A reagent strip and a reagent analyzer for reading the reagent strip is described. The reagent strip includes a substrate, at least one reagent pad positioned on the substrate, and a photo luminescent phosphor spot positioned at a fixed location on the substrate. The photo luminescent phosphor spot is formulated to exhibit a predetermined addressable attribute.

A method is provided for degradation-compensated evaluation of detection signals of a sensor arrangement operating on the principle of luminescence quenching, which arrangement has a luminophore that degrades over time, an excitation radiation source, and at least one optical sensor. The luminophore radiates, in accordance with a response characteristic of the sensor arrangement, in reaction to irradiation with a predefined modulated excitation radiation and as a function of the extent of an interaction of the luminophore with a quencher substance that quenches the luminescence of the luminophore. A response radiation is detected by the at least one optical sensor. The sensor arrangement outputs a detected intensity value representing an intensity of the response radiation and a detected phase value representing a phase difference of the response radiation with respect to the modulation of the excitation radiation. A predetermined calibration value correlation is identified in consideration of the reference response characteristic.

A method is provided for degradation-compensated evaluation of detection signals of a sensor arrangement operating on the principle of luminescence quenching, which arrangement has a luminophore that degrades over time, an excitation radiation source, and at least one optical sensor. The luminophore radiates, in accordance with a response characteristic of the sensor arrangement, in reaction to irradiation with a predefined modulated excitation radiation and as a function of the extent of an interaction of the luminophore with a quencher substance that quenches the luminescence of the luminophore. A response radiation is detected by the at least one optical sensor. The sensor arrangement outputs a detected intensity value representing an intensity of the response radiation and a detected phase value representing a phase difference of the response radiation with respect to the modulation of the excitation radiation. A predetermined calibration value correlation is identified in consideration of the reference response characteristic.

A method of detecting an amine-based additive in a wellbore servicing fluid (WSF) comprising contacting an aliquot of WSF with an amine detector compound and an aqueous salt solution to form a detection solution; wherein the aqueous salt solution comprises an inorganic salt and organic carboxylate salt; wherein the WSF comprises the amine-based additive; and wherein the detection solution is characterized by at least one absorption peak wavelength in the range of 380-760 nm; detecting an absorption intensity for detection solution at a wavelength within about ±20% of the at least one absorption peak wavelength; comparing the absorption intensity of detection solution at the wavelength within about ±20% of the at least one absorption peak wavelength with a target absorption intensity of amine-based additive to determine the amount of amine-based additive in WSF; and comparing the amount of amine-based additive in WSF with a target amount of amine-based additive.

A method of detecting an amine-based additive in a wellbore servicing fluid (WSF) comprising contacting an aliquot of WSF with an amine detector compound and an aqueous salt solution to form a detection solution; wherein the aqueous salt solution comprises an inorganic salt and organic carboxylate salt; wherein the WSF comprises the amine-based additive; and wherein the detection solution is characterized by at least one absorption peak wavelength in the range of 380-760 nm; detecting an absorption intensity for detection solution at a wavelength within about ±20% of the at least one absorption peak wavelength; comparing the absorption intensity of detection solution at the wavelength within about ±20% of the at least one absorption peak wavelength with a target absorption intensity of amine-based additive to determine the amount of amine-based additive in WSF; and comparing the amount of amine-based additive in WSF with a target amount of amine-based additive.

An example biosensor includes a substrate, a graphene layer disposed on the substrate, and a binding site bonded to the graphene. The binding site includes an antibody configured to bind a SARS-CoV-2 spike glycoprotein.

A method of detecting an amine-based additive in wellbore servicing fluid (WSF) comprising contacting an aliquot of WSF with an amine detector reagent and aqueous medium to form a detection solution; wherein the amine detector reagent comprises an amine detector compound, and a polar organic solvent (POS) with flash point >105° C.; wherein the WSF comprises the amine-based additive; and wherein the detection solution is characterized by at least one absorption peak wavelength in 380-760 nm; detecting an absorption intensity for detection solution at a wavelength within ±20% of the at least one absorption peak wavelength; comparing the absorption intensity of detection solution at the wavelength within ±20% of the at least one absorption peak wavelength with a target absorption intensity of amine-based additive to determine the amount of amine-based additive in WSF; and comparing the amount of amine-based additive in WSF with a target amount of amine-based additive.

A method of detecting an amine-based additive in wellbore servicing fluid (WSF) comprising contacting an aliquot of WSF with an amine detector reagent and aqueous medium to form a detection solution; wherein the amine detector reagent comprises an amine detector compound, and a polar organic solvent (POS) with flash point >105° C.; wherein the WSF comprises the amine-based additive; and wherein the detection solution is characterized by at least one absorption peak wavelength in 380-760 nm; detecting an absorption intensity for detection solution at a wavelength within ±20% of the at least one absorption peak wavelength; comparing the absorption intensity of detection solution at the wavelength within ±20% of the at least one absorption peak wavelength with a target absorption intensity of amine-based additive to determine the amount of amine-based additive in WSF; and comparing the amount of amine-based additive in WSF with a target amount of amine-based additive.

A multifunctional biosensor is described that is configured to simultaneously detect two or more different types of chemical, biological and/or radiological/nuclear (CBRN) threats on one platform using FRET-based and/or NSET-based technology.

A multifunctional biosensor is described that is configured to simultaneously detect two or more different types of chemical, biological and/or radiological/nuclear (CBRN) threats on one platform using FRET-based and/or NSET-based technology.