An injection valve switches between a first state in which a sample loop is connected to a separation flow path through which a mobile phase from a liquid sender flows and a second state in which the sample loop is disconnected from the separation flow path. A pump speed determiner, in a case where a sample intake operation by a syringe pump is started immediately after the injection valve is switched from the second state to the first state, and a filling operation of filling the sample loop with the sample by the syringe pump is started after the intake operation is completed, determines an intake operation speed of the syringe pump that is required in order for the filling operation to complete immediately before the injection valve is switched from the second state to the first state next time using a set injection interval time. The syringe pump operates at the intake operation speed determined by the pump speed determiner while performing the intake operation. The sample is injected by the injector at intervals of the set injection interval time.

An injection valve switches between a first state in which a sample loop is connected to a separation flow path through which a mobile phase from a liquid sender flows and a second state in which the sample loop is disconnected from the separation flow path. A pump speed determiner, in a case where a sample intake operation by a syringe pump is started immediately after the injection valve is switched from the second state to the first state, and a filling operation of filling the sample loop with the sample by the syringe pump is started after the intake operation is completed, determines an intake operation speed of the syringe pump that is required in order for the filling operation to complete immediately before the injection valve is switched from the second state to the first state next time using a set injection interval time. The syringe pump operates at the intake operation speed determined by the pump speed determiner while performing the intake operation. The sample is injected by the injector at intervals of the set injection interval time.

An analyzing device includes a splitting part for causing fluid containing a sample component to flow separately in a first flow passage and a second flow passage; an analyzing column provided on the first flow passage for separating the sample component from the fluid; a first back pressure regulating valve corresponding to a first pressure controlling unit for controlling a pressure in the first flow passage; and a second back pressure regulating valve corresponding to a second pressure controlling unit for controlling a pressure in the second flow passage, wherein flow rate of the fluid in the first flow passage and flow rate of the fluid in the second flow passage are controlled based on a ratio of the pressure in the first flow passage to the pressure in the second flow passage.

A gas-liquid separator according to an embodiment of the present invention separates a mobile phase containing a gas and a liquid into a gas and a liquid. The gas-liquid separator according to the embodiment of the present invention includes an introduction flow channel to which a mobile phase is introduced, and a plurality of discharge flow channels connected to the introduction flow channel. A gas and a liquid are discharged from a discharge port of the discharge flow channel.

A gas-liquid separator according to an embodiment of the present invention separates a mobile phase containing a gas and a liquid into a gas and a liquid. The gas-liquid separator according to the embodiment of the present invention includes an introduction flow channel to which a mobile phase is introduced, and a plurality of discharge flow channels connected to the introduction flow channel. A gas and a liquid are discharged from a discharge port of the discharge flow channel.

Provided is a preparative separation liquid chromatograph system and preparative separation condition searching method which allows for an easy setting of the preparative separation condition. A sample temporally separated into components by a separation column is introduced into a detector and a fraction collector, with each component fractionated and collected by the fraction collector based on the result of a detection by the detector. A controlling and processing unit holds the following data for each sample or compound in the form of a database: chromatogram data obtained when a liquid chromatograph analysis in a preparative separation condition searching mode is performed for various standard samples under a search condition; and chromatogram data obtained when a liquid chromatograph analysis in a preparative separation mode is performed under one or more sets of preparative separation conditions for the various standard samples, along with the preparative separation condition used in this analysis.

Provided is a preparative separation liquid chromatograph system and preparative separation condition searching method which allows for an easy setting of the preparative separation condition. A sample temporally separated into components by a separation column is introduced into a detector and a fraction collector, with each component fractionated and collected by the fraction collector based on the result of a detection by the detector. A controlling and processing unit holds the following data for each sample or compound in the form of a database: chromatogram data obtained when a liquid chromatograph analysis in a preparative separation condition searching mode is performed for various standard samples under a search condition; and chromatogram data obtained when a liquid chromatograph analysis in a preparative separation mode is performed under one or more sets of preparative separation conditions for the various standard samples, along with the preparative separation condition used in this analysis.

A supercritical fluid chromatography system comprises a first pump for pumping a first flow stream comprising a compressible fluid and a second pump for pumping a second flow stream comprising a modifier fluid. The second pump is in parallel with the first pump. A column is located in a combined flow stream. The column is located downstream of the first and second pumps. The combined flow stream comprises the first flow stream, the second flow stream, and a sample. A detector is located downstream of the column. A gas-liquid separator is located downstream of the detector. The gas-liquid separator is configured to vent a majority of the compressible fluid while maintaining a majority of the sample, thus preventing aerosolization of the flow stream and minimizing sample loss as well as cross contamination. An open bed collector is located after the gas-liquid separator.

A supercritical fluid chromatography system comprises a first pump for pumping a first flow stream comprising a compressible fluid and a second pump for pumping a second flow stream comprising a modifier fluid. The second pump is in parallel with the first pump. A column is located in a combined flow stream. The column is located downstream of the first and second pumps. The combined flow stream comprises the first flow stream, the second flow stream, and a sample. A detector is located downstream of the column. A gas-liquid separator is located downstream of the detector. The gas-liquid separator is configured to vent a majority of the compressible fluid while maintaining a majority of the sample, thus preventing aerosolization of the flow stream and minimizing sample loss as well as cross contamination. An open bed collector is located after the gas-liquid separator.

The disclosure relates to a gradient twin column recycling chromatography method that is used to separate a mixture containing closely eluting compounds. In one embodiment, a sample includes a primary organic compound and one or more impurities that closely elute with the primary organic compound. A gradient mobile phase is initially used to remove unwanted early eluting and late eluting impurities from the sample. After the gradient removal of some of the impurities is complete, the remaining mixture of the primary organic compound and the closely eluting impurities are separated using recycle chromatography methodology with an isocratic mobile phase.