A device for automated characterization of radioactive analytes that provides an integrated system with liquid handling and plate reading components. The device can be further configured to include a chromatographic subsystem. Also provided is a method of using such a device, providing addition of a radioactive sample and a sequence of operations involving the abovementioned components of the system. The system is configured with radiation shielding in such a way that manipulations of radioactive samples do not interfere with concurrent radioactive measurements.

A method of quantifying ammonia, the method includes performing a first reaction in which a test liquid containing ammonia is reacted with adenosine triphosphate and L-glutamic acid in the presence of glutamine synthetase to produce phosphoric acid, performing a second reaction in which the produced phosphoric acid is reacted with pyruvic acid in the presence of pyruvate oxidase, and measuring a component consumed or produced by the second reaction, to quantify ammonia, wherein a reaction to produce adenosine triphosphate from adenosine diphosphate mediated by pyruvate kinase is not carried out.

Disclosed is a test strip device, comprising a strip body and a blocking element. The strip body has a first face and a second face. The strip body has an injection opening, a flow channel and a reaction receptacle. The injection opening reaches the first face, the flow channel is in fluid communication between the injection opening and the reaction receptacle, and the flow channel is in fluid communication with the injection opening through a flow channel opening. The blocking element is vertically movably disposed in the injection opening, and the blocking element selectively closes the flow channel opening. Therefore, the back flow of a sample can be prevented, so as to ensure chemical reaction of the sample and a reagent and thus improve accuracy of the test results.

Paper microfluidic devices for the detection of bodily fluids are provided. Such devices can be used, for example, for detection of bodily fluids from or at crime scenes, including blood, saliva, semen, urine, feces, vaginal fluids, and perspiration. Detection can be performed using colorimetric reagents that react when placed in contact with the fluid of interest. A single device can be used to test for multiple bodily fluids at the same time.

A device is disclosed for conducting a non-invasive analysis of a bodily fluid to determine the presence and level of a certain constituent carried by the bodily fluid. An indicator formulation of the device changes color in response to exposure to the constituent to provide a visible indication of the presence and level of the constituent carried by the bodily fluid. A carrier substrate of the device is constructed of a material having voids providing a high void volume within the substrate. The device is made by applying a chromagen to the carrier substrate to create a chromagen-laden carrier member. Then, a selected reagent having a particular constituent-specific formulation is applied to the chromagen-laden member. The selected reagent then combines with the chromagen thereby establishing the indicator formulation within the carrier substrate in place for reception of a sample of the bodily fluid.

The invention relates to methods for detecting target DNA sequences, particularly for detecting Leishmania infection, comprising a recombinase-polymerase isothermal amplification and detection by electrochemistry. The invention also relates to kits and oligonucleotides for performing said methods.

Disclosed is a test strip device, comprising a strip body and a blocking element. The strip body has a first face and a second face. The strip body has an injection opening, a flow channel and a reaction receptacle. The injection opening reaches the first face, the flow channel is in fluid communication between the injection opening and the reaction receptacle, and the flow channel is in fluid communication with the injection opening through a flow channel opening. The blocking element is vertically movably disposed in the injection opening, and the blocking element selectively closes the flow channel opening. Therefore, the back flow of a sample can be prevented, so as to ensure chemical reaction of the sample and a reagent and thus improve accuracy of the test results.

A device is disclosed for conducting a non-invasive analysis of a bodily fluid to determine the presence and level of a certain constituent carried by the bodily fluid. An indicator formulation of the device changes color in response to exposure to the constituent to provide a visible indication of the presence and level of the constituent carried by the bodily fluid. A carrier substrate of the device is constructed of a material having voids providing a high void volume within the substrate. The device is made by applying a chromagen to the carrier substrate to create a chromagen-laden carrier member. Then, a selected reagent having a particular constituent-specific formulation is applied to the chromagen-laden member. The selected reagent then combines with the chromagen thereby establishing the indicator formulation within the carrier substrate in place for reception of a sample of the bodily fluid.

A device is disclosed for conducting a non-invasive analysis of a bodily fluid to determine the presence and level of a certain constituent carried by the bodily fluid. An indicator formulation of the device changes color in response to exposure to the constituent to provide a visible indication of the presence and level of the constituent carried by the bodily fluid. A carrier substrate of the device is constructed of a material having voids providing a high void volume within the substrate. The device is made by applying a chromagen to the carrier substrate to create a chromagen-laden carrier member. Then, a selected reagent having a particular constituent-specific formulation is applied to the chromagen-laden member. The selected reagent then combines with the chromagen thereby establishing the indicator formulation within the carrier substrate in place for reception of a sample of the bodily fluid.

A multi-fluid test strip may include a first zone to test a first fluid, a second zone to test a second fluid, and a third zone intermediate the first zone and the second zone. The third zone may prevent cross-contamination of the first fluid with the second zone and prevent cross-contamination of the second fluid with the first zone. The multi-fluid test strip may also include a first grip zone and a second grip zone. The first zone may be intermediate the first grip zone and the third zone and the second zone may be intermediate the second grip zone and the third zone. The first grip and second grip zones may be dimensioned to permit gripping of the first and second grip zones without touching the first or second zones.