display panel, the terahertz biometric imaging device comprising: an image sensor comprising an antenna pixel array arranged to detect terahertz radiation produced by the object, for capturing a terahertz image of the object.

A method for optical imaging of single protein molecules including tethering single protein molecules via a flexible polymer linker to a glass slide having a surface coated with an indium tin oxide (ITO) so that the single protein molecules are tethered to the coated surface. The single protein molecules are driven into oscillation by applying an alternating electric field to the coated surface and the glass slide is located in the field of view of an objective lens. Incident light is directed onto the coated surface from an angle to generate an evanescent field and produce scattered light. The scattered light is collected and imaged by a CMOS imager to record a sequence of images of the scattered light. A Fast Fourier Transform (FFT) filter is applied to each pixel of the recorded image sequence to produce an oscillation amplitude image from which size, charge, and mobility of the plurality of single protein molecules can be determined.

An imaging target includes a light emitter configured to emit visible light from a surface of the imaging target, and an imaging device captures an image of at least the light emitter of the imaging target and acquires image data including the light emitter.

Methods and systems for physical expansion and imaging of biological samples are described herein. In one aspect of the disclosure, a method for preparing a biological sample for the purpose of generating images of its ultrastructure with an imaging instrument includes a) physically expanding the sample by at least a factor of two in at least one dimension; and b) bulk labeling a plurality of components of the sample with at least one reagent to introduce contrast.

An inspection apparatus including an illumination light source, a sensing probe and a processing device is provided. The illumination light source emits an illumination beam to simultaneously irradiate the plurality of light-emitting diode. The sensing probe is configured to measure a charge distribution, an electric field distribution, or a voltage distribution on the plurality of light-emitting diodes simultaneously irradiated by the illumination beam. The processing device determines a plurality of electro-optical characteristics of the plurality of light-emitting diodes through the charge distribution, the electric field distribution, or the voltage distribution on the plurality of light-emitting diodes simultaneously irradiated by the illumination beam. Moreover, a method of for inspecting light-emitting diodes is also provided.

Systems for and methods for detecting samples and other compounds of interest are disclosed herein. The system can include an illumination source that is configured to emit light in a variety of different wavelength bands, an optical filter, a camera chip, and an optical path configured to direct the light emitted by the first illumination source at a target and direct the light reflected from the target to the at least one camera chip. The illumination source and the optical filter are transitionable between a first tuning state corresponding to a target tissue and a second tuning state corresponding to a background tissue. The system can be configured to record images at the different tuning states and generate a score image corresponding to the target tissue based on the recorded images.

A high-throughput optofluidic device for detecting fluorescent droplets is disclosed. The device uses time-domain encoded optofluidics to detect a high rate of droplets passing through parallel microfluidic channels. A light source modulated with a minimally correlating maximum length sequences is used to illuminate the droplets as they pass through the microfluidic device. By correlating the resulting signal with the expected pattern, each pattern formed by passing droplets can be resolved to identify individual droplets.