Disclosed herein are systems and methods for imaging cells. Quantitative phase imaging uses variations in the index of refraction of a sample as a source of endogenous contrast, providing label-free information of sub-cellular structures and allowing for the reconstruction of valuable biophysical parameters, such as cell dry-mass at femtogram scales, mass transport, and sample thickness and fluctuations at nanometer scales. As a result, QPI has become a valuable tool in biology and medicine. However, QPI has suffered from the need for trans-illumination through relatively thin objects in order to gain access to the forward-scattered field, which carries crucial low spatial frequency information of a sample and avoid contributions from multiple scattered light or out-of-focus planes. The disclosed methods and systems can provide for reconstruction of QPI and corresponding analysis for imaging samples of cells in thick samples using an epi-illumination configuration.

Cable handling devices, methods, and systems for securing and deploying or retracting one or more cables or hoses into or out of a pipe or cavity allowing inspection of the pipe or cavity. In an exemplary embodiment, a hand-held device with a housing for at least partially enclosing one or more cables or hoses, at least one coupling mechanism integrally attached to the housing, and a coupling control for coupling and decoupling the one or more cables or hoses is provided. The cable handling device and the one or more cables or hoses can be coupled and moved together, or decoupled enabling the cable handling device and the one or more cables or hoses to move independently of each other. One or more of the cables may be connected to a camera for facilitating inspection of a pipe or cavity.

An interferometric method and apparatus for the non-invasive assessment of oocyte maturity and competency. The method includes placing an oocyte in a sample holder to provide a biological target; generating a near infrared light; using a beam splitter to split the near infrared light into a signal light portion and a reference light portion; projecting the signal light portion onto the biological target; collecting reflected and back scattered light from the signal light portion projected onto the biological target with a detector; collecting at least a portion of the reference light portion with the detector; generating interferometric image data based upon the collected signal and reference light; and assessing the maturity of the oocyte based upon the interferometric data while maintaining the viability of the oocyte. When the oocyte is part of a cumulus-oocyte complex, the assessment is accomplished without removing cumulus cells from the cumulus-oocyte complex while maintaining viability.

Cell counters and methods of their use are disclosed herein. The cell counters comprise a sample mounting system that includes a base comprising a mounted lower sample surface and a cover comprising a mounted upper sample surface; a bright-field light source incorporated in the cover; an objective lens mounted below the sample mounting system; optionally, a fluorescence excitation source in optical communication with the sample mounting system; and an imaging system in optical communication with the bright-field light source and the objective lens. The mounted sample surfaces are configured for repeated use, such that disposable sample cartridges are not needed.

An optical spectral sensing device for determining at least one property of a fluid. The device has an elongated porous body, a first end and a second end, a solid-state optical emitter at the first end of the body oriented to emit radiation toward the second end of the body, and a solid-state optical detector at the second end of the body oriented to detect radiation emitted by the optical emitter and to output a signal responsive to absorption of radiation. The device is configured to determine depth of a fluid based on the signal output by the optical detector.

An optical blood monitoring system and corresponding method avoid the need to obtain a precise intensity value of the light impinging upon the measured blood layer during the analysis. The system is operated to determine at least two optical measurements through blood layers of different thickness but otherwise substantially identical systems. Due to the equivalence of the systems, the two measurements can be compared so that the bulk extinction coefficient of the blood can be calculated based only on the known blood layer thicknesses and the two measurements. Reliable measurements of various blood parameters can thereby be determined without certain calibration steps.

Described herein are systems and methods for extending the dynamic range of assay methods and systems used for determining the concentration of analyte molecules or particles in a fluid sample. In some embodiments, a method comprises spatially segregating a plurality of analyte molecules in a fluid sample into a plurality of locations. At least a portion of the locations may be addressed to determine the percentage of said locations containing at least one analyte molecule. Based at least in part on the percentage, a measure of the concentration of analyte molecules in the fluid sample may be determined using an analog, intensity-based detection/analysis method/system and/or a digital detection/analysis method/system. In some cases, the assay may comprise the use of a plurality of capture objects.

A device for automatically checking quality of a spool of thread for fabrics is provided. The device has at least one vision system provided with a camera, a frame of the camera defining an analysis area, the camera being connectable to a system for moving a spool of thread to be analyzed and to a computer having an analysis software stored thereon, and at least one lighting system having at least one pair of lights arranged facing each other and transversely in relation to the analysis area, each light of the at least one pair of lights having at least one light source for providing a sidelight to the analysis area.

The present invention relates generally to the field of chemical and biological sensors and in particular to micro electro-mechanical systems (MEMS) sensors for measuring fluid viscosity and detection of minute amounts of chemicals and biological agents in fluids. It is an object of the present invention to provide a sensor that will work in disposable cartridges with remote sensing that can measure dynamic changes of the functionalized cantilevers in liquid and gas environment.

A garment-mountable physiological parameter detector, comprising: an illuminator configured to illuminate in the direction of the garment; a photoresistor configured to receive light reflections from the garment; and an electronic circuit configured to detect a bodily secretion by monitoring the light reflections received by said photoresistor and identifying a temporal pattern being characteristic of a bodily secretion event.