A field testing device is provided for quantitating components of marijuana such as THC, THC-A, CBD, CBDA, and/or CBN. Quantitation may be made, e.g. from biological fluids such as saliva, or from plant extracts. A device according to the invention may include an HPTLC plate for spatially separating interferents and analytes, and may also include fluorometric components for quantitating analytes. The device may include a microprocessor adapted to relate fluorescent intensity to analyte concentration through one or more calibration curves. Devices may optionally include microfluidics for carrying out HPTLC on biological samples including sample reservoirs, reagent reservoirs, micro-pumps, mixers, and the like.

A method for the automatic chromatography of thin-layer plates for thin-layer chromatography with a development chamber 1 in which a thin-layer plate D is completely enclosed, sealed-off and isolated from the external environment. In the development chamber, a front space containing an inner atmosphere is located on the front face of the separation layer of the thin-layer plate. The depth of the front space is about 2 mm and a maximum 3 mm. An inlet is provided at one end of the front space and an outlet is provided at the other end of the front space. During the chromatographic development, a stream of gas of particular composition determined by the user is created throughout the entire front space, the entire inner atmosphere being set in motion, without stagnant or stationary gas phase.

A method for the automatic chromatography of thin-layer plates for thin-layer chromatography with a development chamber 1 in which a thin-layer plate D is completely enclosed, sealed-off and isolated from the external environment. In the development chamber, a front space containing an inner atmosphere is located on the front face of the separation layer of the thin-layer plate. The depth of the front space is about 2 mm and a maximum 3 mm. An inlet is provided at one end of the front space and an outlet is provided at the other end of the front space. During the chromatographic development, a stream of gas of particular composition determined by the user is created throughout the entire front space, the entire inner atmosphere being set in motion, without stagnant or stationary gas phase.

A method for rapidly detecting pesticides based on thin-layer chromatography (TLC) and enzyme inhibition principles. The method includes the following steps: cutting a TLC plate into a rectangle, and using one end of the rectangle to contact a sample extract to form a pesticide residue separation area; covering the other end of the rectangle with a small piece cut from filter paper or glass fiber and fixing on a piece of enzyme inhibition reaction test paper to form a pesticide enrichment area; pasting a side of the enzyme inhibition reaction test paper away from the pesticide residue separation area with a piece of filter paper immobilized with a chromogenic agent to form a substrate color development area; and performing color reaction.

A method for rapidly detecting pesticides based on thin-layer chromatography (TLC) and enzyme inhibition principles. The method includes the following steps: cutting a TLC plate into a rectangle, and using one end of the rectangle to contact a sample extract to form a pesticide residue separation area; covering the other end of the rectangle with a small piece cut from filter paper or glass fiber and fixing on a piece of enzyme inhibition reaction test paper to form a pesticide enrichment area; pasting a side of the enzyme inhibition reaction test paper away from the pesticide residue separation area with a piece of filter paper immobilized with a chromogenic agent to form a substrate color development area; and performing color reaction.

A field testing device is provided for quantitating psychoactive components of marijuana such as THC in biological fluids such as saliva. Such a device may include an HPTLC plate for separating interferents from THC and may also include fluorometric components for quantitating THC. The device may include a microprocessor adapted to relate fluorescent intensity to analyte concentration through one or more calibration curves. Devices may optionally include microfluidics for carrying out HPTLC on biological samples including sample reservoirs, reagent reservoirs, micro-pumps, mixers, and the like.

A field testing device is provided for quantitating psychoactive components of marijuana such as THC in biological fluids such as saliva. Such a device may include an HPTLC plate for separating interferents from THC and may also include fluorometric components for quantitating THC. The device may include a microprocessor adapted to relate fluorescent intensity to analyte concentration through one or more calibration curves. Devices may optionally include microfluidics for carrying out HPTLC on biological samples including sample reservoirs, reagent reservoirs, micro-pumps, mixers, and the like.

A derivatization apparatus and method for coating a sample carrier with a reagent solution. The derivatization apparatus consists of a closed system in which a small quantity of reagent solution is sprayed into a closed container via a spray nozzle. A sample carrier is located in the closed container and is coated as homogeneously as possible with the reagent solution.

A derivatization apparatus and method for coating a sample carrier with a reagent solution. The derivatization apparatus consists of a closed system in which a small quantity of reagent solution is sprayed into a closed container via a spray nozzle. A sample carrier is located in the closed container and is coated as homogeneously as possible with the reagent solution.

The thin layer chromatography plate includes a substrate and a separation layer. The separation layer is disposed on the substrate and is configured to separate multiple components included in a sample from each other. The separation layer includes a first layer and a second layer. The first layer has a porous structure and extends in a first direction. The second layer has a porous structure and extends in the first direction. The first layer and the second layer are arrayed in a second direction orthogonal to the first direction. A zeta potential of the first layer is different from a zeta potential of the second layer.