A Raman spectroscopy system is provided. The spectroscopy system includes an optical switch including a pump inlet, a return outlet, a plurality of pump outlets, and a plurality of return inlets. The spectroscopy system includes a plurality of radiation sources optically coupled to the pump inlet of the optical switch, and a detector optically coupled to the return outlet of the optical switch. The spectroscopy system further includes a plurality of probes, each probe optically connected to at least one of the plurality of pump outlets of the optical switch by at least one excitation fiber and optically coupled to one of the return inlets of the optical switch by at least one emission fiber.

A tunable diode laser absorption spectroscopy (TDLAS) optical head includes a housing configured for attachment to a sight tube attached to a wall of a process chamber. The TDLAS optical head further includes optics within the housing for transmitting, receiving, or transmitting and receiving a laser beam within a process chamber through the sight tube. The TDLAS optical head further includes a photo sensor in the housing positioned to receive light emitted by combustion within the process chamber to which the housing is attached.

A transmission device for examining at least one sample in a microtiter plate, the transmission device including: an illumination device; and a detection device, an intermediate space being formed between the illumination device and the detection device, the intermediate space being configured to receive a microtiter plate. The illumination device including at least one emission source, the illumination device being configured to guide emission light generated by the emission source through the intermediate space. The detection device including at least one detector configured to measure light signals received from the intermediate space; and the detection device includes an angle-dependent filter arranged between the illumination device and the at least one detector in a beam path of the emission light, the angle-dependent filter being configured to substantially only let through light beams having an angle of incidence smaller than a predetermined critical angle.

Systems for measuring a concentration of a target species include a first and second tunable diode laser generating laser light at a respective first and second wavelength each corresponding to respective absorption lines of the target species. A first optical fiber is optically coupled to the first tunable diode laser, and does not support a fundamental mode at the second wavelength. A second optical fiber is coupled to the second tunable diode laser and does not support a fundamental mode at the first wavelength. A fiber bundle includes respective distal ends of the first and second optical fibers, which are stripped of their respective coatings and arranged with their claddings adjacent to each other. A pitch head is configured to project respective optical beams from the fiber bundle through a measurement zone. A catch head located across the measurement zone receives the projected beams and directs them to a sensor.

Disclosed herein are systems and methods for imaging cells. Quantitative phase imaging uses variations in the index of refraction of a sample as a source of endogenous contrast, providing label-free information of sub-cellular structures and allowing for the reconstruction of valuable biophysical parameters, such as cell dry-mass at femtogram scales, mass transport, and sample thickness and fluctuations at nanometer scales. As a result, QPI has become a valuable tool in biology and medicine. However, QPI has suffered from the need for trans-illumination through relatively thin objects in order to gain access to the forward-scattered field, which carries crucial low spatial frequency information of a sample and avoid contributions from multiple scattered light or out-of-focus planes. The disclosed methods and systems can provide for reconstruction of QPI and corresponding analysis for imaging samples of cells in thick samples using an epi-illumination configuration.

The present invention relates to a detection mechanism for polymerase chain reaction and a polymerase chain reaction device, wherein the detection mechanism comprises at least one excitation module group, each of the excitation module groups comprising two excitation modules for providing excitation light with two wavelengths; an excitation optical fiber, connected to the excitation modules, the excitation optical fiber transmitting the excitation light to at least one reaction tube, each of the reaction tubes receiving excitation light with two wavelengths; a receiving optical fiber, for collecting and transmitting a fluorescent signal from the reaction tube; at least one receiving module group, connected to the receiving optical fiber, each of the receiving module groups comprising two receiving modules, to respectively receive the fluorescent signal of two wavelengths from the same said reaction tube, and convert the fluorescent signal into an electrical signal for output; the detection mechanism is configured to detect the reaction tube in a time division manner, and multiplex the receiving module group to obtain an output result.

A spectroscopic system may include: a probe having a probe tip and an optical coupler, the optical coupler including an emitting fiber group and first and second receiving fiber groups, each fiber group having a first end and a second end, wherein the first ends of the fiber groups are formed into a bundle and optically exposed through the probe tip; a light source optically coupled to the second end of the emitting fiber group, the light source emitting light in at least a first waveband and a second waveband, the second waveband being different from the first waveband; a first spectrometer optically coupled to the second end of the first receiving fiber group and configured to process light in the first waveband; and a second spectrometer optically coupled to the second end of the second receiving fiber group and configured to process light in the second waveband.

A method for measuring a concentration of at least one target species includes generating first and second laser beams having respective first and second wavelengths each corresponding to respective absorption lines of the at least one target species. The method includes coupling the first and second laser beams to proximal ends of first and second fundamental modes of first and second optical waveguides, respectively. The method includes transmitting through a measurement zone, for a distal end of the first and second optical waveguides, a probe signal including the first and second laser beam. The method includes determining a first signal strength of the probe signal at the first wavelength and a second signal strength of the probe signal at the second wavelength, and determining, from the first signal strength and the second signal strength, a concentration of the at least one target species.

Provided are an image reconstruction method, a device and a microscopic imaging device. The method includes calculating a gray value at each fiber center in a fiber bundle (04) in a reconstructed image according to a gray value at a center position of each fiber, determined in one or more sample images; performing a spatial interpolation using the gray value at the fiber center to obtain gray values of other pixel points in the fiber bundle (04) in the reconstructed image, so as to form the reconstructed image. This image reconstruction method greatly accelerates the speed of image reconstruction, and is helpful to remove the grating (022) and fiber bundle (04) cellular grid residues in the reconstructed image and improve the imaging quality of the reconstructed image.

An apparatus for analyzing optical properties of a sample includes a housing to receive a light source and a detector; a sample locus defined relative to the housing and positioned such that when a light source and a detector are in predetermined positions, the sample locus is subject to illumination by the light source and the detector is positioned to receive and detect light from the sample; a cover on the housing, the cover being movable in a hinged manner between an open position and a closed position; and a sample-receiving surface for receiving a free-standing sample in liquid or semi-solid form. When the cover is moved to the closed position it encloses the sample locus, with the sample-receiving surface being tilted away from the horizontal during the closing movement and the sample being retained thereon by surface tension or adhesion and brought to the sample locus in an enclosed environment.