The present invention relates to a pillar structure for a biochip and includes a substrate which has a plate-shaped structure, and pillar members, each of which has one side detachably coupled to the substrate and the other side on which a sample is disposed.

This detection device has a holder, light irradiation unit, angle adjustment unit, light receiving sensor, light receiving optical system, optical filter, and a control unit. The light receiving optical system guides light from a detection chip to the light receiving sensor. The optical filter is disposed in the light receiving optical system, blocks a part of plasmon scattered light, and passes, out of the light emitted from the detection chip, a part of the plasmon scattered light, and fluorescence emitted from a fluorescent material. The light receiving sensor detects the fluorescent light, and the part of the plasmon scattered light, which have been emitted from the detection chip and passed the optical filter. On the basis of the detection results of the plasmon scattered light, the control unit controls the angle adjustment unit, and adjusts the incident angle of the excitation light to a predetermined incident angle.

The flow cell of the present application simultaneously monitors and measures light absorbance and fluorescence of particles in a flowing liquid. The flow cell comprises a housing having a light input face, an absorbance output face and first and second emission output faces; a fluid flow section within the housing that comprises a bottom funnel through which fluid enters the flow cell, a core chamber into which fluid flows from the bottom funnel, and a top funnel into which fluid flows from the core chamber, wherein the bottom and top funnels each comprise a first end which extends at an angle to a second end that is wider in diameter than the first end, and said second end of each is adjacent to and aligned with the core chamber; and a center section within the housing center having a recess formed therein which houses the core chamber of the fluid flow section, wherein said center section comprises a first pair of opposing channels formed in the light input face and the absorbance output face, respectively, and a second pair of opposing channels formed in the first emission output face and the second emission output face and which are perpendicular to the first pair of opposing channels, and wherein the first pair of opposing channels and second pair of opposing channels are in communication with the core chamber. An apparatus comprising the flow cell is also provided.

In one example in accordance with the present disclosure, a fluorescence detection system is described. The fluorescence detection system includes a microfluidic chamber to receive a sample containing a compound to be detected. The system also includes a light guide, a portion of which is adjacent the microfluidic chamber. The light guide refracts excitation light into the microfluidic chamber to excite fluorophores in the microfluidic chamber. The light guide has total internal reflection in those portions that are not adjacent the microfluidic chamber. The fluorescence detection system also includes a heating element to trigger a reaction in the microfluidic chamber, an illumination source to provide the excitation, and a detection system to detect fluorescence generated by the excitation of the fluorophores in the microfluidic chamber.

A seamless fumed silica monolithic integrating cavity device tailored to analyzing a flowed sample. The device is configured to facilitate optical measurements taken from a sample flowed through a cavity of the device. The cavity is defined by a fumed silica monolith with the added feature of a fused quartz lining on the surface of the monolith. This provides an intermediate surface that allows for cleaning and reuse of the highly effective diffuse light scattering fumed silica monolith. The lining may be placed under pressure or vacuum to structurally enhance mechanical integrity of the underlying monolith. Thus, continued or reliably repeated use of the device may be appreciated as well as use in more industrial environments that are prone to vibration. Additionally, while well suited for flow-based sample analysis, a valve of the cavity may be utilized for holding a sample in a temporarily static state for measurement.

A light guide device for conducting a light beam between a light source and a measuring unit for measuring a gas or substance concentration includes a light conductor and a holding apparatus. The conductor includes at least one coupling section, which faces, or can be arranged to be turned toward, the light source, for coupling the light beam, and a decoupling section, which faces, or can be arranged to be turned toward, the measuring unit, for decoupling the light beam. The conductor is configured to conduct the light beam between the coupling section and the decoupling section via total reflection on a boundary surface to a fluid or material that surrounds the conductor and has a smaller refractive index than the conductor. The holding apparatus is configured to hold the conductor in the fluid such that at least one primary portion of a surface of the conductor contacts the fluid.

The present invention provides a multi-channel fluorescence detecting system for detecting a plurality of fluorescence labeled analytes. The multi-channel fluorescence detecting system comprises a light source, a light filter device, a dual branch light guide tube, and a detector. The light source comprises a plurality of sub light sources for respectively providing an excitation light. The plurality of sub light sources are a plurality of single color Light emitting diodes (LEDs) which can be selectively turned on or off. The light source generates a plurality of lights with full width at half maximum (FWHM) wavelengths formed in a non-overlap manner. With the disposition of the plurality of sub light sources, the accuracy for detecting the specific analytes is raised, the light flux with a specific wavelength band is effectively raised (without raising the light flux of the full wavelength band), the structure is simplified, and the manufacturing cost is decreased.

This detection device has a holder, light irradiation unit, angle adjustment unit, light receiving sensor, light receiving optical system, optical filter, and a control unit. The light receiving optical system guides light from a detection chip to the light receiving sensor. The optical filter is disposed in the light receiving optical system, blocks a part of plasmon scattered light, and passes, out of the light emitted from the detection chip, a part of the plasmon scattered light, and fluorescence emitted from a fluorescent material. The light receiving sensor detects the fluorescent light, and the part of the plasmon scattered light, which have been emitted from the detection chip and passed the optical filter. On the basis of the detection results of the plasmon scattered light, the control unit controls the angle adjustment unit, and adjusts the incident angle of the excitation light to a predetermined incident angle.

Detection system comprising an examination region, a one-piece optical element including a focusing portion to concentrate light received from the examination region and a guiding portion to homogenize light received from the focusing portion, and a detector configured to detect homogenized light received from the guiding portion.

A detection device comprises a chip holder, a light source, a light-guide rod, a wavelength separation filter, and an optical sensor. Given the relationship between the angle of incidence and light intensity of fluorescence on a light reception surface of the optical sensor, the optical transmittance of the wavelength separation filter at the dominant wavelength of the rays of fluorescence incident on the light reception surface at a peak angle of incidence at which the light intensity is the highest is greater than the optical transmittance of the wavelength separation filter at the dominant wavelength of the rays of excitation light incident on the light reception surface at the peak angle of incidence and is higher than the optical transmittance of the wavelength separation filter at the dominant wavelength of the rays of fluorescence incident on the light reception surface at an angle of incidence of 0 DEG.