A method of obtaining a quantum efficiency distribution in a predetermined sample surface, including: irradiating a reference material with excitation light belonging to a first wavelength range; obtaining the reference material's image, which includes a first channel for the first wavelength range and a second channel for a second wavelength range, the first and the second channel's irradiation luminance value in each pixel; irradiating the predetermined sample surface with the excitation light; obtaining the first and the second channel's measurement luminance value in each pixel of the image of the predetermined surface; calculating an absorption luminance value from a difference between the first channel's irradiation luminance value and measurement luminance value; calculating a fluorescence luminance value from difference between the second channel's irradiation luminance value and measurement luminance value; calculating quantum efficiency of each pixel based on the values; and obtaining quantum efficiency distribution.

A seamless fumed silica monolithic integrating cavity device tailored to analyzing a flowed sample. The device is configured to facilitate optical measurements taken from a sample flowed through a cavity of the device. The cavity is defined by a fumed silica monolith with the added feature of a fused quartz lining on the surface of the monolith. This provides an intermediate surface that allows for cleaning and reuse of the highly effective diffuse light scattering fumed silica monolith. The lining may be placed under pressure or vacuum to structurally enhance mechanical integrity of the underlying monolith. Thus, continued or reliably repeated use of the device may be appreciated as well as use in more industrial environments that are prone to vibration. Additionally, while well suited for flow-based sample analysis, a valve of the cavity may be utilized for holding a sample in a temporarily static state for measurement.

Provided herein is an apparatus for assessing a color characteristic of a gemstone. The apparatus comprises an optically opaque platform for supporting a sample gemstone to be assessed, a daylight-approximating light source to provide uniform illumination to the gemstone, an image capturing component, and a telecentric lens positioned to provide an image of the illuminated gemstone to the image capturing component. Also provided are methods of color analysis based on images collected using such an apparatus.

In a spectrometry device, a control unit controls a light source so that input of excitation light to an internal space is maintained in a first period, and that the input of the excitation light to the internal space is stopped in a second period, and the analysis unit calculates the photoluminescence quantum yield of a long afterglow emission material on the basis of the number of absorbed photons of the long afterglow emission material obtained on the basis of excitation light spectral data in the first period and the number of light emission photons of the long afterglow emission material obtained on the basis of light emission spectral data in any of the first period, the second period, and a total period of the first period and the second period.

An optical characteristic measurement device has a measurement opening, includes a first optical measurement unit and a second optical measurement unit that measure different optical characteristics with different geometries with respect to a measurement target facing the measurement opening, and further includes a processing unit that corrects a measurement value obtained in the second optical measurement unit based on a measurement value obtained in the first optical measurement unit. The first optical measurement unit includes an illumination optical system that illuminates the measurement target facing the measurement opening, a first light receiving optical system that collects light reflected by the measurement target, and a first light receiving unit that receives light collected by the first light receiving optical system and outputs the light as a measurement signal, and has a diffuse reflection surface that diffuses and reflects incident light to the illumination optical system or the first light receiving optical system. The second optical measurement unit includes a light projecting optical system that projects light from a direction inclined by a predetermined angle with respect to a normal line of a measurement surface of the measurement target facing the measurement opening, a second light receiving optical system that collects light reflected by the measurement target in a regular reflection direction, and a second light receiving unit that receives light collected by the second light receiving optical system and outputs the light as a measurement signal.

The optical reader for analyzing biological samples comprises a reading plane (3) for receiving a microplate (1), an illuminating arrangement (4) configured to illuminate samples in the wells (2) of the microplate (1), an imaging device (6) arranged to receive light from the microplate (1), a beam splitter (7), which is arranged to direct light from the illuminating arrangement (4) towards the reading plane (3) and to direct light received from the microplate (1) to the imaging device (6), and a lens system (8) arranged between the beam splitter (7) and the reading plane (3) to focus the light received from the illuminating arrangement (4) to a sample and to focus an image of the sample to the imaging device (6). The optical reader is configured to transmit from the illuminating arrangement (4) to the lens system (8) only light having a specific polarization, and the optical reader comprises a polarizer (10, 19) that is arranged between the lens system (8) and the imaging device (6) and configured to block polarized light reflected from the surfaces of the lens system (8).

The method of analyzing samples (3) in the wells (2) of a microplate (1) comprises the steps of producing electromagnetic radiation having a first predetermined wavelength or wavelength range (101), illuminating a sample (3) by said radiation by transmitting the radiation to the sample (3) from above the microplate (1) via the upper end of the well (102), transmitting light emitted by the sample (3) and having a second predetermined wavelength or wavelength range via the upper end of the well (2) to detection means (13) (103), determining intensity of light emitted by one or more predetermined measurement areas (23) of the sample (3) (104), based on the determined intensities, determining a result value representing the total amount of light emitted by the sample (3), and counting the number of spots emitting light having the second predetermined wavelength or wave-length range (106).

Provided is a portable biosensor that includes a sample filter cartridge, a filter collector, an optical sphere, an electromagnetic radiation emitter, a photo-detector, a processor, a signal display, a vacuum pump, and a power supply. The sample filter cartridge selectively removes small molecules to minimize spectral interference in the detection signal. The sample is concentrated onto the filter collector and subjected to illumination by the electromagnetic radiation emitter, producing Raman-scattering. The optical sphere collects and distributes the Raman-scattering shifts, which then pass through a spectral filter to produce spectral filtered scattering, which is then reflected by the concave holographic flat-field grating onto the photo-detector. The data is displayed graphically to provide the Raman-scattering shift data. The data is compared with a database for sample identification. The device is contained within a housing that is small enough to be easily transported for field use.

A device and a method for simultaneously inspecting defects of a surface and a subsurface of an optical element are provided. Combined with laser-induced ultrasound and laser scattering inspection technologies, through generating acoustic sound waves on the surface and the subsurface of the optical element to be tested by lasers, a static light scattering effect of subsurface defects under modulation of the acoustic sound wave is observed and analyzed; through analyzing amplitude and phase changes of scattered light intensity and reflected light intensity, inspection for the defects of the surface and the subsurface of the optical element is realized. The present invention can be applied in quality inspection of precise optical elements, especially in finished product inspection of ultra-precise optical elements having strict requirements on the subsurface defects.

The invention discloses a portable visible/near-infrared spectrum detection device, comprising a housing, a bottom plate, a screen support frame, a battery part on which a microprocessor is arranged, a detection part and a switch part, wherein the battery part, the detection part and the switch part are arranged in the housing; and the detection part comprises a spectrometer, a light source, a collimating mirror and the microprocessor. The device has the characteristics of rapidness, no damage and portability, and can realize rapid detection and early warning of food quality.