A CMOS-based chip having one or more sensing modalities that are able independently to detect multiple metabolites present in a biological sample. The multiple sensing modalities may be provided at different locations with respect to the chip, whereby the chip can simultaneously detect a plurality of metabolites by measuring behaviour of a test material in the different locations. The chip may utilise paper as a transport mechanism for the sample. The paper either conveys the sample to the different locations or itself provides discrete testing zones in which different metabolites can be independently detected. With this technique, multiple metabolites may be measured in real time using a small scale point-of-care device.

A CMOS-based chip having one or more sensing modalities that are able independently to detect multiple metabolites present in a biological sample. The multiple sensing modalities may be provided at different locations with respect to the chip, whereby the chip can simultaneously detect a plurality of metabolites by measuring behaviour of a test material in the different locations. The chip may utilise paper as a transport mechanism for the sample. The paper either conveys the sample to the different locations or itself provides discrete testing zones in which different metabolites can be independently detected. With this technique, multiple metabolites may be measured in real time using a small scale point-of-care device.

Biological research requires isolation and analysis of material, for example, RNA, DNA and protein, from tissue samples. The methods and compositions described herein allow for high resolution imaging of large and intact tissue samples, and subsequent isolation of material in a precise and location dependent-manner. The methods and compositions described herein may be used, for example, for biomarker discovery, identification of cell populations, pathology analysis, and generation of expression data in specific regions of interest.

Described herein are novel rapid and reliable methods of detection of extracellular vesicles and quantifying extracellular vesicle concentrations and absolute number from various sources, including raw cell harvest. The methods described herein comprise detection of intrinsic fluorescence of extracellular vesicles in biological samples. Extracellular vesicles analyzed by the methods of this application have a stereotypical elution profile distinct from known contaminants. The methods described herein are a significant improvement over the state of the art and fulfills an unmet need in the field of extracellular vesicle manufacturing and quality control.

Described herein are novel rapid and reliable methods of detection of extracellular vesicles and quantifying extracellular vesicle concentrations and absolute number from various sources, including raw cell harvest. The methods described herein comprise detection of intrinsic fluorescence of extracellular vesicles in biological samples. Extracellular vesicles analyzed by the methods of this application have a stereotypical elution profile distinct from known contaminants. The methods described herein are a significant improvement over the state of the art and fulfills an unmet need in the field of extracellular vesicle manufacturing and quality control.

The present invention provides a biomolecule image sensor in which detection molecules are deposed on a light receiving surface of an image sensing element, and method thereof for detecting biomolecule.

The present invention provides a biomolecule image sensor in which detection molecules are deposed on a light receiving surface of an image sensing element, and method thereof for detecting biomolecule.

A CuInS.sub.2@ZnS nanomaterial synthesized with thiosalicylic acid and sodium citrate as dual-stabilizers is taken as a chemiluminescent luminophore, and Tris buffer containing both N.sub.2H.sub.4.H.sub.2O and H.sub.2O.sub.2 is taken as the triggering solution; introducing the H.sub.2O.sub.2 into the triggering solution can bring out greatly enhanced CL emission and obviously shortened CL process, enable the CuInS.sub.2@ZnS nanomaterial with strong flash-type and near-infrared CL; the luminophore of CuInS.sub.2@ZnS nanomaterial is synthesized by a one-pot method; compared with acridinium ester (a classical flash-type chemiluminescent substance), the CuInS.sub.2@ZnS nanomaterial is simple in synthesis method, mild in conditions and short in the required time, the synthesized CuInS.sub.2@ZnS nanomaterial is not easy to decompose under light, and the CL waveband is in the near-infrared region.

A CuInS.sub.2@ZnS nanomaterial synthesized with thiosalicylic acid and sodium citrate as dual-stabilizers is taken as a chemiluminescent luminophore, and Tris buffer containing both N.sub.2H.sub.4.H.sub.2O and H.sub.2O.sub.2 is taken as the triggering solution; introducing the H.sub.2O.sub.2 into the triggering solution can bring out greatly enhanced CL emission and obviously shortened CL process, enable the CuInS.sub.2@ZnS nanomaterial with strong flash-type and near-infrared CL; the luminophore of CuInS.sub.2@ZnS nanomaterial is synthesized by a one-pot method; compared with acridinium ester (a classical flash-type chemiluminescent substance), the CuInS.sub.2@ZnS nanomaterial is simple in synthesis method, mild in conditions and short in the required time, the synthesized CuInS.sub.2@ZnS nanomaterial is not easy to decompose under light, and the CL waveband is in the near-infrared region.

This invention is in the field of medical devices. Specifically, the present invention provides fluidic systems having a plurality of reaction sites surrounded by optical barriers to reduce the amount of optical cross-talk between signals detected from various reaction sites. The invention also provides a method of manufacturing fluidic systems and methods of using the systems.